Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_68 and Pf6N2E2_69 are separated by 210 nucleotides Pf6N2E2_69 and Pf6N2E2_70 are separated by 337 nucleotides
Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467
_68
Pf6N2E2_69 - Probable type IV pilus assembly FimV-related transmembrane protein, at 109,678 to 112,272
_69
Pf6N2E2_70 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 112,610 to 113,464
_70
Position (kb)
109
110
111
112
113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.195 kb on - strand, within Pf6N2E2_68 at 109.333 kb on + strand, within Pf6N2E2_68 at 109.333 kb on + strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.334 kb on - strand, within Pf6N2E2_68 at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.376 kb on + strand at 109.377 kb on - strand at 109.377 kb on - strand at 109.377 kb on - strand at 109.380 kb on + strand at 109.380 kb on + strand at 109.380 kb on + strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.381 kb on - strand at 109.398 kb on + strand at 109.451 kb on + strand at 109.465 kb on + strand at 109.472 kb on + strand at 109.472 kb on + strand at 109.500 kb on + strand at 109.511 kb on + strand at 109.511 kb on + strand at 109.529 kb on + strand at 109.530 kb on - strand at 109.618 kb on - strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.634 kb on + strand at 109.678 kb on - strand at 109.703 kb on + strand at 109.703 kb on + strand at 109.704 kb on - strand at 109.704 kb on - strand at 109.709 kb on + strand at 109.710 kb on - strand at 110.051 kb on - strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.057 kb on + strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.058 kb on - strand, within Pf6N2E2_69 at 110.164 kb on + strand, within Pf6N2E2_69 at 110.164 kb on + strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.165 kb on - strand, within Pf6N2E2_69 at 110.905 kb on + strand, within Pf6N2E2_69 at 110.905 kb on + strand, within Pf6N2E2_69 at 110.905 kb on + strand, within Pf6N2E2_69 at 111.382 kb on + strand, within Pf6N2E2_69 at 111.383 kb on - strand at 111.553 kb on + strand, within Pf6N2E2_69 at 111.553 kb on + strand, within Pf6N2E2_69 at 111.554 kb on - strand, within Pf6N2E2_69 at 111.554 kb on - strand, within Pf6N2E2_69 at 111.554 kb on - strand, within Pf6N2E2_69 at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.159 kb on + strand at 112.160 kb on - strand at 112.160 kb on - strand at 112.282 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.284 kb on + strand at 112.285 kb on - strand at 112.285 kb on - strand at 112.285 kb on - strand at 112.569 kb on + strand at 112.569 kb on + strand at 112.569 kb on + strand at 112.570 kb on - strand at 112.570 kb on - strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.571 kb on + strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.572 kb on - strand at 112.589 kb on - strand at 112.589 kb on - strand at 112.603 kb on + strand at 112.620 kb on + strand at 112.620 kb on + strand at 112.621 kb on - strand at 112.682 kb on + strand at 112.683 kb on - strand at 112.991 kb on + strand, within Pf6N2E2_70 at 112.992 kb on - strand, within Pf6N2E2_70 at 112.992 kb on - strand, within Pf6N2E2_70 at 113.000 kb on + strand, within Pf6N2E2_70 at 113.000 kb on + strand, within Pf6N2E2_70 at 113.001 kb on - strand, within Pf6N2E2_70
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 109,084 - Pf6N2E2_68 0.62 -0.3 109,084 - Pf6N2E2_68 0.62 +0.1 109,195 - Pf6N2E2_68 0.73 -0.6 109,333 + Pf6N2E2_68 0.87 +0.1 109,333 + Pf6N2E2_68 0.87 +0.8 109,334 - Pf6N2E2_68 0.87 -0.2 109,334 - Pf6N2E2_68 0.87 +0.8 109,334 - Pf6N2E2_68 0.87 -1.5 109,376 + +0.2 109,376 + +0.2 109,376 + +0.2 109,376 + +0.4 109,376 + +0.7 109,377 - -0.8 109,377 - -0.6 109,377 - -0.6 109,380 + -0.2 109,380 + +0.3 109,380 + +0.2 109,381 - -1.1 109,381 - -0.8 109,381 - +0.5 109,381 - -0.8 109,398 + +0.2 109,451 + -0.0 109,465 + +0.2 109,472 + +0.2 109,472 + -0.6 109,500 + +0.2 109,511 + +0.7 109,511 + +0.5 109,529 + +0.4 109,530 - -2.0 109,618 - -1.0 109,634 + +0.7 109,634 + +1.1 109,634 + +0.5 109,634 + +1.4 109,678 - -1.4 109,703 + -0.8 109,703 + -1.2 109,704 - -0.0 109,704 - -0.9 109,709 + -0.2 109,710 - -2.0 110,051 - Pf6N2E2_69 0.14 -3.6 110,057 + Pf6N2E2_69 0.15 -1.9 110,057 + Pf6N2E2_69 0.15 -0.6 110,057 + Pf6N2E2_69 0.15 -2.3 110,058 - Pf6N2E2_69 0.15 +0.2 110,058 - Pf6N2E2_69 0.15 -1.1 110,058 - Pf6N2E2_69 0.15 -0.6 110,164 + Pf6N2E2_69 0.19 -0.4 110,164 + Pf6N2E2_69 0.19 -0.3 110,165 - Pf6N2E2_69 0.19 -0.9 110,165 - Pf6N2E2_69 0.19 -3.9 110,165 - Pf6N2E2_69 0.19 -1.3 110,905 + Pf6N2E2_69 0.47 -1.2 110,905 + Pf6N2E2_69 0.47 -1.4 110,905 + Pf6N2E2_69 0.47 -1.1 111,382 + Pf6N2E2_69 0.66 -0.1 111,383 - +2.4 111,553 + Pf6N2E2_69 0.72 -0.7 111,553 + Pf6N2E2_69 0.72 +0.8 111,554 - Pf6N2E2_69 0.72 -0.1 111,554 - Pf6N2E2_69 0.72 -0.6 111,554 - Pf6N2E2_69 0.72 +0.5 112,159 + +0.4 112,159 + -0.1 112,159 + +0.6 112,159 + -0.0 112,159 + +0.9 112,159 + -0.6 112,159 + +0.0 112,160 - +0.0 112,160 - -0.2 112,282 + -0.3 112,284 + -0.0 112,284 + +1.3 112,284 + +0.4 112,284 + -0.2 112,284 + +1.0 112,284 + -2.6 112,285 - -0.1 112,285 - -0.8 112,285 - +0.7 112,569 + -0.1 112,569 + +0.2 112,569 + -0.3 112,570 - +0.4 112,570 - +0.6 112,571 + +0.3 112,571 + -0.6 112,571 + +0.9 112,571 + +0.4 112,571 + +0.3 112,571 + +0.0 112,571 + -0.3 112,572 - +0.8 112,572 - +0.8 112,572 - +0.3 112,572 - +0.3 112,589 - -0.0 112,589 - -0.2 112,603 + +0.8 112,620 + -0.0 112,620 + -0.6 112,621 - -0.8 112,682 + +1.8 112,683 - -1.3 112,991 + Pf6N2E2_70 0.45 +0.6 112,992 - Pf6N2E2_70 0.45 -2.0 112,992 - Pf6N2E2_70 0.45 -1.5 113,000 + Pf6N2E2_70 0.46 -0.4 113,000 + Pf6N2E2_70 0.46 -1.1 113,001 - Pf6N2E2_70 0.46 +0.6
Or see this region's nucleotide sequence