Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5428 and Pf6N2E2_5429 are separated by 49 nucleotides Pf6N2E2_5429 and Pf6N2E2_5430 are separated by 204 nucleotides Pf6N2E2_5430 and Pf6N2E2_5431 are separated by 147 nucleotides Pf6N2E2_5431 and Pf6N2E2_5432 overlap by 45 nucleotides
Pf6N2E2_5428 - DNA recombination and repair protein RecO, at 6,233,476 to 6,234,165
_5428
Pf6N2E2_5429 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 6,234,215 to 6,234,958
_5429
Pf6N2E2_5430 - Transglycosylase, Slt family, at 6,235,163 to 6,236,500
_5430
Pf6N2E2_5431 - hypothetical protein, at 6,236,648 to 6,236,956
_5431
Pf6N2E2_5432 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 6,236,912 to 6,240,808
_5432
Position (kb)
6235
6236
6237 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 6234.174 kb on + strand at 6234.174 kb on + strand at 6234.175 kb on - strand at 6234.190 kb on + strand at 6234.190 kb on + strand at 6234.190 kb on + strand at 6234.985 kb on + strand at 6234.985 kb on + strand at 6234.985 kb on + strand at 6234.986 kb on - strand at 6235.082 kb on + strand at 6235.082 kb on + strand at 6235.083 kb on - strand at 6235.083 kb on - strand at 6235.156 kb on + strand at 6235.156 kb on + strand at 6235.156 kb on + strand at 6235.156 kb on + strand at 6235.157 kb on - strand at 6235.157 kb on - strand at 6235.281 kb on + strand at 6235.354 kb on - strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.473 kb on + strand, within Pf6N2E2_5430 at 6235.474 kb on - strand, within Pf6N2E2_5430 at 6235.474 kb on - strand, within Pf6N2E2_5430 at 6235.474 kb on - strand, within Pf6N2E2_5430 at 6235.539 kb on + strand, within Pf6N2E2_5430 at 6235.539 kb on + strand, within Pf6N2E2_5430 at 6235.540 kb on - strand, within Pf6N2E2_5430 at 6235.540 kb on - strand, within Pf6N2E2_5430 at 6235.549 kb on - strand, within Pf6N2E2_5430 at 6235.738 kb on - strand, within Pf6N2E2_5430 at 6235.759 kb on - strand, within Pf6N2E2_5430 at 6235.810 kb on - strand, within Pf6N2E2_5430 at 6235.887 kb on + strand, within Pf6N2E2_5430 at 6235.887 kb on + strand, within Pf6N2E2_5430 at 6235.888 kb on - strand, within Pf6N2E2_5430 at 6235.992 kb on + strand, within Pf6N2E2_5430 at 6235.992 kb on + strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6235.993 kb on - strand, within Pf6N2E2_5430 at 6236.063 kb on + strand, within Pf6N2E2_5430 at 6236.064 kb on - strand, within Pf6N2E2_5430 at 6236.064 kb on - strand, within Pf6N2E2_5430 at 6236.100 kb on + strand, within Pf6N2E2_5430 at 6236.101 kb on - strand, within Pf6N2E2_5430 at 6236.125 kb on + strand, within Pf6N2E2_5430 at 6236.253 kb on + strand, within Pf6N2E2_5430 at 6236.253 kb on + strand, within Pf6N2E2_5430 at 6236.253 kb on + strand, within Pf6N2E2_5430 at 6236.427 kb on + strand at 6236.463 kb on + strand at 6236.463 kb on + strand at 6236.463 kb on + strand at 6236.463 kb on + strand at 6236.464 kb on - strand at 6236.464 kb on - strand at 6236.464 kb on - strand at 6236.464 kb on - strand at 6236.657 kb on + strand at 6236.657 kb on + strand at 6236.658 kb on - strand at 6236.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 6,234,174 + +0.3 6,234,174 + +0.1 6,234,175 - -0.7 6,234,190 + +0.2 6,234,190 + +0.1 6,234,190 + +0.1 6,234,985 + -0.5 6,234,985 + -0.5 6,234,985 + +0.3 6,234,986 - +1.1 6,235,082 + +0.3 6,235,082 + -0.3 6,235,083 - -0.6 6,235,083 - +0.4 6,235,156 + -0.7 6,235,156 + +0.8 6,235,156 + -0.2 6,235,156 + -0.4 6,235,157 - -0.8 6,235,157 - +0.1 6,235,281 + -3.6 6,235,354 - Pf6N2E2_5430 0.14 -1.4 6,235,473 + Pf6N2E2_5430 0.23 -3.9 6,235,473 + Pf6N2E2_5430 0.23 -3.2 6,235,473 + Pf6N2E2_5430 0.23 -2.1 6,235,473 + Pf6N2E2_5430 0.23 -2.2 6,235,474 - Pf6N2E2_5430 0.23 -1.9 6,235,474 - Pf6N2E2_5430 0.23 -3.8 6,235,474 - Pf6N2E2_5430 0.23 -1.3 6,235,539 + Pf6N2E2_5430 0.28 -4.4 6,235,539 + Pf6N2E2_5430 0.28 -5.3 6,235,540 - Pf6N2E2_5430 0.28 -0.2 6,235,540 - Pf6N2E2_5430 0.28 -3.6 6,235,549 - Pf6N2E2_5430 0.29 -1.3 6,235,738 - Pf6N2E2_5430 0.43 -4.1 6,235,759 - Pf6N2E2_5430 0.45 -3.2 6,235,810 - Pf6N2E2_5430 0.48 -4.6 6,235,887 + Pf6N2E2_5430 0.54 -3.4 6,235,887 + Pf6N2E2_5430 0.54 -1.3 6,235,888 - Pf6N2E2_5430 0.54 -3.0 6,235,992 + Pf6N2E2_5430 0.62 -3.9 6,235,992 + Pf6N2E2_5430 0.62 -3.0 6,235,993 - Pf6N2E2_5430 0.62 -2.2 6,235,993 - Pf6N2E2_5430 0.62 -2.7 6,235,993 - Pf6N2E2_5430 0.62 -2.9 6,235,993 - Pf6N2E2_5430 0.62 -2.5 6,235,993 - Pf6N2E2_5430 0.62 -3.1 6,236,063 + Pf6N2E2_5430 0.67 -2.9 6,236,064 - Pf6N2E2_5430 0.67 -2.4 6,236,064 - Pf6N2E2_5430 0.67 -2.7 6,236,100 + Pf6N2E2_5430 0.70 -2.2 6,236,101 - Pf6N2E2_5430 0.70 -2.7 6,236,125 + Pf6N2E2_5430 0.72 -2.8 6,236,253 + Pf6N2E2_5430 0.81 -3.1 6,236,253 + Pf6N2E2_5430 0.81 -2.1 6,236,253 + Pf6N2E2_5430 0.81 -1.5 6,236,427 + -2.0 6,236,463 + -2.7 6,236,463 + -2.6 6,236,463 + -2.2 6,236,463 + -4.5 6,236,464 - -0.4 6,236,464 - -2.6 6,236,464 - -2.4 6,236,464 - -5.3 6,236,657 + -2.0 6,236,657 + -0.9 6,236,658 - -0.9 6,236,668 + -0.2
Or see this region's nucleotide sequence