Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5171 and Pf6N2E2_5172 are separated by 16 nucleotides Pf6N2E2_5172 and Pf6N2E2_5173 overlap by 1 nucleotides Pf6N2E2_5173 and Pf6N2E2_5174 are separated by 6 nucleotides
Pf6N2E2_5171 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 5,959,155 to 5,961,371
_5171
Pf6N2E2_5172 - FOG: TPR repeat, at 5,961,388 to 5,962,107
_5172
Pf6N2E2_5173 - Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C), at 5,962,107 to 5,962,436
_5173
Pf6N2E2_5174 - Glycosyltransferase, at 5,962,443 to 5,962,859
_5174
Position (kb)
5961
5962
5963 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5960.469 kb on + strand, within Pf6N2E2_5171 at 5960.469 kb on + strand, within Pf6N2E2_5171 at 5960.499 kb on + strand, within Pf6N2E2_5171 at 5960.499 kb on + strand, within Pf6N2E2_5171 at 5960.500 kb on - strand, within Pf6N2E2_5171 at 5960.500 kb on - strand, within Pf6N2E2_5171 at 5960.577 kb on + strand, within Pf6N2E2_5171 at 5960.577 kb on + strand, within Pf6N2E2_5171 at 5960.577 kb on + strand, within Pf6N2E2_5171 at 5960.577 kb on + strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.578 kb on - strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.631 kb on + strand, within Pf6N2E2_5171 at 5960.632 kb on - strand, within Pf6N2E2_5171 at 5960.632 kb on - strand, within Pf6N2E2_5171 at 5960.680 kb on + strand, within Pf6N2E2_5171 at 5960.759 kb on - strand, within Pf6N2E2_5171 at 5960.784 kb on + strand, within Pf6N2E2_5171 at 5960.784 kb on + strand, within Pf6N2E2_5171 at 5960.784 kb on + strand, within Pf6N2E2_5171 at 5960.784 kb on + strand, within Pf6N2E2_5171 at 5960.784 kb on + strand, within Pf6N2E2_5171 at 5960.785 kb on - strand, within Pf6N2E2_5171 at 5960.813 kb on + strand, within Pf6N2E2_5171 at 5960.814 kb on - strand, within Pf6N2E2_5171 at 5960.970 kb on + strand, within Pf6N2E2_5171 at 5960.970 kb on + strand, within Pf6N2E2_5171 at 5960.970 kb on + strand, within Pf6N2E2_5171 at 5960.970 kb on + strand, within Pf6N2E2_5171 at 5960.970 kb on + strand, within Pf6N2E2_5171 at 5960.971 kb on - strand, within Pf6N2E2_5171 at 5961.276 kb on + strand at 5961.276 kb on + strand at 5961.276 kb on + strand at 5961.277 kb on - strand at 5961.277 kb on - strand at 5961.278 kb on + strand at 5961.279 kb on - strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.369 kb on + strand at 5961.370 kb on - strand at 5961.370 kb on - strand at 5961.444 kb on + strand at 5961.444 kb on + strand at 5961.445 kb on - strand at 5961.449 kb on + strand at 5961.449 kb on + strand at 5961.449 kb on + strand at 5961.715 kb on + strand, within Pf6N2E2_5172 at 5961.715 kb on + strand, within Pf6N2E2_5172 at 5961.715 kb on + strand, within Pf6N2E2_5172 at 5961.716 kb on - strand, within Pf6N2E2_5172 at 5961.716 kb on - strand, within Pf6N2E2_5172 at 5961.716 kb on - strand, within Pf6N2E2_5172 at 5962.293 kb on - strand, within Pf6N2E2_5173 at 5962.356 kb on + strand, within Pf6N2E2_5173 at 5962.356 kb on + strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.357 kb on - strand, within Pf6N2E2_5173 at 5962.542 kb on + strand, within Pf6N2E2_5174 at 5962.543 kb on - strand, within Pf6N2E2_5174 at 5962.602 kb on + strand, within Pf6N2E2_5174 at 5962.603 kb on - strand, within Pf6N2E2_5174 at 5962.603 kb on - strand, within Pf6N2E2_5174 at 5962.795 kb on + strand, within Pf6N2E2_5174 at 5962.916 kb on + strand at 5962.946 kb on + strand at 5962.947 kb on - strand at 5962.947 kb on - strand at 5962.964 kb on + strand at 5962.964 kb on + strand at 5962.964 kb on + strand at 5962.965 kb on - strand at 5963.015 kb on - strand at 5963.078 kb on + strand at 5963.079 kb on - strand at 5963.079 kb on - strand at 5963.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 5,960,469 + Pf6N2E2_5171 0.59 +0.3 5,960,469 + Pf6N2E2_5171 0.59 +1.7 5,960,499 + Pf6N2E2_5171 0.61 +0.2 5,960,499 + Pf6N2E2_5171 0.61 -0.8 5,960,500 - Pf6N2E2_5171 0.61 +0.4 5,960,500 - Pf6N2E2_5171 0.61 +0.3 5,960,577 + Pf6N2E2_5171 0.64 -0.1 5,960,577 + Pf6N2E2_5171 0.64 +0.3 5,960,577 + Pf6N2E2_5171 0.64 -0.1 5,960,577 + Pf6N2E2_5171 0.64 +0.1 5,960,578 - Pf6N2E2_5171 0.64 -0.0 5,960,578 - Pf6N2E2_5171 0.64 +0.4 5,960,578 - Pf6N2E2_5171 0.64 -1.0 5,960,578 - Pf6N2E2_5171 0.64 -1.3 5,960,578 - Pf6N2E2_5171 0.64 -0.5 5,960,578 - Pf6N2E2_5171 0.64 +0.3 5,960,578 - Pf6N2E2_5171 0.64 -0.2 5,960,578 - Pf6N2E2_5171 0.64 -0.7 5,960,578 - Pf6N2E2_5171 0.64 -1.1 5,960,578 - Pf6N2E2_5171 0.64 +0.7 5,960,631 + Pf6N2E2_5171 0.67 -0.3 5,960,631 + Pf6N2E2_5171 0.67 -0.5 5,960,631 + Pf6N2E2_5171 0.67 -0.4 5,960,631 + Pf6N2E2_5171 0.67 -0.4 5,960,631 + Pf6N2E2_5171 0.67 -1.3 5,960,631 + Pf6N2E2_5171 0.67 +0.2 5,960,631 + Pf6N2E2_5171 0.67 -0.0 5,960,631 + Pf6N2E2_5171 0.67 -0.1 5,960,631 + Pf6N2E2_5171 0.67 -0.4 5,960,632 - Pf6N2E2_5171 0.67 -0.1 5,960,632 - Pf6N2E2_5171 0.67 +0.0 5,960,680 + Pf6N2E2_5171 0.69 +0.1 5,960,759 - Pf6N2E2_5171 0.72 -1.0 5,960,784 + Pf6N2E2_5171 0.73 -0.5 5,960,784 + Pf6N2E2_5171 0.73 -0.9 5,960,784 + Pf6N2E2_5171 0.73 -0.8 5,960,784 + Pf6N2E2_5171 0.73 -0.7 5,960,784 + Pf6N2E2_5171 0.73 +0.7 5,960,785 - Pf6N2E2_5171 0.74 +0.5 5,960,813 + Pf6N2E2_5171 0.75 +0.2 5,960,814 - Pf6N2E2_5171 0.75 -0.7 5,960,970 + Pf6N2E2_5171 0.82 -0.0 5,960,970 + Pf6N2E2_5171 0.82 -1.2 5,960,970 + Pf6N2E2_5171 0.82 -0.3 5,960,970 + Pf6N2E2_5171 0.82 +0.1 5,960,970 + Pf6N2E2_5171 0.82 -1.4 5,960,971 - Pf6N2E2_5171 0.82 -1.2 5,961,276 + -0.1 5,961,276 + -0.4 5,961,276 + -3.3 5,961,277 - -0.1 5,961,277 - +0.6 5,961,278 + +0.6 5,961,279 - -1.1 5,961,369 + -0.4 5,961,369 + -0.1 5,961,369 + +0.8 5,961,369 + +0.8 5,961,369 + -0.3 5,961,370 - -0.7 5,961,370 - -0.2 5,961,444 + -0.9 5,961,444 + +0.1 5,961,445 - +0.1 5,961,449 + -1.2 5,961,449 + +0.3 5,961,449 + +0.5 5,961,715 + Pf6N2E2_5172 0.45 -0.9 5,961,715 + Pf6N2E2_5172 0.45 -1.0 5,961,715 + Pf6N2E2_5172 0.45 -0.5 5,961,716 - Pf6N2E2_5172 0.46 -1.7 5,961,716 - Pf6N2E2_5172 0.46 -0.4 5,961,716 - Pf6N2E2_5172 0.46 -0.5 5,962,293 - Pf6N2E2_5173 0.56 +1.1 5,962,356 + Pf6N2E2_5173 0.75 -0.1 5,962,356 + Pf6N2E2_5173 0.75 +0.3 5,962,357 - Pf6N2E2_5173 0.76 +0.3 5,962,357 - Pf6N2E2_5173 0.76 +0.3 5,962,357 - Pf6N2E2_5173 0.76 +0.3 5,962,357 - Pf6N2E2_5173 0.76 +0.2 5,962,542 + Pf6N2E2_5174 0.24 +0.2 5,962,543 - Pf6N2E2_5174 0.24 -0.4 5,962,602 + Pf6N2E2_5174 0.38 +0.5 5,962,603 - Pf6N2E2_5174 0.38 +1.0 5,962,603 - Pf6N2E2_5174 0.38 +0.4 5,962,795 + Pf6N2E2_5174 0.84 +0.2 5,962,916 + -1.7 5,962,946 + -2.1 5,962,947 - +0.3 5,962,947 - -0.4 5,962,964 + +0.6 5,962,964 + -0.3 5,962,964 + +0.1 5,962,965 - +0.6 5,963,015 - +0.6 5,963,078 + -0.4 5,963,079 - +0.3 5,963,079 - +0.4 5,963,079 - -0.8
Or see this region's nucleotide sequence