Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4560 and Pf6N2E2_4561 are separated by 86 nucleotides Pf6N2E2_4561 and Pf6N2E2_4562 are separated by 144 nucleotides Pf6N2E2_4562 and Pf6N2E2_4563 are separated by 242 nucleotides
Pf6N2E2_4560 - probable integral membrane protein NMA1899, at 5,288,951 to 5,290,318
_4560
Pf6N2E2_4561 - Dihydrofolate reductase (EC 1.5.1.3), at 5,290,405 to 5,290,917
_4561
Pf6N2E2_4562 - L-cystine uptake protein TcyP, at 5,291,062 to 5,292,453
_4562
Pf6N2E2_4563 - Phosphoserine phosphatase (EC 3.1.3.3), at 5,292,696 to 5,293,751
_4563
Position (kb)
5291
5292
5293 Strain fitness (log2 ratio)
-1
0
1
2 at 5290.202 kb on + strand at 5290.280 kb on + strand at 5290.310 kb on + strand at 5290.310 kb on + strand at 5290.353 kb on - strand at 5290.392 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.926 kb on + strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5290.927 kb on - strand at 5291.071 kb on + strand at 5291.651 kb on + strand, within Pf6N2E2_4562 at 5291.651 kb on + strand, within Pf6N2E2_4562 at 5291.994 kb on - strand, within Pf6N2E2_4562 at 5292.303 kb on - strand, within Pf6N2E2_4562 at 5292.303 kb on - strand, within Pf6N2E2_4562 at 5292.341 kb on - strand at 5292.341 kb on - strand at 5292.514 kb on + strand at 5292.515 kb on - strand at 5292.515 kb on - strand at 5292.526 kb on - strand at 5292.530 kb on - strand at 5292.537 kb on + strand at 5292.555 kb on + strand at 5292.555 kb on + strand at 5292.556 kb on - strand at 5292.595 kb on + strand at 5292.837 kb on - strand, within Pf6N2E2_4563 at 5292.840 kb on + strand, within Pf6N2E2_4563 at 5292.840 kb on + strand, within Pf6N2E2_4563 at 5292.840 kb on + strand, within Pf6N2E2_4563 at 5292.840 kb on + strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.841 kb on - strand, within Pf6N2E2_4563 at 5292.870 kb on + strand, within Pf6N2E2_4563 at 5292.871 kb on - strand, within Pf6N2E2_4563 at 5293.005 kb on + strand, within Pf6N2E2_4563 at 5293.005 kb on + strand, within Pf6N2E2_4563 at 5293.005 kb on + strand, within Pf6N2E2_4563 at 5293.006 kb on - strand, within Pf6N2E2_4563 at 5293.006 kb on - strand, within Pf6N2E2_4563 at 5293.008 kb on + strand, within Pf6N2E2_4563 at 5293.008 kb on + strand, within Pf6N2E2_4563 at 5293.009 kb on - strand, within Pf6N2E2_4563 at 5293.009 kb on - strand, within Pf6N2E2_4563 at 5293.009 kb on - strand, within Pf6N2E2_4563 at 5293.044 kb on + strand, within Pf6N2E2_4563 at 5293.099 kb on - strand, within Pf6N2E2_4563 at 5293.099 kb on - strand, within Pf6N2E2_4563 at 5293.099 kb on - strand, within Pf6N2E2_4563 at 5293.099 kb on - strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.143 kb on + strand, within Pf6N2E2_4563 at 5293.144 kb on - strand, within Pf6N2E2_4563 at 5293.144 kb on - strand, within Pf6N2E2_4563 at 5293.144 kb on - strand, within Pf6N2E2_4563 at 5293.144 kb on - strand, within Pf6N2E2_4563 at 5293.144 kb on - strand, within Pf6N2E2_4563 at 5293.146 kb on + strand, within Pf6N2E2_4563 at 5293.146 kb on + strand, within Pf6N2E2_4563 at 5293.146 kb on + strand, within Pf6N2E2_4563 at 5293.146 kb on + strand, within Pf6N2E2_4563 at 5293.146 kb on + strand, within Pf6N2E2_4563 at 5293.146 kb on + strand, within Pf6N2E2_4563 at 5293.147 kb on - strand, within Pf6N2E2_4563 at 5293.147 kb on - strand, within Pf6N2E2_4563 at 5293.147 kb on - strand, within Pf6N2E2_4563 at 5293.170 kb on + strand, within Pf6N2E2_4563 at 5293.170 kb on + strand, within Pf6N2E2_4563 at 5293.170 kb on + strand, within Pf6N2E2_4563 at 5293.171 kb on - strand, within Pf6N2E2_4563 at 5293.215 kb on + strand, within Pf6N2E2_4563 at 5293.215 kb on + strand, within Pf6N2E2_4563 at 5293.215 kb on + strand, within Pf6N2E2_4563 at 5293.215 kb on + strand, within Pf6N2E2_4563 at 5293.216 kb on - strand, within Pf6N2E2_4563 at 5293.216 kb on - strand, within Pf6N2E2_4563 at 5293.216 kb on - strand, within Pf6N2E2_4563 at 5293.216 kb on - strand, within Pf6N2E2_4563 at 5293.216 kb on - strand, within Pf6N2E2_4563 at 5293.216 kb on - strand, within Pf6N2E2_4563 at 5293.248 kb on + strand, within Pf6N2E2_4563 at 5293.248 kb on + strand, within Pf6N2E2_4563 at 5293.248 kb on + strand, within Pf6N2E2_4563 at 5293.248 kb on + strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.249 kb on - strand, within Pf6N2E2_4563 at 5293.311 kb on + strand, within Pf6N2E2_4563 at 5293.312 kb on - strand, within Pf6N2E2_4563 at 5293.413 kb on + strand, within Pf6N2E2_4563 at 5293.414 kb on - strand, within Pf6N2E2_4563 at 5293.443 kb on + strand, within Pf6N2E2_4563 at 5293.444 kb on - strand, within Pf6N2E2_4563 at 5293.453 kb on - strand, within Pf6N2E2_4563 at 5293.453 kb on - strand, within Pf6N2E2_4563
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 5,290,202 + +1.4 5,290,280 + +0.0 5,290,310 + -0.1 5,290,310 + +0.3 5,290,353 - +0.6 5,290,392 + -0.4 5,290,926 + -0.8 5,290,926 + +0.4 5,290,926 + +0.1 5,290,926 + -0.4 5,290,926 + -0.7 5,290,926 + -0.5 5,290,927 - +0.6 5,290,927 - +0.2 5,290,927 - +0.7 5,290,927 - +0.4 5,290,927 - +0.0 5,291,071 + +0.0 5,291,651 + Pf6N2E2_4562 0.42 -0.4 5,291,651 + Pf6N2E2_4562 0.42 -1.0 5,291,994 - Pf6N2E2_4562 0.67 -0.9 5,292,303 - Pf6N2E2_4562 0.89 -0.4 5,292,303 - Pf6N2E2_4562 0.89 -0.2 5,292,341 - +0.4 5,292,341 - -0.5 5,292,514 + -0.6 5,292,515 - +0.2 5,292,515 - +0.7 5,292,526 - -0.1 5,292,530 - -0.9 5,292,537 + -1.0 5,292,555 + -0.1 5,292,555 + +1.2 5,292,556 - -0.2 5,292,595 + +0.6 5,292,837 - Pf6N2E2_4563 0.13 -0.5 5,292,840 + Pf6N2E2_4563 0.14 +0.6 5,292,840 + Pf6N2E2_4563 0.14 +0.5 5,292,840 + Pf6N2E2_4563 0.14 +0.2 5,292,840 + Pf6N2E2_4563 0.14 -0.2 5,292,841 - Pf6N2E2_4563 0.14 -0.2 5,292,841 - Pf6N2E2_4563 0.14 -0.3 5,292,841 - Pf6N2E2_4563 0.14 +0.5 5,292,841 - Pf6N2E2_4563 0.14 -0.7 5,292,841 - Pf6N2E2_4563 0.14 +1.0 5,292,841 - Pf6N2E2_4563 0.14 +0.5 5,292,841 - Pf6N2E2_4563 0.14 +0.2 5,292,870 + Pf6N2E2_4563 0.16 +0.9 5,292,871 - Pf6N2E2_4563 0.17 +0.3 5,293,005 + Pf6N2E2_4563 0.29 -1.0 5,293,005 + Pf6N2E2_4563 0.29 +0.5 5,293,005 + Pf6N2E2_4563 0.29 -0.0 5,293,006 - Pf6N2E2_4563 0.29 +0.2 5,293,006 - Pf6N2E2_4563 0.29 +0.2 5,293,008 + Pf6N2E2_4563 0.30 +0.9 5,293,008 + Pf6N2E2_4563 0.30 +0.9 5,293,009 - Pf6N2E2_4563 0.30 +0.2 5,293,009 - Pf6N2E2_4563 0.30 +0.2 5,293,009 - Pf6N2E2_4563 0.30 -0.6 5,293,044 + Pf6N2E2_4563 0.33 -1.1 5,293,099 - Pf6N2E2_4563 0.38 -0.6 5,293,099 - Pf6N2E2_4563 0.38 -0.9 5,293,099 - Pf6N2E2_4563 0.38 +0.7 5,293,099 - Pf6N2E2_4563 0.38 +0.5 5,293,143 + Pf6N2E2_4563 0.42 +2.0 5,293,143 + Pf6N2E2_4563 0.42 +0.1 5,293,143 + Pf6N2E2_4563 0.42 -0.2 5,293,143 + Pf6N2E2_4563 0.42 +1.2 5,293,143 + Pf6N2E2_4563 0.42 +0.3 5,293,143 + Pf6N2E2_4563 0.42 -0.2 5,293,143 + Pf6N2E2_4563 0.42 -0.2 5,293,143 + Pf6N2E2_4563 0.42 +0.6 5,293,144 - Pf6N2E2_4563 0.42 -0.1 5,293,144 - Pf6N2E2_4563 0.42 -0.2 5,293,144 - Pf6N2E2_4563 0.42 -1.2 5,293,144 - Pf6N2E2_4563 0.42 +1.7 5,293,144 - Pf6N2E2_4563 0.42 -0.2 5,293,146 + Pf6N2E2_4563 0.43 -0.5 5,293,146 + Pf6N2E2_4563 0.43 +0.1 5,293,146 + Pf6N2E2_4563 0.43 +0.7 5,293,146 + Pf6N2E2_4563 0.43 +0.6 5,293,146 + Pf6N2E2_4563 0.43 -0.1 5,293,146 + Pf6N2E2_4563 0.43 +0.9 5,293,147 - Pf6N2E2_4563 0.43 +0.5 5,293,147 - Pf6N2E2_4563 0.43 +0.2 5,293,147 - Pf6N2E2_4563 0.43 +0.6 5,293,170 + Pf6N2E2_4563 0.45 +0.5 5,293,170 + Pf6N2E2_4563 0.45 -0.4 5,293,170 + Pf6N2E2_4563 0.45 -1.5 5,293,171 - Pf6N2E2_4563 0.45 +0.2 5,293,215 + Pf6N2E2_4563 0.49 +0.7 5,293,215 + Pf6N2E2_4563 0.49 +0.8 5,293,215 + Pf6N2E2_4563 0.49 +0.6 5,293,215 + Pf6N2E2_4563 0.49 +0.3 5,293,216 - Pf6N2E2_4563 0.49 +0.4 5,293,216 - Pf6N2E2_4563 0.49 +0.3 5,293,216 - Pf6N2E2_4563 0.49 -0.4 5,293,216 - Pf6N2E2_4563 0.49 -0.5 5,293,216 - Pf6N2E2_4563 0.49 -0.4 5,293,216 - Pf6N2E2_4563 0.49 +1.8 5,293,248 + Pf6N2E2_4563 0.52 +0.3 5,293,248 + Pf6N2E2_4563 0.52 -1.1 5,293,248 + Pf6N2E2_4563 0.52 +0.4 5,293,248 + Pf6N2E2_4563 0.52 +0.4 5,293,249 - Pf6N2E2_4563 0.52 -0.4 5,293,249 - Pf6N2E2_4563 0.52 +0.1 5,293,249 - Pf6N2E2_4563 0.52 +0.2 5,293,249 - Pf6N2E2_4563 0.52 -0.2 5,293,249 - Pf6N2E2_4563 0.52 +0.2 5,293,249 - Pf6N2E2_4563 0.52 +1.0 5,293,249 - Pf6N2E2_4563 0.52 +1.4 5,293,249 - Pf6N2E2_4563 0.52 +0.1 5,293,249 - Pf6N2E2_4563 0.52 +0.2 5,293,311 + Pf6N2E2_4563 0.58 -0.7 5,293,312 - Pf6N2E2_4563 0.58 -0.1 5,293,413 + Pf6N2E2_4563 0.68 +0.3 5,293,414 - Pf6N2E2_4563 0.68 +0.3 5,293,443 + Pf6N2E2_4563 0.71 -0.3 5,293,444 - Pf6N2E2_4563 0.71 -0.2 5,293,453 - Pf6N2E2_4563 0.72 +1.0 5,293,453 - Pf6N2E2_4563 0.72 +0.0
Or see this region's nucleotide sequence