Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4544 and Pf6N2E2_4545 are separated by 25 nucleotides Pf6N2E2_4545 and Pf6N2E2_4546 overlap by 14 nucleotides Pf6N2E2_4546 and Pf6N2E2_4547 are separated by 542 nucleotides
Pf6N2E2_4544 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 5,275,252 to 5,277,369
_4544
Pf6N2E2_4545 - hypothetical protein, at 5,277,395 to 5,277,832
_4545
Pf6N2E2_4546 - hypothetical protein, at 5,277,819 to 5,278,043
_4546
Pf6N2E2_4547 - Type III secretion cytoplasmic protein (YscL), at 5,278,586 to 5,279,179
_4547
Position (kb)
5277
5278 Strain fitness (log2 ratio)
-1
0
1
2 at 5276.465 kb on + strand, within Pf6N2E2_4544 at 5276.465 kb on + strand, within Pf6N2E2_4544 at 5276.466 kb on - strand, within Pf6N2E2_4544 at 5276.466 kb on - strand, within Pf6N2E2_4544 at 5276.750 kb on + strand, within Pf6N2E2_4544 at 5276.751 kb on - strand, within Pf6N2E2_4544 at 5276.861 kb on + strand, within Pf6N2E2_4544 at 5276.862 kb on - strand, within Pf6N2E2_4544 at 5276.862 kb on - strand, within Pf6N2E2_4544 at 5277.026 kb on + strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.148 kb on + strand, within Pf6N2E2_4544 at 5277.168 kb on + strand at 5277.169 kb on - strand at 5277.187 kb on + strand at 5277.219 kb on - strand at 5277.224 kb on + strand at 5277.224 kb on + strand at 5277.224 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.353 kb on - strand at 5277.377 kb on + strand at 5277.478 kb on + strand, within Pf6N2E2_4545 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.068 kb on + strand at 5278.068 kb on + strand at 5278.068 kb on + strand at 5278.090 kb on + strand at 5278.091 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.219 kb on - strand at 5278.219 kb on - strand at 5278.244 kb on + strand at 5278.244 kb on + strand at 5278.381 kb on - strand at 5278.381 kb on - strand at 5278.443 kb on + strand at 5278.445 kb on + strand at 5278.445 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.493 kb on + strand at 5278.494 kb on - strand at 5278.530 kb on + strand at 5278.531 kb on - strand at 5278.531 kb on - strand at 5278.531 kb on - strand at 5278.622 kb on + strand at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.656 kb on + strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.657 kb on - strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.660 kb on + strand, within Pf6N2E2_4547 at 5278.661 kb on - strand, within Pf6N2E2_4547 at 5278.661 kb on - strand, within Pf6N2E2_4547
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 5,276,465 + Pf6N2E2_4544 0.57 +0.4 5,276,465 + Pf6N2E2_4544 0.57 +0.5 5,276,466 - Pf6N2E2_4544 0.57 +0.3 5,276,466 - Pf6N2E2_4544 0.57 +0.6 5,276,750 + Pf6N2E2_4544 0.71 -0.5 5,276,751 - Pf6N2E2_4544 0.71 +0.2 5,276,861 + Pf6N2E2_4544 0.76 -0.2 5,276,862 - Pf6N2E2_4544 0.76 +0.8 5,276,862 - Pf6N2E2_4544 0.76 +0.6 5,277,026 + Pf6N2E2_4544 0.84 -1.0 5,277,027 - Pf6N2E2_4544 0.84 -0.2 5,277,027 - Pf6N2E2_4544 0.84 -0.1 5,277,027 - Pf6N2E2_4544 0.84 -0.7 5,277,041 + Pf6N2E2_4544 0.84 +0.1 5,277,041 + Pf6N2E2_4544 0.84 +0.7 5,277,041 + Pf6N2E2_4544 0.84 -0.6 5,277,041 + Pf6N2E2_4544 0.84 +1.1 5,277,041 + Pf6N2E2_4544 0.84 +0.4 5,277,041 + Pf6N2E2_4544 0.84 +1.0 5,277,041 + Pf6N2E2_4544 0.84 +0.8 5,277,042 - Pf6N2E2_4544 0.85 -0.4 5,277,042 - Pf6N2E2_4544 0.85 -0.6 5,277,042 - Pf6N2E2_4544 0.85 +0.3 5,277,042 - Pf6N2E2_4544 0.85 -0.2 5,277,042 - Pf6N2E2_4544 0.85 +0.2 5,277,042 - Pf6N2E2_4544 0.85 +1.0 5,277,148 + Pf6N2E2_4544 0.90 -0.6 5,277,168 + +0.6 5,277,169 - +0.7 5,277,187 + +0.8 5,277,219 - +0.0 5,277,224 + +0.1 5,277,224 + +0.3 5,277,224 + +1.2 5,277,352 + +0.7 5,277,352 + +1.5 5,277,352 + -0.1 5,277,352 + -0.3 5,277,352 + +0.8 5,277,353 - -0.2 5,277,377 + -0.8 5,277,478 + Pf6N2E2_4545 0.19 -0.5 5,278,003 + Pf6N2E2_4546 0.82 +0.3 5,278,003 + Pf6N2E2_4546 0.82 +1.4 5,278,003 + Pf6N2E2_4546 0.82 -0.3 5,278,003 + Pf6N2E2_4546 0.82 -0.1 5,278,004 - Pf6N2E2_4546 0.82 -0.0 5,278,004 - Pf6N2E2_4546 0.82 +0.4 5,278,004 - Pf6N2E2_4546 0.82 +0.5 5,278,004 - Pf6N2E2_4546 0.82 +0.4 5,278,004 - Pf6N2E2_4546 0.82 +0.8 5,278,068 + -0.4 5,278,068 + +0.1 5,278,068 + -0.1 5,278,090 + +1.1 5,278,091 - +0.5 5,278,215 - +0.5 5,278,215 - -1.3 5,278,215 - +0.0 5,278,215 - +0.1 5,278,215 - -0.1 5,278,215 - +0.7 5,278,219 - +0.8 5,278,219 - +0.6 5,278,244 + +0.1 5,278,244 + -0.3 5,278,381 - -0.7 5,278,381 - +0.4 5,278,443 + -0.0 5,278,445 + -0.8 5,278,445 + +0.8 5,278,493 + +0.1 5,278,493 + +0.4 5,278,493 + +2.2 5,278,493 + +0.7 5,278,493 + -0.5 5,278,493 + +0.2 5,278,494 - -0.7 5,278,530 + +1.0 5,278,531 - -1.6 5,278,531 - -0.8 5,278,531 - -0.1 5,278,622 + +0.0 5,278,656 + Pf6N2E2_4547 0.12 -0.2 5,278,656 + Pf6N2E2_4547 0.12 -0.0 5,278,656 + Pf6N2E2_4547 0.12 +0.1 5,278,656 + Pf6N2E2_4547 0.12 +0.2 5,278,656 + Pf6N2E2_4547 0.12 +0.3 5,278,656 + Pf6N2E2_4547 0.12 -0.2 5,278,656 + Pf6N2E2_4547 0.12 +0.5 5,278,657 - Pf6N2E2_4547 0.12 +0.3 5,278,657 - Pf6N2E2_4547 0.12 -1.0 5,278,657 - Pf6N2E2_4547 0.12 -0.8 5,278,657 - Pf6N2E2_4547 0.12 -0.1 5,278,660 + Pf6N2E2_4547 0.12 -0.5 5,278,660 + Pf6N2E2_4547 0.12 +1.0 5,278,660 + Pf6N2E2_4547 0.12 -0.8 5,278,660 + Pf6N2E2_4547 0.12 +0.5 5,278,660 + Pf6N2E2_4547 0.12 +0.4 5,278,661 - Pf6N2E2_4547 0.13 +0.1 5,278,661 - Pf6N2E2_4547 0.13 -0.2
Or see this region's nucleotide sequence