Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4527

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4524 and Pf6N2E2_4525 overlap by 4 nucleotidesPf6N2E2_4525 and Pf6N2E2_4526 are separated by 62 nucleotidesPf6N2E2_4526 and Pf6N2E2_4527 overlap by 4 nucleotidesPf6N2E2_4527 and Pf6N2E2_4528 are separated by 226 nucleotidesPf6N2E2_4528 and Pf6N2E2_4529 overlap by 30 nucleotides Pf6N2E2_4524 - putative cytochrome P450 hydroxylase, at 5,259,871 to 5,261,088 _4524 Pf6N2E2_4525 - hypothetical protein, at 5,261,085 to 5,261,213 _4525 Pf6N2E2_4526 - Phosphinothricin N-acetyltransferase (EC 2.3.1.-), at 5,261,276 to 5,261,872 _4526 Pf6N2E2_4527 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,261,869 to 5,262,420 _4527 Pf6N2E2_4528 - hypothetical protein, at 5,262,647 to 5,262,760 _4528 Pf6N2E2_4529 - type III secretion protein HrpJ, at 5,262,731 to 5,263,858 _4529 Position (kb) 5261 5262 5263Strain fitness (log2 ratio) -2 -1 0 1at 5260.915 kb on + strand, within Pf6N2E2_4524at 5260.915 kb on + strand, within Pf6N2E2_4524at 5260.915 kb on + strand, within Pf6N2E2_4524at 5260.973 kb on + strandat 5260.973 kb on + strandat 5260.974 kb on - strandat 5260.974 kb on - strandat 5261.019 kb on - strandat 5261.030 kb on - strandat 5261.359 kb on - strand, within Pf6N2E2_4526at 5261.439 kb on - strand, within Pf6N2E2_4526at 5261.499 kb on + strand, within Pf6N2E2_4526at 5261.500 kb on - strand, within Pf6N2E2_4526at 5261.570 kb on + strand, within Pf6N2E2_4526at 5261.571 kb on - strand, within Pf6N2E2_4526at 5261.574 kb on + strand, within Pf6N2E2_4526at 5261.574 kb on + strand, within Pf6N2E2_4526at 5261.575 kb on - strand, within Pf6N2E2_4526at 5261.575 kb on - strand, within Pf6N2E2_4526at 5261.575 kb on - strand, within Pf6N2E2_4526at 5261.575 kb on - strand, within Pf6N2E2_4526at 5261.575 kb on - strand, within Pf6N2E2_4526at 5261.575 kb on - strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.673 kb on + strand, within Pf6N2E2_4526at 5261.674 kb on - strand, within Pf6N2E2_4526at 5261.674 kb on - strand, within Pf6N2E2_4526at 5261.674 kb on - strand, within Pf6N2E2_4526at 5261.674 kb on - strand, within Pf6N2E2_4526at 5261.674 kb on - strand, within Pf6N2E2_4526at 5261.758 kb on + strand, within Pf6N2E2_4526at 5261.758 kb on + strand, within Pf6N2E2_4526at 5261.759 kb on - strand, within Pf6N2E2_4526at 5261.786 kb on - strand, within Pf6N2E2_4526at 5261.786 kb on - strand, within Pf6N2E2_4526at 5261.888 kb on + strandat 5262.158 kb on + strand, within Pf6N2E2_4527at 5262.159 kb on - strand, within Pf6N2E2_4527at 5262.159 kb on - strand, within Pf6N2E2_4527at 5262.424 kb on + strandat 5262.424 kb on + strandat 5262.424 kb on + strandat 5262.424 kb on + strandat 5262.425 kb on - strandat 5262.425 kb on - strandat 5262.425 kb on - strandat 5262.425 kb on - strandat 5262.439 kb on - strandat 5262.514 kb on - strandat 5262.556 kb on + strandat 5262.738 kb on + strandat 5262.738 kb on + strandat 5262.739 kb on - strandat 5262.739 kb on - strandat 5262.739 kb on - strandat 5262.760 kb on + strandat 5262.892 kb on + strand, within Pf6N2E2_4529at 5262.893 kb on - strand, within Pf6N2E2_4529at 5262.893 kb on - strand, within Pf6N2E2_4529at 5263.197 kb on + strandat 5263.198 kb on - strand, within Pf6N2E2_4529at 5263.198 kb on - strand, within Pf6N2E2_4529at 5263.198 kb on - strand, within Pf6N2E2_4529at 5263.304 kb on + strand, within Pf6N2E2_4529at 5263.304 kb on + strand, within Pf6N2E2_4529at 5263.304 kb on + strand, within Pf6N2E2_4529at 5263.305 kb on - strand, within Pf6N2E2_4529at 5263.307 kb on + strand, within Pf6N2E2_4529at 5263.307 kb on + strand, within Pf6N2E2_4529at 5263.308 kb on - strand, within Pf6N2E2_4529at 5263.308 kb on - strand, within Pf6N2E2_4529at 5263.308 kb on - strand, within Pf6N2E2_4529

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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5,260,915 + Pf6N2E2_4524 0.86 +0.5
5,260,915 + Pf6N2E2_4524 0.86 -0.1
5,260,915 + Pf6N2E2_4524 0.86 -0.0
5,260,973 + -0.3
5,260,973 + +0.8
5,260,974 - -0.1
5,260,974 - -0.4
5,261,019 - +0.9
5,261,030 - -0.3
5,261,359 - Pf6N2E2_4526 0.14 +0.1
5,261,439 - Pf6N2E2_4526 0.27 -0.5
5,261,499 + Pf6N2E2_4526 0.37 +0.2
5,261,500 - Pf6N2E2_4526 0.38 -0.1
5,261,570 + Pf6N2E2_4526 0.49 -0.8
5,261,571 - Pf6N2E2_4526 0.49 +0.2
5,261,574 + Pf6N2E2_4526 0.50 -0.4
5,261,574 + Pf6N2E2_4526 0.50 -0.6
5,261,575 - Pf6N2E2_4526 0.50 -0.1
5,261,575 - Pf6N2E2_4526 0.50 +0.8
5,261,575 - Pf6N2E2_4526 0.50 +0.3
5,261,575 - Pf6N2E2_4526 0.50 -0.7
5,261,575 - Pf6N2E2_4526 0.50 +0.6
5,261,575 - Pf6N2E2_4526 0.50 +0.2
5,261,673 + Pf6N2E2_4526 0.66 -1.5
5,261,673 + Pf6N2E2_4526 0.66 +0.2
5,261,673 + Pf6N2E2_4526 0.66 +0.7
5,261,673 + Pf6N2E2_4526 0.66 +0.7
5,261,673 + Pf6N2E2_4526 0.66 -0.3
5,261,673 + Pf6N2E2_4526 0.66 -0.3
5,261,673 + Pf6N2E2_4526 0.66 +0.8
5,261,673 + Pf6N2E2_4526 0.66 -0.4
5,261,674 - Pf6N2E2_4526 0.67 +0.4
5,261,674 - Pf6N2E2_4526 0.67 -0.1
5,261,674 - Pf6N2E2_4526 0.67 -0.1
5,261,674 - Pf6N2E2_4526 0.67 -1.0
5,261,674 - Pf6N2E2_4526 0.67 -0.3
5,261,758 + Pf6N2E2_4526 0.81 -0.3
5,261,758 + Pf6N2E2_4526 0.81 +0.6
5,261,759 - Pf6N2E2_4526 0.81 -0.2
5,261,786 - Pf6N2E2_4526 0.85 +0.3
5,261,786 - Pf6N2E2_4526 0.85 +0.5
5,261,888 + +0.8
5,262,158 + Pf6N2E2_4527 0.52 -2.1
5,262,159 - Pf6N2E2_4527 0.53 -0.8
5,262,159 - Pf6N2E2_4527 0.53 -0.3
5,262,424 + +0.2
5,262,424 + +0.2
5,262,424 + +0.7
5,262,424 + -0.3
5,262,425 - -0.4
5,262,425 - -0.1
5,262,425 - -1.0
5,262,425 - +0.3
5,262,439 - -0.0
5,262,514 - -0.5
5,262,556 + -1.1
5,262,738 + +0.3
5,262,738 + +0.2
5,262,739 - +0.0
5,262,739 - +0.6
5,262,739 - -0.8
5,262,760 + -1.1
5,262,892 + Pf6N2E2_4529 0.14 -0.1
5,262,893 - Pf6N2E2_4529 0.14 +0.4
5,262,893 - Pf6N2E2_4529 0.14 -0.9
5,263,197 + +0.9
5,263,198 - Pf6N2E2_4529 0.41 -1.3
5,263,198 - Pf6N2E2_4529 0.41 +0.5
5,263,198 - Pf6N2E2_4529 0.41 -0.6
5,263,304 + Pf6N2E2_4529 0.51 -0.2
5,263,304 + Pf6N2E2_4529 0.51 +0.5
5,263,304 + Pf6N2E2_4529 0.51 +0.6
5,263,305 - Pf6N2E2_4529 0.51 +0.3
5,263,307 + Pf6N2E2_4529 0.51 +0.4
5,263,307 + Pf6N2E2_4529 0.51 -0.4
5,263,308 - Pf6N2E2_4529 0.51 -0.3
5,263,308 - Pf6N2E2_4529 0.51 +0.7
5,263,308 - Pf6N2E2_4529 0.51 +0.3

Or see this region's nucleotide sequence