Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4524 and Pf6N2E2_4525 overlap by 4 nucleotides Pf6N2E2_4525 and Pf6N2E2_4526 are separated by 62 nucleotides Pf6N2E2_4526 and Pf6N2E2_4527 overlap by 4 nucleotides Pf6N2E2_4527 and Pf6N2E2_4528 are separated by 226 nucleotides Pf6N2E2_4528 and Pf6N2E2_4529 overlap by 30 nucleotides
Pf6N2E2_4524 - putative cytochrome P450 hydroxylase, at 5,259,871 to 5,261,088
_4524
Pf6N2E2_4525 - hypothetical protein, at 5,261,085 to 5,261,213
_4525
Pf6N2E2_4526 - Phosphinothricin N-acetyltransferase (EC 2.3.1.-), at 5,261,276 to 5,261,872
_4526
Pf6N2E2_4527 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,261,869 to 5,262,420
_4527
Pf6N2E2_4528 - hypothetical protein, at 5,262,647 to 5,262,760
_4528
Pf6N2E2_4529 - type III secretion protein HrpJ, at 5,262,731 to 5,263,858
_4529
Position (kb)
5261
5262
5263 Strain fitness (log2 ratio)
-2
-1
0
1 at 5260.915 kb on + strand, within Pf6N2E2_4524 at 5260.915 kb on + strand, within Pf6N2E2_4524 at 5260.915 kb on + strand, within Pf6N2E2_4524 at 5260.973 kb on + strand at 5260.973 kb on + strand at 5260.974 kb on - strand at 5260.974 kb on - strand at 5261.019 kb on - strand at 5261.030 kb on - strand at 5261.359 kb on - strand, within Pf6N2E2_4526 at 5261.439 kb on - strand, within Pf6N2E2_4526 at 5261.499 kb on + strand, within Pf6N2E2_4526 at 5261.500 kb on - strand, within Pf6N2E2_4526 at 5261.570 kb on + strand, within Pf6N2E2_4526 at 5261.571 kb on - strand, within Pf6N2E2_4526 at 5261.574 kb on + strand, within Pf6N2E2_4526 at 5261.574 kb on + strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.575 kb on - strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.673 kb on + strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.674 kb on - strand, within Pf6N2E2_4526 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.758 kb on + strand, within Pf6N2E2_4526 at 5261.759 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.786 kb on - strand, within Pf6N2E2_4526 at 5261.888 kb on + strand at 5262.158 kb on + strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.159 kb on - strand, within Pf6N2E2_4527 at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.424 kb on + strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.425 kb on - strand at 5262.439 kb on - strand at 5262.514 kb on - strand at 5262.556 kb on + strand at 5262.738 kb on + strand at 5262.738 kb on + strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.739 kb on - strand at 5262.760 kb on + strand at 5262.892 kb on + strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5262.893 kb on - strand, within Pf6N2E2_4529 at 5263.197 kb on + strand at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.198 kb on - strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.304 kb on + strand, within Pf6N2E2_4529 at 5263.305 kb on - strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.307 kb on + strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529 at 5263.308 kb on - strand, within Pf6N2E2_4529
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 5,260,915 + Pf6N2E2_4524 0.86 +0.5 5,260,915 + Pf6N2E2_4524 0.86 -0.1 5,260,915 + Pf6N2E2_4524 0.86 -0.0 5,260,973 + -0.3 5,260,973 + +0.8 5,260,974 - -0.1 5,260,974 - -0.4 5,261,019 - +0.9 5,261,030 - -0.3 5,261,359 - Pf6N2E2_4526 0.14 +0.1 5,261,439 - Pf6N2E2_4526 0.27 -0.5 5,261,499 + Pf6N2E2_4526 0.37 +0.2 5,261,500 - Pf6N2E2_4526 0.38 -0.1 5,261,570 + Pf6N2E2_4526 0.49 -0.8 5,261,571 - Pf6N2E2_4526 0.49 +0.2 5,261,574 + Pf6N2E2_4526 0.50 -0.4 5,261,574 + Pf6N2E2_4526 0.50 -0.6 5,261,575 - Pf6N2E2_4526 0.50 -0.1 5,261,575 - Pf6N2E2_4526 0.50 +0.8 5,261,575 - Pf6N2E2_4526 0.50 +0.3 5,261,575 - Pf6N2E2_4526 0.50 -0.7 5,261,575 - Pf6N2E2_4526 0.50 +0.6 5,261,575 - Pf6N2E2_4526 0.50 +0.2 5,261,673 + Pf6N2E2_4526 0.66 -1.5 5,261,673 + Pf6N2E2_4526 0.66 +0.2 5,261,673 + Pf6N2E2_4526 0.66 +0.7 5,261,673 + Pf6N2E2_4526 0.66 +0.7 5,261,673 + Pf6N2E2_4526 0.66 -0.3 5,261,673 + Pf6N2E2_4526 0.66 -0.3 5,261,673 + Pf6N2E2_4526 0.66 +0.8 5,261,673 + Pf6N2E2_4526 0.66 -0.4 5,261,674 - Pf6N2E2_4526 0.67 +0.4 5,261,674 - Pf6N2E2_4526 0.67 -0.1 5,261,674 - Pf6N2E2_4526 0.67 -0.1 5,261,674 - Pf6N2E2_4526 0.67 -1.0 5,261,674 - Pf6N2E2_4526 0.67 -0.3 5,261,758 + Pf6N2E2_4526 0.81 -0.3 5,261,758 + Pf6N2E2_4526 0.81 +0.6 5,261,759 - Pf6N2E2_4526 0.81 -0.2 5,261,786 - Pf6N2E2_4526 0.85 +0.3 5,261,786 - Pf6N2E2_4526 0.85 +0.5 5,261,888 + +0.8 5,262,158 + Pf6N2E2_4527 0.52 -2.1 5,262,159 - Pf6N2E2_4527 0.53 -0.8 5,262,159 - Pf6N2E2_4527 0.53 -0.3 5,262,424 + +0.2 5,262,424 + +0.2 5,262,424 + +0.7 5,262,424 + -0.3 5,262,425 - -0.4 5,262,425 - -0.1 5,262,425 - -1.0 5,262,425 - +0.3 5,262,439 - -0.0 5,262,514 - -0.5 5,262,556 + -1.1 5,262,738 + +0.3 5,262,738 + +0.2 5,262,739 - +0.0 5,262,739 - +0.6 5,262,739 - -0.8 5,262,760 + -1.1 5,262,892 + Pf6N2E2_4529 0.14 -0.1 5,262,893 - Pf6N2E2_4529 0.14 +0.4 5,262,893 - Pf6N2E2_4529 0.14 -0.9 5,263,197 + +0.9 5,263,198 - Pf6N2E2_4529 0.41 -1.3 5,263,198 - Pf6N2E2_4529 0.41 +0.5 5,263,198 - Pf6N2E2_4529 0.41 -0.6 5,263,304 + Pf6N2E2_4529 0.51 -0.2 5,263,304 + Pf6N2E2_4529 0.51 +0.5 5,263,304 + Pf6N2E2_4529 0.51 +0.6 5,263,305 - Pf6N2E2_4529 0.51 +0.3 5,263,307 + Pf6N2E2_4529 0.51 +0.4 5,263,307 + Pf6N2E2_4529 0.51 -0.4 5,263,308 - Pf6N2E2_4529 0.51 -0.3 5,263,308 - Pf6N2E2_4529 0.51 +0.7 5,263,308 - Pf6N2E2_4529 0.51 +0.3
Or see this region's nucleotide sequence