Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_388 and Pf6N2E2_389 are separated by 216 nucleotides Pf6N2E2_389 and Pf6N2E2_390 are separated by 58 nucleotides Pf6N2E2_390 and Pf6N2E2_391 are separated by 48 nucleotides
Pf6N2E2_388 - Transporter, MFS superfamily, at 455,437 to 456,780
_388
Pf6N2E2_389 - Choline dehydrogenase (EC 1.1.99.1), at 456,997 to 458,562
_389
Pf6N2E2_390 - hypothetical protein, at 458,621 to 459,394
_390
Pf6N2E2_391 - hypothetical protein, at 459,443 to 460,921
_391
Position (kb) 456
457
458
459 Strain fitness (log2 ratio) -1
0
1
2
3 at 456.008 kb on + strand, within Pf6N2E2_388 at 456.008 kb on + strand, within Pf6N2E2_388 at 456.009 kb on - strand, within Pf6N2E2_388 at 456.028 kb on + strand, within Pf6N2E2_388 at 456.031 kb on - strand, within Pf6N2E2_388 at 456.149 kb on + strand, within Pf6N2E2_388 at 456.167 kb on + strand, within Pf6N2E2_388 at 456.167 kb on + strand, within Pf6N2E2_388 at 456.202 kb on - strand, within Pf6N2E2_388 at 456.209 kb on + strand, within Pf6N2E2_388 at 456.209 kb on + strand, within Pf6N2E2_388 at 456.254 kb on - strand, within Pf6N2E2_388 at 456.280 kb on + strand, within Pf6N2E2_388 at 456.280 kb on + strand, within Pf6N2E2_388 at 456.280 kb on + strand, within Pf6N2E2_388 at 456.530 kb on + strand, within Pf6N2E2_388 at 456.531 kb on - strand, within Pf6N2E2_388 at 456.531 kb on - strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.532 kb on + strand, within Pf6N2E2_388 at 456.533 kb on - strand, within Pf6N2E2_388 at 456.533 kb on - strand, within Pf6N2E2_388 at 456.533 kb on - strand, within Pf6N2E2_388 at 456.655 kb on + strand at 456.656 kb on - strand at 456.697 kb on + strand at 456.698 kb on - strand at 456.736 kb on + strand at 456.736 kb on + strand at 456.765 kb on + strand at 456.766 kb on - strand at 456.778 kb on + strand at 456.778 kb on + strand at 456.778 kb on + strand at 456.779 kb on - strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.875 kb on + strand at 456.881 kb on + strand at 456.881 kb on + strand at 456.938 kb on - strand at 456.938 kb on - strand at 456.966 kb on - strand at 456.966 kb on - strand at 456.998 kb on + strand at 457.143 kb on - strand at 457.143 kb on - strand at 457.214 kb on + strand, within Pf6N2E2_389 at 457.583 kb on + strand, within Pf6N2E2_389 at 457.583 kb on + strand, within Pf6N2E2_389 at 457.583 kb on + strand, within Pf6N2E2_389 at 457.584 kb on - strand, within Pf6N2E2_389 at 457.584 kb on - strand, within Pf6N2E2_389 at 457.632 kb on - strand, within Pf6N2E2_389 at 457.641 kb on + strand, within Pf6N2E2_389 at 457.808 kb on + strand, within Pf6N2E2_389 at 457.937 kb on + strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.938 kb on - strand, within Pf6N2E2_389 at 457.947 kb on - strand, within Pf6N2E2_389 at 457.978 kb on + strand, within Pf6N2E2_389 at 457.979 kb on - strand, within Pf6N2E2_389 at 457.979 kb on - strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.183 kb on + strand, within Pf6N2E2_389 at 458.538 kb on - strand at 458.538 kb on - strand at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.794 kb on - strand, within Pf6N2E2_390 at 458.943 kb on + strand, within Pf6N2E2_390 at 458.944 kb on - strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.054 kb on + strand, within Pf6N2E2_390 at 459.055 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.288 kb on - strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.310 kb on + strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.311 kb on - strand, within Pf6N2E2_390 at 459.459 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 456,008 + Pf6N2E2_388 0.42 +1.6 456,008 + Pf6N2E2_388 0.42 -0.1 456,009 - Pf6N2E2_388 0.43 -0.7 456,028 + Pf6N2E2_388 0.44 -0.0 456,031 - Pf6N2E2_388 0.44 -0.0 456,149 + Pf6N2E2_388 0.53 +0.2 456,167 + Pf6N2E2_388 0.54 +0.7 456,167 + Pf6N2E2_388 0.54 +0.7 456,202 - Pf6N2E2_388 0.57 +0.4 456,209 + Pf6N2E2_388 0.57 +0.1 456,209 + Pf6N2E2_388 0.57 -0.1 456,254 - Pf6N2E2_388 0.61 +0.7 456,280 + Pf6N2E2_388 0.63 +0.3 456,280 + Pf6N2E2_388 0.63 -0.5 456,280 + Pf6N2E2_388 0.63 +0.7 456,530 + Pf6N2E2_388 0.81 +0.3 456,531 - Pf6N2E2_388 0.81 -1.4 456,531 - Pf6N2E2_388 0.81 +0.6 456,532 + Pf6N2E2_388 0.81 -0.3 456,532 + Pf6N2E2_388 0.81 -0.9 456,532 + Pf6N2E2_388 0.81 -0.1 456,532 + Pf6N2E2_388 0.81 -0.7 456,533 - Pf6N2E2_388 0.82 -0.2 456,533 - Pf6N2E2_388 0.82 +0.2 456,533 - Pf6N2E2_388 0.82 -0.3 456,655 + -0.2 456,656 - +0.2 456,697 + -0.3 456,698 - +1.4 456,736 + +0.8 456,736 + -0.5 456,765 + -0.3 456,766 - -0.2 456,778 + +0.7 456,778 + -0.1 456,778 + +0.5 456,779 - -0.3 456,875 + -0.3 456,875 + +3.7 456,875 + -0.2 456,875 + +0.2 456,875 + +0.5 456,881 + -0.0 456,881 + +0.5 456,938 - +3.3 456,938 - -0.4 456,966 - -0.6 456,966 - -0.6 456,998 + +0.2 457,143 - -0.2 457,143 - +0.3 457,214 + Pf6N2E2_389 0.14 -0.9 457,583 + Pf6N2E2_389 0.37 +1.1 457,583 + Pf6N2E2_389 0.37 +0.7 457,583 + Pf6N2E2_389 0.37 -0.4 457,584 - Pf6N2E2_389 0.37 +1.0 457,584 - Pf6N2E2_389 0.37 -0.2 457,632 - Pf6N2E2_389 0.41 +2.0 457,641 + Pf6N2E2_389 0.41 -0.6 457,808 + Pf6N2E2_389 0.52 +0.0 457,937 + Pf6N2E2_389 0.60 +0.5 457,938 - Pf6N2E2_389 0.60 +0.2 457,938 - Pf6N2E2_389 0.60 +0.3 457,938 - Pf6N2E2_389 0.60 +0.4 457,938 - Pf6N2E2_389 0.60 +0.0 457,947 - Pf6N2E2_389 0.61 -0.4 457,978 + Pf6N2E2_389 0.63 -0.3 457,979 - Pf6N2E2_389 0.63 -0.2 457,979 - Pf6N2E2_389 0.63 -0.2 458,183 + Pf6N2E2_389 0.76 +0.1 458,183 + Pf6N2E2_389 0.76 +0.5 458,183 + Pf6N2E2_389 0.76 -0.2 458,538 - +1.1 458,538 - -1.1 458,794 - Pf6N2E2_390 0.22 +0.6 458,794 - Pf6N2E2_390 0.22 -0.9 458,794 - Pf6N2E2_390 0.22 +1.6 458,943 + Pf6N2E2_390 0.42 -0.2 458,944 - Pf6N2E2_390 0.42 +0.6 459,054 + Pf6N2E2_390 0.56 -0.9 459,054 + Pf6N2E2_390 0.56 +1.3 459,054 + Pf6N2E2_390 0.56 -0.4 459,055 - Pf6N2E2_390 0.56 +0.5 459,288 - Pf6N2E2_390 0.86 +0.2 459,288 - Pf6N2E2_390 0.86 +0.2 459,310 + Pf6N2E2_390 0.89 +0.4 459,310 + Pf6N2E2_390 0.89 +0.1 459,310 + Pf6N2E2_390 0.89 -0.7 459,311 - Pf6N2E2_390 0.89 +1.0 459,311 - Pf6N2E2_390 0.89 -0.3 459,311 - Pf6N2E2_390 0.89 +0.1 459,311 - Pf6N2E2_390 0.89 -0.0 459,311 - Pf6N2E2_390 0.89 +1.3 459,459 + +0.2
Or see this region's nucleotide sequence