Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3617

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3616 and Pf6N2E2_3617 overlap by 4 nucleotidesPf6N2E2_3617 and Pf6N2E2_3618 are separated by 3 nucleotidesPf6N2E2_3618 and Pf6N2E2_3619 overlap by 13 nucleotides Pf6N2E2_3616 - DNA mismatch repair protein MutL, at 4,210,481 to 4,212,394 _3616 Pf6N2E2_3617 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 4,212,391 to 4,213,818 _3617 Pf6N2E2_3618 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 4,213,822 to 4,214,289 _3618 Pf6N2E2_3619 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 4,214,277 to 4,215,776 _3619 Position (kb) 4212 4213 4214Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4211.404 kb on - strand, within Pf6N2E2_3616at 4211.442 kb on - strand, within Pf6N2E2_3616at 4211.442 kb on - strand, within Pf6N2E2_3616at 4211.442 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.601 kb on + strand, within Pf6N2E2_3616at 4211.602 kb on - strand, within Pf6N2E2_3616at 4211.602 kb on - strand, within Pf6N2E2_3616at 4211.608 kb on - strand, within Pf6N2E2_3616at 4212.038 kb on - strand, within Pf6N2E2_3616at 4212.596 kb on + strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4213.804 kb on - strandat 4213.824 kb on - strandat 4214.271 kb on - strandat 4214.578 kb on + strand, within Pf6N2E2_3619at 4214.579 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619at 4214.743 kb on - strand, within Pf6N2E2_3619

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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4,211,404 - Pf6N2E2_3616 0.48 -0.7
4,211,442 - Pf6N2E2_3616 0.50 -0.8
4,211,442 - Pf6N2E2_3616 0.50 +0.4
4,211,442 - Pf6N2E2_3616 0.50 +0.2
4,211,463 - Pf6N2E2_3616 0.51 +0.1
4,211,463 - Pf6N2E2_3616 0.51 +1.1
4,211,463 - Pf6N2E2_3616 0.51 -0.4
4,211,463 - Pf6N2E2_3616 0.51 -0.4
4,211,463 - Pf6N2E2_3616 0.51 -0.5
4,211,601 + Pf6N2E2_3616 0.59 +0.9
4,211,602 - Pf6N2E2_3616 0.59 +0.4
4,211,602 - Pf6N2E2_3616 0.59 +1.8
4,211,608 - Pf6N2E2_3616 0.59 +0.3
4,212,038 - Pf6N2E2_3616 0.81 -0.1
4,212,596 + Pf6N2E2_3617 0.14 -2.2
4,212,597 - Pf6N2E2_3617 0.14 -1.0
4,212,597 - Pf6N2E2_3617 0.14 -3.0
4,212,597 - Pf6N2E2_3617 0.14 -1.1
4,212,597 - Pf6N2E2_3617 0.14 -0.9
4,212,597 - Pf6N2E2_3617 0.14 -1.3
4,212,597 - Pf6N2E2_3617 0.14 -2.1
4,212,597 - Pf6N2E2_3617 0.14 -0.2
4,212,597 - Pf6N2E2_3617 0.14 -3.0
4,212,597 - Pf6N2E2_3617 0.14 -1.9
4,212,597 - Pf6N2E2_3617 0.14 -3.5
4,212,597 - Pf6N2E2_3617 0.14 -2.9
4,213,804 - -2.8
4,213,824 - -1.6
4,214,271 - -1.4
4,214,578 + Pf6N2E2_3619 0.20 +0.9
4,214,579 - Pf6N2E2_3619 0.20 +0.7
4,214,743 - Pf6N2E2_3619 0.31 +0.1
4,214,743 - Pf6N2E2_3619 0.31 +0.8
4,214,743 - Pf6N2E2_3619 0.31 +0.2
4,214,743 - Pf6N2E2_3619 0.31 -0.2
4,214,743 - Pf6N2E2_3619 0.31 +1.2

Or see this region's nucleotide sequence