Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_359

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_357 and Pf6N2E2_358 are separated by 21 nucleotidesPf6N2E2_358 and Pf6N2E2_359 are separated by 36 nucleotidesPf6N2E2_359 and Pf6N2E2_360 are separated by 86 nucleotides Pf6N2E2_357 - hypothetical protein, at 422,979 to 423,353 _357 Pf6N2E2_358 - Haloacid dehalogenase, type II, at 423,375 to 424,043 _358 Pf6N2E2_359 - Opine oxidase subunit B, at 424,080 to 425,354 _359 Pf6N2E2_360 - ABC peptide transporter, periplasmic ligand binding protein, at 425,441 to 427,084 _360 Position (kb) 424 425 426Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 423.137 kb on + strand, within Pf6N2E2_357at 423.137 kb on + strand, within Pf6N2E2_357at 423.138 kb on - strand, within Pf6N2E2_357at 423.138 kb on - strand, within Pf6N2E2_357at 423.282 kb on + strand, within Pf6N2E2_357at 423.282 kb on + strand, within Pf6N2E2_357at 423.283 kb on - strand, within Pf6N2E2_357at 423.283 kb on - strand, within Pf6N2E2_357at 423.396 kb on + strandat 423.396 kb on + strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.397 kb on - strandat 423.556 kb on - strand, within Pf6N2E2_358at 423.633 kb on + strand, within Pf6N2E2_358at 423.633 kb on + strand, within Pf6N2E2_358at 423.781 kb on - strand, within Pf6N2E2_358at 423.813 kb on + strand, within Pf6N2E2_358at 424.095 kb on + strandat 424.360 kb on - strand, within Pf6N2E2_359at 424.363 kb on - strand, within Pf6N2E2_359at 424.371 kb on + strand, within Pf6N2E2_359at 424.477 kb on - strandat 424.861 kb on + strand, within Pf6N2E2_359at 424.862 kb on - strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.908 kb on + strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.909 kb on - strand, within Pf6N2E2_359at 424.935 kb on + strand, within Pf6N2E2_359at 424.936 kb on - strand, within Pf6N2E2_359at 425.085 kb on + strand, within Pf6N2E2_359at 425.085 kb on + strand, within Pf6N2E2_359at 425.325 kb on + strandat 425.328 kb on + strandat 425.344 kb on - strandat 425.414 kb on + strandat 425.432 kb on - strandat 425.735 kb on + strand, within Pf6N2E2_360at 425.736 kb on - strand, within Pf6N2E2_360at 425.736 kb on - strand, within Pf6N2E2_360at 425.747 kb on + strand, within Pf6N2E2_360at 425.747 kb on + strand, within Pf6N2E2_360at 425.747 kb on + strand, within Pf6N2E2_360at 425.747 kb on + strand, within Pf6N2E2_360at 425.748 kb on - strand, within Pf6N2E2_360at 425.748 kb on - strand, within Pf6N2E2_360at 425.756 kb on + strand, within Pf6N2E2_360at 425.757 kb on - strand, within Pf6N2E2_360at 426.013 kb on - strand, within Pf6N2E2_360at 426.013 kb on - strand, within Pf6N2E2_360at 426.013 kb on - strand, within Pf6N2E2_360at 426.172 kb on + strand, within Pf6N2E2_360at 426.180 kb on - strand, within Pf6N2E2_360at 426.180 kb on - strand, within Pf6N2E2_360at 426.180 kb on - strand, within Pf6N2E2_360at 426.201 kb on - strand, within Pf6N2E2_360

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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423,137 + Pf6N2E2_357 0.42 +0.1
423,137 + Pf6N2E2_357 0.42 +0.4
423,138 - Pf6N2E2_357 0.42 -1.2
423,138 - Pf6N2E2_357 0.42 -0.2
423,282 + Pf6N2E2_357 0.81 -0.3
423,282 + Pf6N2E2_357 0.81 +1.1
423,283 - Pf6N2E2_357 0.81 -1.4
423,283 - Pf6N2E2_357 0.81 +0.1
423,396 + -0.0
423,396 + +0.7
423,397 - -0.2
423,397 - +0.8
423,397 - +0.1
423,397 - +0.5
423,397 - -0.1
423,397 - +1.4
423,556 - Pf6N2E2_358 0.27 +0.3
423,633 + Pf6N2E2_358 0.39 +0.0
423,633 + Pf6N2E2_358 0.39 +0.2
423,781 - Pf6N2E2_358 0.61 -0.5
423,813 + Pf6N2E2_358 0.65 -2.4
424,095 + +0.3
424,360 - Pf6N2E2_359 0.22 -0.1
424,363 - Pf6N2E2_359 0.22 -0.0
424,371 + Pf6N2E2_359 0.23 +0.1
424,477 - +2.8
424,861 + Pf6N2E2_359 0.61 +0.2
424,862 - Pf6N2E2_359 0.61 -0.2
424,908 + Pf6N2E2_359 0.65 -0.5
424,908 + Pf6N2E2_359 0.65 -0.4
424,908 + Pf6N2E2_359 0.65 -0.8
424,908 + Pf6N2E2_359 0.65 -0.3
424,908 + Pf6N2E2_359 0.65 -1.8
424,908 + Pf6N2E2_359 0.65 -0.2
424,908 + Pf6N2E2_359 0.65 -1.0
424,908 + Pf6N2E2_359 0.65 +0.3
424,908 + Pf6N2E2_359 0.65 -0.1
424,909 - Pf6N2E2_359 0.65 -0.3
424,909 - Pf6N2E2_359 0.65 +2.0
424,909 - Pf6N2E2_359 0.65 -0.2
424,909 - Pf6N2E2_359 0.65 +0.8
424,909 - Pf6N2E2_359 0.65 -0.1
424,909 - Pf6N2E2_359 0.65 -0.2
424,909 - Pf6N2E2_359 0.65 +0.2
424,935 + Pf6N2E2_359 0.67 -0.0
424,936 - Pf6N2E2_359 0.67 -0.0
425,085 + Pf6N2E2_359 0.79 -1.1
425,085 + Pf6N2E2_359 0.79 -0.8
425,325 + -0.1
425,328 + -2.9
425,344 - +1.2
425,414 + -1.0
425,432 - -0.6
425,735 + Pf6N2E2_360 0.18 +0.8
425,736 - Pf6N2E2_360 0.18 -0.1
425,736 - Pf6N2E2_360 0.18 -0.0
425,747 + Pf6N2E2_360 0.19 +0.1
425,747 + Pf6N2E2_360 0.19 -0.2
425,747 + Pf6N2E2_360 0.19 +0.3
425,747 + Pf6N2E2_360 0.19 -0.8
425,748 - Pf6N2E2_360 0.19 +1.9
425,748 - Pf6N2E2_360 0.19 -0.8
425,756 + Pf6N2E2_360 0.19 +0.1
425,757 - Pf6N2E2_360 0.19 +0.8
426,013 - Pf6N2E2_360 0.35 +0.0
426,013 - Pf6N2E2_360 0.35 +0.6
426,013 - Pf6N2E2_360 0.35 -0.2
426,172 + Pf6N2E2_360 0.44 -0.1
426,180 - Pf6N2E2_360 0.45 -1.0
426,180 - Pf6N2E2_360 0.45 +1.5
426,180 - Pf6N2E2_360 0.45 -0.5
426,201 - Pf6N2E2_360 0.46 -1.2

Or see this region's nucleotide sequence