Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_350

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_348 and Pf6N2E2_349 overlap by 4 nucleotidesPf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotidesPf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides Pf6N2E2_348 - Transcriptional regulator, LysR family, at 414,114 to 415,043 _348 Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774 _349 Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232 _350 Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686 _351 Position (kb) 415 416 417Strain fitness (log2 ratio) -2 -1 0 1 2at 414.849 kb on + strand, within Pf6N2E2_348at 414.849 kb on + strand, within Pf6N2E2_348at 414.849 kb on + strand, within Pf6N2E2_348at 414.849 kb on + strand, within Pf6N2E2_348at 414.849 kb on + strand, within Pf6N2E2_348at 414.850 kb on - strand, within Pf6N2E2_348at 414.850 kb on - strand, within Pf6N2E2_348at 414.850 kb on - strand, within Pf6N2E2_348at 414.850 kb on - strand, within Pf6N2E2_348at 414.927 kb on + strand, within Pf6N2E2_348at 414.927 kb on + strand, within Pf6N2E2_348at 415.062 kb on + strandat 415.062 kb on + strandat 415.063 kb on - strandat 415.063 kb on - strandat 415.073 kb on + strandat 415.073 kb on + strandat 415.074 kb on - strandat 415.074 kb on - strandat 415.074 kb on - strandat 415.082 kb on + strandat 415.083 kb on - strandat 415.083 kb on - strandat 415.347 kb on + strand, within Pf6N2E2_349at 415.515 kb on + strand, within Pf6N2E2_349at 415.516 kb on - strand, within Pf6N2E2_349at 415.542 kb on - strand, within Pf6N2E2_349at 415.660 kb on + strand, within Pf6N2E2_349at 415.660 kb on + strand, within Pf6N2E2_349at 415.772 kb on + strandat 415.773 kb on - strandat 415.806 kb on + strandat 415.807 kb on - strandat 415.807 kb on - strandat 415.807 kb on - strandat 415.807 kb on - strandat 415.865 kb on + strandat 416.131 kb on + strand, within Pf6N2E2_350at 416.192 kb on - strand, within Pf6N2E2_350at 416.231 kb on - strandat 416.231 kb on - strandat 416.272 kb on + strandat 416.272 kb on + strandat 416.272 kb on + strandat 416.297 kb on - strandat 416.323 kb on + strandat 416.449 kb on + strand, within Pf6N2E2_351at 416.584 kb on + strand, within Pf6N2E2_351at 416.584 kb on + strand, within Pf6N2E2_351at 416.585 kb on - strand, within Pf6N2E2_351at 417.212 kb on - strand, within Pf6N2E2_351

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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414,849 + Pf6N2E2_348 0.79 +1.0
414,849 + Pf6N2E2_348 0.79 +2.0
414,849 + Pf6N2E2_348 0.79 -0.2
414,849 + Pf6N2E2_348 0.79 +0.9
414,849 + Pf6N2E2_348 0.79 +0.3
414,850 - Pf6N2E2_348 0.79 +1.0
414,850 - Pf6N2E2_348 0.79 -0.3
414,850 - Pf6N2E2_348 0.79 -1.6
414,850 - Pf6N2E2_348 0.79 +0.6
414,927 + Pf6N2E2_348 0.87 +0.9
414,927 + Pf6N2E2_348 0.87 -0.2
415,062 + -0.8
415,062 + +1.8
415,063 - -1.8
415,063 - -0.0
415,073 + -2.7
415,073 + -0.5
415,074 - +0.5
415,074 - +1.8
415,074 - +0.3
415,082 + -0.2
415,083 - +0.2
415,083 - +0.8
415,347 + Pf6N2E2_349 0.42 +0.3
415,515 + Pf6N2E2_349 0.65 -0.0
415,516 - Pf6N2E2_349 0.65 +0.8
415,542 - Pf6N2E2_349 0.68 -0.4
415,660 + Pf6N2E2_349 0.84 +0.2
415,660 + Pf6N2E2_349 0.84 -0.8
415,772 + +0.4
415,773 - -0.4
415,806 + -0.2
415,807 - +0.2
415,807 - -0.8
415,807 - +0.5
415,807 - +0.7
415,865 + +0.4
416,131 + Pf6N2E2_350 0.74 -1.2
416,192 - Pf6N2E2_350 0.90 -0.7
416,231 - +0.5
416,231 - +0.8
416,272 + +0.8
416,272 + -0.2
416,272 + +0.5
416,297 - -1.1
416,323 + +1.1
416,449 + Pf6N2E2_351 0.15 +0.0
416,584 + Pf6N2E2_351 0.24 +1.1
416,584 + Pf6N2E2_351 0.24 -0.3
416,585 - Pf6N2E2_351 0.24 -1.2
417,212 - Pf6N2E2_351 0.67 -0.3

Or see this region's nucleotide sequence