Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3348 and Pf6N2E2_6117 are separated by 13 nucleotides Pf6N2E2_6117 and Pf6N2E2_3349 are separated by 125 nucleotides Pf6N2E2_3349 and Pf6N2E2_3350 are separated by 194 nucleotides Pf6N2E2_3350 and Pf6N2E2_3351 are separated by 143 nucleotides
Pf6N2E2_3348 - hypothetical protein, at 3,876,297 to 3,876,845
_3348
Pf6N2E2_6117 - tRNA-Thr-CGT, at 3,876,859 to 3,876,931
_6117
Pf6N2E2_3349 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 3,877,057 to 3,879,162
_3349
Pf6N2E2_3350 - SSU ribosomal protein S15p (S13e), at 3,879,357 to 3,879,626
_3350
Pf6N2E2_3351 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 3,879,770 to 3,880,687
_3351
Position (kb)
3877
3878
3879
3880 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3876.077 kb on + strand at 3876.078 kb on - strand at 3876.145 kb on + strand at 3876.145 kb on + strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.147 kb on + strand at 3876.147 kb on + strand at 3876.148 kb on - strand at 3876.148 kb on - strand at 3876.169 kb on - strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.209 kb on - strand at 3876.417 kb on + strand, within Pf6N2E2_3348 at 3876.417 kb on + strand, within Pf6N2E2_3348 at 3876.429 kb on - strand, within Pf6N2E2_3348 at 3876.445 kb on - strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.844 kb on - strand at 3876.844 kb on - strand at 3876.923 kb on + strand, within Pf6N2E2_6117 at 3876.924 kb on - strand at 3876.944 kb on + strand at 3876.944 kb on + strand at 3876.944 kb on + strand at 3876.945 kb on - strand at 3876.945 kb on - strand at 3876.996 kb on + strand at 3876.996 kb on + strand at 3876.997 kb on - strand at 3876.997 kb on - strand at 3876.997 kb on - strand at 3877.286 kb on + strand, within Pf6N2E2_3349 at 3877.286 kb on + strand, within Pf6N2E2_3349 at 3877.286 kb on + strand, within Pf6N2E2_3349 at 3877.287 kb on - strand, within Pf6N2E2_3349 at 3877.287 kb on - strand, within Pf6N2E2_3349 at 3879.272 kb on - strand at 3879.272 kb on - strand at 3879.848 kb on - strand at 3879.897 kb on - strand, within Pf6N2E2_3351 at 3879.919 kb on - strand, within Pf6N2E2_3351 at 3880.102 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 3,876,077 + +0.7 3,876,078 - +0.1 3,876,145 + +0.6 3,876,145 + +0.3 3,876,146 - -0.2 3,876,146 - -0.3 3,876,146 - -0.1 3,876,146 - +0.7 3,876,146 - +1.0 3,876,146 - -0.1 3,876,147 + -0.2 3,876,147 + -0.8 3,876,148 - +0.7 3,876,148 - -0.2 3,876,169 - -0.2 3,876,180 + -0.4 3,876,180 + -0.1 3,876,180 + +0.4 3,876,180 + +0.3 3,876,180 + +1.2 3,876,180 + -0.6 3,876,180 + -0.1 3,876,180 + -0.4 3,876,180 + -0.3 3,876,181 - -0.4 3,876,181 - +0.2 3,876,181 - +0.7 3,876,181 - +0.6 3,876,181 - +0.5 3,876,181 - -0.3 3,876,186 + +0.3 3,876,186 + +0.0 3,876,186 + -0.1 3,876,186 + +0.1 3,876,187 - +0.1 3,876,187 - -0.2 3,876,187 - -0.3 3,876,187 - -0.1 3,876,209 - +0.1 3,876,417 + Pf6N2E2_3348 0.22 +1.2 3,876,417 + Pf6N2E2_3348 0.22 +0.4 3,876,429 - Pf6N2E2_3348 0.24 +0.3 3,876,445 - Pf6N2E2_3348 0.27 +0.2 3,876,462 + Pf6N2E2_3348 0.30 +2.5 3,876,462 + Pf6N2E2_3348 0.30 +1.0 3,876,462 + Pf6N2E2_3348 0.30 -0.2 3,876,462 + Pf6N2E2_3348 0.30 -0.2 3,876,462 + Pf6N2E2_3348 0.30 +0.2 3,876,462 + Pf6N2E2_3348 0.30 +0.8 3,876,463 - Pf6N2E2_3348 0.30 +1.0 3,876,463 - Pf6N2E2_3348 0.30 -2.2 3,876,463 - Pf6N2E2_3348 0.30 +1.1 3,876,463 - Pf6N2E2_3348 0.30 -0.2 3,876,463 - Pf6N2E2_3348 0.30 +0.6 3,876,844 - -0.0 3,876,844 - -0.6 3,876,923 + Pf6N2E2_6117 0.88 +0.3 3,876,924 - +0.7 3,876,944 + +0.7 3,876,944 + +2.2 3,876,944 + +0.6 3,876,945 - +0.9 3,876,945 - +1.0 3,876,996 + +0.0 3,876,996 + +0.7 3,876,997 - +0.8 3,876,997 - +0.4 3,876,997 - -0.3 3,877,286 + Pf6N2E2_3349 0.11 -0.6 3,877,286 + Pf6N2E2_3349 0.11 -1.5 3,877,286 + Pf6N2E2_3349 0.11 -1.4 3,877,287 - Pf6N2E2_3349 0.11 +0.5 3,877,287 - Pf6N2E2_3349 0.11 -0.9 3,879,272 - +0.5 3,879,272 - -0.2 3,879,848 - -0.2 3,879,897 - Pf6N2E2_3351 0.14 -0.9 3,879,919 - Pf6N2E2_3351 0.16 -1.8 3,880,102 - Pf6N2E2_3351 0.36 -0.9 3,880,153 - Pf6N2E2_3351 0.42 +0.8 3,880,153 - Pf6N2E2_3351 0.42 -0.6 3,880,153 - Pf6N2E2_3351 0.42 +0.1 3,880,153 - Pf6N2E2_3351 0.42 -2.4
Or see this region's nucleotide sequence