Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_289 and Pf6N2E2_290 are separated by 77 nucleotides Pf6N2E2_290 and Pf6N2E2_291 are separated by 343 nucleotides Pf6N2E2_291 and Pf6N2E2_292 are separated by 29 nucleotides Pf6N2E2_292 and Pf6N2E2_293 are separated by 217 nucleotides Pf6N2E2_293 and Pf6N2E2_294 are separated by 123 nucleotides
Pf6N2E2_289 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 358,365 to 359,621
_289
Pf6N2E2_290 - Cold shock protein CspD, at 359,699 to 359,968
_290
Pf6N2E2_291 - ATP-dependent Clp protease adaptor protein ClpS, at 360,312 to 360,557
_291
Pf6N2E2_292 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 360,587 to 362,857
_292
Pf6N2E2_293 - Translation initiation factor 1, at 363,075 to 363,293
_293
Pf6N2E2_294 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 363,417 to 364,124
_294
Position (kb) 360
361
362
363 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 359.619 kb on + strand at 359.619 kb on + strand at 359.619 kb on + strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.620 kb on - strand at 359.836 kb on + strand, within Pf6N2E2_290 at 359.836 kb on + strand, within Pf6N2E2_290 at 359.837 kb on - strand, within Pf6N2E2_290 at 359.837 kb on - strand, within Pf6N2E2_290 at 359.838 kb on + strand, within Pf6N2E2_290 at 359.838 kb on + strand, within Pf6N2E2_290 at 359.838 kb on + strand, within Pf6N2E2_290 at 359.839 kb on - strand, within Pf6N2E2_290 at 359.862 kb on + strand, within Pf6N2E2_290 at 359.968 kb on + strand at 359.968 kb on + strand at 360.007 kb on - strand at 360.007 kb on - strand at 360.007 kb on - strand at 360.007 kb on - strand at 360.029 kb on + strand at 360.030 kb on - strand at 360.040 kb on + strand at 360.040 kb on + strand at 360.040 kb on + strand at 360.041 kb on - strand at 360.041 kb on - strand at 360.041 kb on - strand at 360.047 kb on - strand at 360.083 kb on - strand at 360.083 kb on - strand at 360.092 kb on - strand at 360.092 kb on - strand at 360.165 kb on + strand at 360.165 kb on + strand at 360.176 kb on + strand at 360.176 kb on + strand at 360.177 kb on - strand at 360.177 kb on - strand at 360.221 kb on - strand at 360.221 kb on - strand at 360.248 kb on - strand at 360.298 kb on + strand at 360.298 kb on + strand at 360.299 kb on - strand at 360.315 kb on + strand at 360.315 kb on + strand at 360.315 kb on + strand at 360.315 kb on + strand at 360.316 kb on - strand at 360.342 kb on + strand, within Pf6N2E2_291 at 360.342 kb on + strand, within Pf6N2E2_291 at 360.342 kb on + strand, within Pf6N2E2_291 at 360.431 kb on + strand, within Pf6N2E2_291 at 360.431 kb on + strand, within Pf6N2E2_291 at 360.432 kb on - strand, within Pf6N2E2_291 at 360.448 kb on + strand, within Pf6N2E2_291 at 360.448 kb on + strand, within Pf6N2E2_291 at 360.449 kb on - strand, within Pf6N2E2_291 at 360.530 kb on - strand, within Pf6N2E2_291 at 360.573 kb on + strand at 360.586 kb on + strand at 360.586 kb on + strand at 360.586 kb on + strand at 360.586 kb on + strand at 360.587 kb on - strand at 360.587 kb on - strand at 360.587 kb on - strand at 360.591 kb on + strand at 360.688 kb on - strand at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.870 kb on + strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.871 kb on - strand, within Pf6N2E2_292 at 360.995 kb on + strand, within Pf6N2E2_292 at 360.996 kb on - strand, within Pf6N2E2_292 at 361.058 kb on - strand, within Pf6N2E2_292 at 361.113 kb on - strand, within Pf6N2E2_292 at 361.334 kb on + strand, within Pf6N2E2_292 at 361.334 kb on + strand, within Pf6N2E2_292 at 361.334 kb on + strand, within Pf6N2E2_292 at 361.335 kb on - strand, within Pf6N2E2_292 at 361.666 kb on + strand, within Pf6N2E2_292 at 361.760 kb on + strand, within Pf6N2E2_292 at 361.761 kb on - strand, within Pf6N2E2_292 at 361.761 kb on - strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.168 kb on + strand, within Pf6N2E2_292 at 362.169 kb on - strand, within Pf6N2E2_292 at 362.169 kb on - strand, within Pf6N2E2_292 at 362.216 kb on + strand, within Pf6N2E2_292 at 362.216 kb on + strand, within Pf6N2E2_292 at 362.217 kb on - strand, within Pf6N2E2_292 at 362.217 kb on - strand, within Pf6N2E2_292 at 362.277 kb on + strand, within Pf6N2E2_292 at 362.278 kb on - strand, within Pf6N2E2_292 at 362.907 kb on - strand at 362.907 kb on - strand at 363.396 kb on - strand at 363.396 kb on - strand at 363.402 kb on - strand at 363.402 kb on - strand at 363.402 kb on - strand at 363.404 kb on - strand at 363.479 kb on - strand at 363.517 kb on + strand, within Pf6N2E2_294 at 363.518 kb on - strand, within Pf6N2E2_294 at 363.518 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.608 kb on - strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.616 kb on + strand, within Pf6N2E2_294 at 363.617 kb on - strand, within Pf6N2E2_294 at 363.692 kb on - strand, within Pf6N2E2_294 at 363.692 kb on - strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.754 kb on + strand, within Pf6N2E2_294 at 363.755 kb on - strand, within Pf6N2E2_294 at 363.755 kb on - strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.787 kb on + strand, within Pf6N2E2_294 at 363.788 kb on - strand, within Pf6N2E2_294 at 363.797 kb on - strand, within Pf6N2E2_294 at 363.808 kb on + strand, within Pf6N2E2_294 at 363.808 kb on + strand, within Pf6N2E2_294 at 363.809 kb on - strand, within Pf6N2E2_294 at 363.809 kb on - strand, within Pf6N2E2_294
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 359,619 + +0.8 359,619 + -1.1 359,619 + +0.9 359,620 - +1.1 359,620 - +0.5 359,620 - +0.0 359,620 - +0.1 359,620 - +0.5 359,620 - -0.3 359,836 + Pf6N2E2_290 0.51 +0.8 359,836 + Pf6N2E2_290 0.51 +0.4 359,837 - Pf6N2E2_290 0.51 -0.4 359,837 - Pf6N2E2_290 0.51 +1.3 359,838 + Pf6N2E2_290 0.51 -0.4 359,838 + Pf6N2E2_290 0.51 +2.8 359,838 + Pf6N2E2_290 0.51 +1.5 359,839 - Pf6N2E2_290 0.52 +0.0 359,862 + Pf6N2E2_290 0.60 +0.8 359,968 + +0.1 359,968 + -0.4 360,007 - -0.9 360,007 - +1.3 360,007 - +0.4 360,007 - +1.0 360,029 + -0.2 360,030 - +0.2 360,040 + +0.8 360,040 + -0.3 360,040 + +0.3 360,041 - +1.2 360,041 - -0.3 360,041 - -0.9 360,047 - +0.5 360,083 - +1.3 360,083 - +0.4 360,092 - +1.0 360,092 - +0.0 360,165 + +0.8 360,165 + -0.1 360,176 + +0.1 360,176 + +0.5 360,177 - -0.6 360,177 - +0.8 360,221 - -0.2 360,221 - -0.1 360,248 - -0.0 360,298 + +1.1 360,298 + +1.3 360,299 - -1.4 360,315 + +0.3 360,315 + +1.1 360,315 + -0.2 360,315 + -0.8 360,316 - -0.1 360,342 + Pf6N2E2_291 0.12 -0.1 360,342 + Pf6N2E2_291 0.12 -0.2 360,342 + Pf6N2E2_291 0.12 -1.7 360,431 + Pf6N2E2_291 0.48 -0.5 360,431 + Pf6N2E2_291 0.48 -0.3 360,432 - Pf6N2E2_291 0.49 +0.1 360,448 + Pf6N2E2_291 0.55 -1.5 360,448 + Pf6N2E2_291 0.55 -1.1 360,449 - Pf6N2E2_291 0.56 -0.5 360,530 - Pf6N2E2_291 0.89 -1.1 360,573 + -0.5 360,586 + -1.8 360,586 + -0.8 360,586 + -1.0 360,586 + +1.4 360,587 - -1.0 360,587 - -0.6 360,587 - +3.4 360,591 + -2.2 360,688 - -0.3 360,870 + Pf6N2E2_292 0.12 -0.9 360,870 + Pf6N2E2_292 0.12 -0.9 360,870 + Pf6N2E2_292 0.12 -0.2 360,870 + Pf6N2E2_292 0.12 +0.6 360,870 + Pf6N2E2_292 0.12 -1.1 360,871 - Pf6N2E2_292 0.13 -0.3 360,871 - Pf6N2E2_292 0.13 -1.0 360,871 - Pf6N2E2_292 0.13 -2.3 360,871 - Pf6N2E2_292 0.13 +0.1 360,995 + Pf6N2E2_292 0.18 -1.4 360,996 - Pf6N2E2_292 0.18 -0.8 361,058 - Pf6N2E2_292 0.21 -0.0 361,113 - Pf6N2E2_292 0.23 -2.5 361,334 + Pf6N2E2_292 0.33 +0.6 361,334 + Pf6N2E2_292 0.33 -0.8 361,334 + Pf6N2E2_292 0.33 -1.5 361,335 - Pf6N2E2_292 0.33 -0.6 361,666 + Pf6N2E2_292 0.48 -0.3 361,760 + Pf6N2E2_292 0.52 -0.3 361,761 - Pf6N2E2_292 0.52 -1.5 361,761 - Pf6N2E2_292 0.52 -0.6 362,168 + Pf6N2E2_292 0.70 +0.1 362,168 + Pf6N2E2_292 0.70 -1.0 362,168 + Pf6N2E2_292 0.70 -0.0 362,168 + Pf6N2E2_292 0.70 -1.2 362,169 - Pf6N2E2_292 0.70 -0.3 362,169 - Pf6N2E2_292 0.70 -2.1 362,216 + Pf6N2E2_292 0.72 -0.9 362,216 + Pf6N2E2_292 0.72 -0.4 362,217 - Pf6N2E2_292 0.72 -1.0 362,217 - Pf6N2E2_292 0.72 -2.2 362,277 + Pf6N2E2_292 0.74 +0.0 362,278 - Pf6N2E2_292 0.74 -1.8 362,907 - +0.5 362,907 - +0.9 363,396 - -1.0 363,396 - +0.0 363,402 - +0.5 363,402 - -0.8 363,402 - +0.8 363,404 - +0.2 363,479 - +0.8 363,517 + Pf6N2E2_294 0.14 -1.0 363,518 - Pf6N2E2_294 0.14 -0.2 363,518 - Pf6N2E2_294 0.14 +0.5 363,608 - Pf6N2E2_294 0.27 +0.8 363,608 - Pf6N2E2_294 0.27 -0.7 363,608 - Pf6N2E2_294 0.27 -0.6 363,616 + Pf6N2E2_294 0.28 -0.2 363,616 + Pf6N2E2_294 0.28 -0.1 363,616 + Pf6N2E2_294 0.28 -0.2 363,617 - Pf6N2E2_294 0.28 +1.5 363,692 - Pf6N2E2_294 0.39 -0.0 363,692 - Pf6N2E2_294 0.39 -1.3 363,754 + Pf6N2E2_294 0.48 +0.7 363,754 + Pf6N2E2_294 0.48 +0.1 363,754 + Pf6N2E2_294 0.48 +0.1 363,755 - Pf6N2E2_294 0.48 -1.2 363,755 - Pf6N2E2_294 0.48 +0.2 363,787 + Pf6N2E2_294 0.52 -0.0 363,787 + Pf6N2E2_294 0.52 -1.7 363,787 + Pf6N2E2_294 0.52 +0.2 363,787 + Pf6N2E2_294 0.52 +1.4 363,788 - Pf6N2E2_294 0.52 +1.8 363,797 - Pf6N2E2_294 0.54 +0.8 363,808 + Pf6N2E2_294 0.55 -0.5 363,808 + Pf6N2E2_294 0.55 -0.1 363,809 - Pf6N2E2_294 0.55 +0.2 363,809 - Pf6N2E2_294 0.55 -0.7
Or see this region's nucleotide sequence