Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2824 and Pf6N2E2_2825 overlap by 4 nucleotides Pf6N2E2_2825 and Pf6N2E2_2826 are separated by 442 nucleotides Pf6N2E2_2826 and Pf6N2E2_2827 are separated by 252 nucleotides
Pf6N2E2_2824 - RND efflux system, inner membrane transporter CmeB, at 3,310,688 to 3,313,852
_2824
Pf6N2E2_2825 - RND efflux system, outer membrane lipoprotein CmeC, at 3,313,849 to 3,315,306
_2825
Pf6N2E2_2826 - hypothetical protein, at 3,315,749 to 3,316,039
_2826
Pf6N2E2_2827 - hypothetical protein, at 3,316,292 to 3,316,642
_2827
Position (kb)
3313
3314
3315
3316 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3312.964 kb on + strand, within Pf6N2E2_2824 at 3312.964 kb on + strand, within Pf6N2E2_2824 at 3312.968 kb on + strand, within Pf6N2E2_2824 at 3312.968 kb on + strand, within Pf6N2E2_2824 at 3312.968 kb on + strand, within Pf6N2E2_2824 at 3312.968 kb on + strand, within Pf6N2E2_2824 at 3312.969 kb on - strand, within Pf6N2E2_2824 at 3312.969 kb on - strand, within Pf6N2E2_2824 at 3313.010 kb on + strand, within Pf6N2E2_2824 at 3313.010 kb on + strand, within Pf6N2E2_2824 at 3313.010 kb on + strand, within Pf6N2E2_2824 at 3313.011 kb on - strand, within Pf6N2E2_2824 at 3313.064 kb on + strand, within Pf6N2E2_2824 at 3313.065 kb on - strand, within Pf6N2E2_2824 at 3313.065 kb on - strand, within Pf6N2E2_2824 at 3313.287 kb on - strand, within Pf6N2E2_2824 at 3313.370 kb on + strand, within Pf6N2E2_2824 at 3313.472 kb on + strand, within Pf6N2E2_2824 at 3313.472 kb on + strand, within Pf6N2E2_2824 at 3313.472 kb on + strand, within Pf6N2E2_2824 at 3313.597 kb on + strand at 3313.598 kb on - strand at 3314.113 kb on + strand, within Pf6N2E2_2825 at 3314.114 kb on - strand, within Pf6N2E2_2825 at 3314.125 kb on + strand, within Pf6N2E2_2825 at 3314.266 kb on + strand, within Pf6N2E2_2825 at 3314.324 kb on - strand, within Pf6N2E2_2825 at 3314.389 kb on + strand, within Pf6N2E2_2825 at 3314.449 kb on + strand, within Pf6N2E2_2825 at 3314.965 kb on + strand, within Pf6N2E2_2825 at 3315.028 kb on + strand, within Pf6N2E2_2825 at 3315.028 kb on + strand, within Pf6N2E2_2825 at 3315.079 kb on + strand, within Pf6N2E2_2825 at 3315.080 kb on - strand, within Pf6N2E2_2825 at 3315.082 kb on + strand, within Pf6N2E2_2825 at 3315.082 kb on + strand, within Pf6N2E2_2825 at 3315.083 kb on - strand, within Pf6N2E2_2825 at 3315.220 kb on + strand at 3315.221 kb on - strand at 3315.221 kb on - strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.307 kb on + strand at 3315.308 kb on - strand at 3315.308 kb on - strand at 3315.308 kb on - strand at 3315.308 kb on - strand at 3315.308 kb on - strand at 3315.360 kb on + strand at 3315.361 kb on - strand at 3315.452 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.543 kb on - strand at 3315.545 kb on - strand at 3315.718 kb on + strand at 3315.719 kb on - strand at 3315.719 kb on - strand at 3315.719 kb on - strand at 3315.719 kb on - strand at 3315.723 kb on - strand at 3315.723 kb on - strand at 3316.157 kb on + strand at 3316.157 kb on + strand at 3316.157 kb on + strand at 3316.208 kb on + strand at 3316.278 kb on + strand at 3316.278 kb on + strand at 3316.278 kb on + strand at 3316.279 kb on - strand at 3316.279 kb on - strand at 3316.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 3,312,964 + Pf6N2E2_2824 0.72 -2.5 3,312,964 + Pf6N2E2_2824 0.72 -2.5 3,312,968 + Pf6N2E2_2824 0.72 -2.1 3,312,968 + Pf6N2E2_2824 0.72 -3.3 3,312,968 + Pf6N2E2_2824 0.72 -3.5 3,312,968 + Pf6N2E2_2824 0.72 -3.2 3,312,969 - Pf6N2E2_2824 0.72 -2.5 3,312,969 - Pf6N2E2_2824 0.72 -1.4 3,313,010 + Pf6N2E2_2824 0.73 -2.6 3,313,010 + Pf6N2E2_2824 0.73 -2.2 3,313,010 + Pf6N2E2_2824 0.73 -3.1 3,313,011 - Pf6N2E2_2824 0.73 -3.0 3,313,064 + Pf6N2E2_2824 0.75 -2.8 3,313,065 - Pf6N2E2_2824 0.75 -2.8 3,313,065 - Pf6N2E2_2824 0.75 -2.1 3,313,287 - Pf6N2E2_2824 0.82 -2.2 3,313,370 + Pf6N2E2_2824 0.85 -2.5 3,313,472 + Pf6N2E2_2824 0.88 -3.2 3,313,472 + Pf6N2E2_2824 0.88 -1.6 3,313,472 + Pf6N2E2_2824 0.88 -0.6 3,313,597 + -2.4 3,313,598 - +0.9 3,314,113 + Pf6N2E2_2825 0.18 -1.4 3,314,114 - Pf6N2E2_2825 0.18 -1.9 3,314,125 + Pf6N2E2_2825 0.19 +0.9 3,314,266 + Pf6N2E2_2825 0.29 -2.8 3,314,324 - Pf6N2E2_2825 0.33 -0.6 3,314,389 + Pf6N2E2_2825 0.37 -1.1 3,314,449 + Pf6N2E2_2825 0.41 -0.6 3,314,965 + Pf6N2E2_2825 0.77 -3.0 3,315,028 + Pf6N2E2_2825 0.81 -3.2 3,315,028 + Pf6N2E2_2825 0.81 -2.8 3,315,079 + Pf6N2E2_2825 0.84 -2.4 3,315,080 - Pf6N2E2_2825 0.84 -1.2 3,315,082 + Pf6N2E2_2825 0.85 -1.4 3,315,082 + Pf6N2E2_2825 0.85 -3.5 3,315,083 - Pf6N2E2_2825 0.85 -1.1 3,315,220 + -2.9 3,315,221 - -0.1 3,315,221 - -0.6 3,315,307 + -1.2 3,315,307 + -1.1 3,315,307 + -1.4 3,315,307 + -1.7 3,315,307 + -0.9 3,315,307 + -1.5 3,315,307 + -1.1 3,315,307 + -1.2 3,315,308 - -0.4 3,315,308 - -2.2 3,315,308 - -1.9 3,315,308 - -3.2 3,315,308 - -2.9 3,315,360 + +0.2 3,315,361 - +1.2 3,315,452 - -0.2 3,315,543 - -0.2 3,315,543 - -0.7 3,315,543 - +0.4 3,315,543 - +0.7 3,315,543 - +0.2 3,315,543 - +0.3 3,315,543 - +0.3 3,315,543 - +0.4 3,315,545 - -0.3 3,315,718 + +0.2 3,315,719 - +0.2 3,315,719 - +0.3 3,315,719 - -0.8 3,315,719 - +0.2 3,315,723 - +0.0 3,315,723 - +0.3 3,316,157 + -0.6 3,316,157 + +0.2 3,316,157 + +0.4 3,316,208 + +0.6 3,316,278 + +0.2 3,316,278 + -0.2 3,316,278 + -0.3 3,316,279 - -0.8 3,316,279 - +1.4 3,316,279 - -1.0
Or see this region's nucleotide sequence