Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1 and Pf6N2E2_2 are separated by 145 nucleotides Pf6N2E2_2 and Pf6N2E2_3 are separated by 273 nucleotides
Pf6N2E2_1 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 90 to 860
_1
Pf6N2E2_2 - FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis, at 1,006 to 1,740
_2
Pf6N2E2_3 - Non-ribosomal peptide synthetase modules, pyoverdine, at 2,014 to 14,454
_3
Position (kb) 1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 0.027 kb on + strand at 0.028 kb on - strand at 0.035 kb on - strand at 0.035 kb on - strand at 0.158 kb on - strand at 0.181 kb on + strand, within Pf6N2E2_1 at 0.182 kb on - strand, within Pf6N2E2_1 at 0.193 kb on + strand, within Pf6N2E2_1 at 0.298 kb on + strand, within Pf6N2E2_1 at 0.349 kb on + strand, within Pf6N2E2_1 at 0.353 kb on - strand, within Pf6N2E2_1 at 0.353 kb on - strand, within Pf6N2E2_1 at 0.369 kb on + strand, within Pf6N2E2_1 at 0.370 kb on - strand, within Pf6N2E2_1 at 0.370 kb on - strand, within Pf6N2E2_1 at 0.838 kb on + strand at 0.839 kb on - strand at 0.839 kb on - strand at 0.839 kb on - strand at 0.902 kb on + strand at 0.902 kb on + strand at 0.902 kb on + strand at 0.903 kb on - strand at 0.904 kb on + strand at 0.904 kb on + strand at 0.904 kb on + strand at 0.904 kb on + strand at 0.905 kb on - strand at 0.905 kb on - strand at 0.948 kb on - strand at 0.955 kb on + strand at 0.955 kb on + strand at 1.037 kb on - strand at 1.060 kb on + strand at 1.060 kb on + strand at 1.060 kb on + strand at 1.061 kb on - strand at 1.349 kb on - strand, within Pf6N2E2_2 at 1.430 kb on + strand, within Pf6N2E2_2 at 1.431 kb on - strand, within Pf6N2E2_2 at 1.431 kb on - strand, within Pf6N2E2_2 at 1.431 kb on - strand, within Pf6N2E2_2 at 1.960 kb on - strand at 2.173 kb on + strand at 2.227 kb on + strand at 2.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 27 + +1.2 28 - +0.4 35 - +0.2 35 - -0.2 158 - -0.2 181 + Pf6N2E2_1 0.12 -0.5 182 - Pf6N2E2_1 0.12 -0.0 193 + Pf6N2E2_1 0.13 -0.2 298 + Pf6N2E2_1 0.27 -0.3 349 + Pf6N2E2_1 0.34 +0.7 353 - Pf6N2E2_1 0.34 +1.1 353 - Pf6N2E2_1 0.34 -0.3 369 + Pf6N2E2_1 0.36 -0.1 370 - Pf6N2E2_1 0.36 +0.5 370 - Pf6N2E2_1 0.36 +1.2 838 + +0.4 839 - -0.5 839 - -2.9 839 - +0.2 902 + +0.4 902 + -0.2 902 + -0.3 903 - -0.2 904 + +0.2 904 + -0.9 904 + +0.7 904 + +0.4 905 - +1.2 905 - +1.8 948 - +0.4 955 + -1.3 955 + +1.3 1,037 - +0.1 1,060 + +0.4 1,060 + -0.2 1,060 + +0.8 1,061 - -1.2 1,349 - Pf6N2E2_2 0.47 -0.9 1,430 + Pf6N2E2_2 0.58 -0.0 1,431 - Pf6N2E2_2 0.58 -1.0 1,431 - Pf6N2E2_2 0.58 +1.0 1,431 - Pf6N2E2_2 0.58 -1.4 1,960 - +0.4 2,173 + +0.2 2,227 + +0.1 2,228 - +1.6
Or see this region's nucleotide sequence