Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1904 and Pf6N2E2_1905 are separated by 129 nucleotides Pf6N2E2_1905 and Pf6N2E2_1906 are separated by 272 nucleotides
Pf6N2E2_1904 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 2,255,744 to 2,258,002
_1904
Pf6N2E2_1905 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 2,258,132 to 2,258,578
_1905
Pf6N2E2_1906 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,258,851 to 2,260,302
_1906
Position (kb)
2258
2259 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2257.299 kb on + strand, within Pf6N2E2_1904 at 2257.299 kb on + strand, within Pf6N2E2_1904 at 2257.309 kb on + strand, within Pf6N2E2_1904 at 2257.326 kb on + strand, within Pf6N2E2_1904 at 2257.326 kb on + strand, within Pf6N2E2_1904 at 2257.443 kb on + strand, within Pf6N2E2_1904 at 2257.444 kb on - strand, within Pf6N2E2_1904 at 2257.444 kb on - strand, within Pf6N2E2_1904 at 2257.541 kb on + strand, within Pf6N2E2_1904 at 2257.542 kb on - strand, within Pf6N2E2_1904 at 2257.584 kb on + strand, within Pf6N2E2_1904 at 2257.584 kb on + strand, within Pf6N2E2_1904 at 2257.584 kb on + strand, within Pf6N2E2_1904 at 2257.585 kb on - strand, within Pf6N2E2_1904 at 2257.585 kb on - strand, within Pf6N2E2_1904 at 2257.585 kb on - strand, within Pf6N2E2_1904 at 2257.691 kb on + strand, within Pf6N2E2_1904 at 2257.692 kb on - strand, within Pf6N2E2_1904 at 2257.692 kb on - strand, within Pf6N2E2_1904 at 2257.692 kb on - strand, within Pf6N2E2_1904 at 2257.754 kb on - strand, within Pf6N2E2_1904 at 2258.002 kb on + strand at 2258.002 kb on + strand at 2258.002 kb on + strand at 2258.003 kb on - strand at 2258.003 kb on - strand at 2258.064 kb on - strand at 2258.174 kb on - strand at 2258.174 kb on - strand at 2258.175 kb on + strand at 2258.175 kb on + strand at 2258.176 kb on - strand at 2258.176 kb on - strand at 2258.475 kb on + strand, within Pf6N2E2_1905 at 2258.475 kb on + strand, within Pf6N2E2_1905 at 2258.476 kb on - strand, within Pf6N2E2_1905 at 2258.476 kb on - strand, within Pf6N2E2_1905 at 2258.476 kb on - strand, within Pf6N2E2_1905 at 2258.516 kb on + strand, within Pf6N2E2_1905 at 2258.516 kb on + strand, within Pf6N2E2_1905 at 2258.528 kb on - strand, within Pf6N2E2_1905 at 2258.550 kb on + strand at 2258.550 kb on + strand at 2258.551 kb on - strand at 2258.551 kb on - strand at 2258.551 kb on - strand at 2258.564 kb on + strand at 2258.581 kb on + strand at 2258.582 kb on - strand at 2258.582 kb on - strand at 2258.582 kb on - strand at 2258.582 kb on - strand at 2258.583 kb on - strand at 2258.691 kb on - strand at 2258.706 kb on + strand at 2258.762 kb on + strand at 2258.763 kb on - strand at 2258.763 kb on - strand at 2258.778 kb on - strand at 2258.781 kb on + strand at 2258.815 kb on - strand at 2258.898 kb on - strand at 2258.969 kb on + strand at 2258.970 kb on - strand at 2258.995 kb on - strand at 2259.009 kb on + strand, within Pf6N2E2_1906 at 2259.051 kb on - strand, within Pf6N2E2_1906 at 2259.051 kb on - strand, within Pf6N2E2_1906 at 2259.051 kb on - strand, within Pf6N2E2_1906 at 2259.076 kb on + strand, within Pf6N2E2_1906 at 2259.076 kb on + strand at 2259.077 kb on - strand, within Pf6N2E2_1906 at 2259.077 kb on - strand, within Pf6N2E2_1906 at 2259.077 kb on - strand, within Pf6N2E2_1906 at 2259.080 kb on + strand, within Pf6N2E2_1906 at 2259.080 kb on + strand, within Pf6N2E2_1906 at 2259.080 kb on + strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.081 kb on - strand, within Pf6N2E2_1906 at 2259.186 kb on + strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.187 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.308 kb on - strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.319 kb on + strand, within Pf6N2E2_1906 at 2259.320 kb on - strand, within Pf6N2E2_1906 at 2259.320 kb on - strand, within Pf6N2E2_1906 at 2259.320 kb on - strand, within Pf6N2E2_1906 at 2259.553 kb on - strand, within Pf6N2E2_1906
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 2,257,299 + Pf6N2E2_1904 0.69 +0.3 2,257,299 + Pf6N2E2_1904 0.69 -0.1 2,257,309 + Pf6N2E2_1904 0.69 +0.8 2,257,326 + Pf6N2E2_1904 0.70 -0.3 2,257,326 + Pf6N2E2_1904 0.70 -0.6 2,257,443 + Pf6N2E2_1904 0.75 -0.3 2,257,444 - Pf6N2E2_1904 0.75 -1.1 2,257,444 - Pf6N2E2_1904 0.75 +1.5 2,257,541 + Pf6N2E2_1904 0.80 +0.9 2,257,542 - Pf6N2E2_1904 0.80 -1.3 2,257,584 + Pf6N2E2_1904 0.81 -0.0 2,257,584 + Pf6N2E2_1904 0.81 +0.4 2,257,584 + Pf6N2E2_1904 0.81 -1.1 2,257,585 - Pf6N2E2_1904 0.81 +0.6 2,257,585 - Pf6N2E2_1904 0.81 +0.5 2,257,585 - Pf6N2E2_1904 0.81 +1.4 2,257,691 + Pf6N2E2_1904 0.86 -0.8 2,257,692 - Pf6N2E2_1904 0.86 +0.6 2,257,692 - Pf6N2E2_1904 0.86 +0.5 2,257,692 - Pf6N2E2_1904 0.86 +0.3 2,257,754 - Pf6N2E2_1904 0.89 +0.7 2,258,002 + +0.5 2,258,002 + +1.7 2,258,002 + -1.2 2,258,003 - +0.5 2,258,003 - -1.5 2,258,064 - +1.0 2,258,174 - +0.3 2,258,174 - +0.6 2,258,175 + +0.1 2,258,175 + -0.1 2,258,176 - +0.4 2,258,176 - +0.7 2,258,475 + Pf6N2E2_1905 0.77 -0.9 2,258,475 + Pf6N2E2_1905 0.77 -0.1 2,258,476 - Pf6N2E2_1905 0.77 -0.9 2,258,476 - Pf6N2E2_1905 0.77 -0.6 2,258,476 - Pf6N2E2_1905 0.77 +0.1 2,258,516 + Pf6N2E2_1905 0.86 -0.5 2,258,516 + Pf6N2E2_1905 0.86 -0.4 2,258,528 - Pf6N2E2_1905 0.89 -0.3 2,258,550 + -0.3 2,258,550 + -0.5 2,258,551 - -0.5 2,258,551 - +1.6 2,258,551 - -1.3 2,258,564 + -0.4 2,258,581 + +0.0 2,258,582 - -0.1 2,258,582 - +0.8 2,258,582 - +0.3 2,258,582 - -1.1 2,258,583 - -1.1 2,258,691 - -0.1 2,258,706 + +0.5 2,258,762 + +0.0 2,258,763 - +0.5 2,258,763 - -0.2 2,258,778 - +1.1 2,258,781 + -0.6 2,258,815 - +0.5 2,258,898 - -0.6 2,258,969 + -1.5 2,258,970 - -0.2 2,258,995 - +0.5 2,259,009 + Pf6N2E2_1906 0.11 -2.1 2,259,051 - Pf6N2E2_1906 0.14 +0.2 2,259,051 - Pf6N2E2_1906 0.14 -0.4 2,259,051 - Pf6N2E2_1906 0.14 -0.9 2,259,076 + Pf6N2E2_1906 0.15 -2.8 2,259,076 + +0.9 2,259,077 - Pf6N2E2_1906 0.16 -0.9 2,259,077 - Pf6N2E2_1906 0.16 -0.5 2,259,077 - Pf6N2E2_1906 0.16 -0.1 2,259,080 + Pf6N2E2_1906 0.16 +0.1 2,259,080 + Pf6N2E2_1906 0.16 +0.4 2,259,080 + Pf6N2E2_1906 0.16 +0.7 2,259,081 - Pf6N2E2_1906 0.16 -0.1 2,259,081 - Pf6N2E2_1906 0.16 +0.9 2,259,081 - Pf6N2E2_1906 0.16 -0.7 2,259,081 - Pf6N2E2_1906 0.16 -0.5 2,259,081 - Pf6N2E2_1906 0.16 +0.2 2,259,186 + Pf6N2E2_1906 0.23 -0.5 2,259,187 - Pf6N2E2_1906 0.23 -1.5 2,259,187 - Pf6N2E2_1906 0.23 -0.5 2,259,187 - Pf6N2E2_1906 0.23 +0.5 2,259,187 - Pf6N2E2_1906 0.23 -0.8 2,259,308 - Pf6N2E2_1906 0.31 -0.5 2,259,308 - Pf6N2E2_1906 0.31 -1.1 2,259,308 - Pf6N2E2_1906 0.31 -0.7 2,259,308 - Pf6N2E2_1906 0.31 +0.2 2,259,308 - Pf6N2E2_1906 0.31 +1.6 2,259,308 - Pf6N2E2_1906 0.31 +0.2 2,259,308 - Pf6N2E2_1906 0.31 +0.3 2,259,308 - Pf6N2E2_1906 0.31 -1.4 2,259,308 - Pf6N2E2_1906 0.31 +0.3 2,259,308 - Pf6N2E2_1906 0.31 +0.9 2,259,319 + Pf6N2E2_1906 0.32 +0.1 2,259,319 + Pf6N2E2_1906 0.32 +0.6 2,259,319 + Pf6N2E2_1906 0.32 -0.6 2,259,319 + Pf6N2E2_1906 0.32 -0.8 2,259,319 + Pf6N2E2_1906 0.32 -0.6 2,259,319 + Pf6N2E2_1906 0.32 -0.1 2,259,319 + Pf6N2E2_1906 0.32 +0.7 2,259,319 + Pf6N2E2_1906 0.32 +1.1 2,259,319 + Pf6N2E2_1906 0.32 -0.9 2,259,320 - Pf6N2E2_1906 0.32 -1.0 2,259,320 - Pf6N2E2_1906 0.32 +0.5 2,259,320 - Pf6N2E2_1906 0.32 +0.7 2,259,553 - Pf6N2E2_1906 0.48 -0.5
Or see this region's nucleotide sequence