Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotides Pf6N2E2_1835 and Pf6N2E2_1836 are separated by 170 nucleotides Pf6N2E2_1836 and Pf6N2E2_1837 are separated by 6 nucleotides
Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441
_1834
Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049
_1835
Pf6N2E2_1836 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,174,220 to 2,175,422
_1836
Pf6N2E2_1837 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,175,429 to 2,176,907
_1837
Position (kb)
2174
2175
2176 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2173.239 kb on - strand, within Pf6N2E2_1834 at 2173.399 kb on + strand at 2173.442 kb on - strand at 2173.442 kb on - strand at 2173.448 kb on + strand at 2173.675 kb on + strand, within Pf6N2E2_1835 at 2173.676 kb on - strand, within Pf6N2E2_1835 at 2173.726 kb on + strand, within Pf6N2E2_1835 at 2173.730 kb on + strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.731 kb on - strand, within Pf6N2E2_1835 at 2173.783 kb on + strand, within Pf6N2E2_1835 at 2173.783 kb on + strand, within Pf6N2E2_1835 at 2173.784 kb on - strand, within Pf6N2E2_1835 at 2173.927 kb on + strand, within Pf6N2E2_1835 at 2174.131 kb on + strand at 2174.132 kb on - strand at 2174.132 kb on - strand at 2174.340 kb on + strand at 2174.632 kb on + strand, within Pf6N2E2_1836 at 2174.632 kb on + strand, within Pf6N2E2_1836 at 2174.632 kb on + strand, within Pf6N2E2_1836 at 2174.633 kb on - strand, within Pf6N2E2_1836 at 2175.023 kb on - strand, within Pf6N2E2_1836 at 2175.023 kb on - strand, within Pf6N2E2_1836 at 2175.023 kb on - strand, within Pf6N2E2_1836 at 2175.023 kb on - strand, within Pf6N2E2_1836 at 2175.023 kb on - strand, within Pf6N2E2_1836 at 2175.189 kb on - strand, within Pf6N2E2_1836 at 2175.320 kb on - strand at 2175.320 kb on - strand at 2175.644 kb on + strand, within Pf6N2E2_1837 at 2175.645 kb on - strand, within Pf6N2E2_1837 at 2175.646 kb on + strand, within Pf6N2E2_1837 at 2175.646 kb on + strand, within Pf6N2E2_1837 at 2175.646 kb on + strand, within Pf6N2E2_1837 at 2175.746 kb on - strand, within Pf6N2E2_1837 at 2176.008 kb on + strand, within Pf6N2E2_1837 at 2176.331 kb on - strand, within Pf6N2E2_1837
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 2,173,239 - Pf6N2E2_1834 0.74 +0.2 2,173,399 + -1.0 2,173,442 - +0.2 2,173,442 - -1.5 2,173,448 + +1.5 2,173,675 + Pf6N2E2_1835 0.39 +0.5 2,173,676 - Pf6N2E2_1835 0.39 -1.6 2,173,726 + Pf6N2E2_1835 0.47 +0.6 2,173,730 + Pf6N2E2_1835 0.48 +0.4 2,173,731 - Pf6N2E2_1835 0.48 +0.4 2,173,731 - Pf6N2E2_1835 0.48 -0.2 2,173,731 - Pf6N2E2_1835 0.48 +0.2 2,173,783 + Pf6N2E2_1835 0.56 +0.3 2,173,783 + Pf6N2E2_1835 0.56 -3.0 2,173,784 - Pf6N2E2_1835 0.57 -0.9 2,173,927 + Pf6N2E2_1835 0.80 +0.9 2,174,131 + -0.6 2,174,132 - -0.9 2,174,132 - +0.3 2,174,340 + -1.1 2,174,632 + Pf6N2E2_1836 0.34 +0.6 2,174,632 + Pf6N2E2_1836 0.34 -0.4 2,174,632 + Pf6N2E2_1836 0.34 -3.3 2,174,633 - Pf6N2E2_1836 0.34 +0.7 2,175,023 - Pf6N2E2_1836 0.67 -0.9 2,175,023 - Pf6N2E2_1836 0.67 -0.7 2,175,023 - Pf6N2E2_1836 0.67 -0.9 2,175,023 - Pf6N2E2_1836 0.67 -1.1 2,175,023 - Pf6N2E2_1836 0.67 -1.1 2,175,189 - Pf6N2E2_1836 0.81 +0.4 2,175,320 - -1.5 2,175,320 - -0.0 2,175,644 + Pf6N2E2_1837 0.15 -0.1 2,175,645 - Pf6N2E2_1837 0.15 +0.6 2,175,646 + Pf6N2E2_1837 0.15 +0.4 2,175,646 + Pf6N2E2_1837 0.15 +0.6 2,175,646 + Pf6N2E2_1837 0.15 +0.3 2,175,746 - Pf6N2E2_1837 0.21 +0.7 2,176,008 + Pf6N2E2_1837 0.39 -0.1 2,176,331 - Pf6N2E2_1837 0.61 +0.9
Or see this region's nucleotide sequence