Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1836

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotidesPf6N2E2_1835 and Pf6N2E2_1836 are separated by 170 nucleotidesPf6N2E2_1836 and Pf6N2E2_1837 are separated by 6 nucleotides Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441 _1834 Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049 _1835 Pf6N2E2_1836 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,174,220 to 2,175,422 _1836 Pf6N2E2_1837 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,175,429 to 2,176,907 _1837 Position (kb) 2174 2175 2176Strain fitness (log2 ratio) -3 -2 -1 0 1at 2173.239 kb on - strand, within Pf6N2E2_1834at 2173.399 kb on + strandat 2173.442 kb on - strandat 2173.442 kb on - strandat 2173.448 kb on + strandat 2173.675 kb on + strand, within Pf6N2E2_1835at 2173.676 kb on - strand, within Pf6N2E2_1835at 2173.726 kb on + strand, within Pf6N2E2_1835at 2173.730 kb on + strand, within Pf6N2E2_1835at 2173.731 kb on - strand, within Pf6N2E2_1835at 2173.731 kb on - strand, within Pf6N2E2_1835at 2173.731 kb on - strand, within Pf6N2E2_1835at 2173.783 kb on + strand, within Pf6N2E2_1835at 2173.783 kb on + strand, within Pf6N2E2_1835at 2173.784 kb on - strand, within Pf6N2E2_1835at 2173.927 kb on + strand, within Pf6N2E2_1835at 2174.131 kb on + strandat 2174.132 kb on - strandat 2174.132 kb on - strandat 2174.340 kb on + strandat 2174.632 kb on + strand, within Pf6N2E2_1836at 2174.632 kb on + strand, within Pf6N2E2_1836at 2174.632 kb on + strand, within Pf6N2E2_1836at 2174.633 kb on - strand, within Pf6N2E2_1836at 2175.023 kb on - strand, within Pf6N2E2_1836at 2175.023 kb on - strand, within Pf6N2E2_1836at 2175.023 kb on - strand, within Pf6N2E2_1836at 2175.023 kb on - strand, within Pf6N2E2_1836at 2175.023 kb on - strand, within Pf6N2E2_1836at 2175.189 kb on - strand, within Pf6N2E2_1836at 2175.320 kb on - strandat 2175.320 kb on - strandat 2175.644 kb on + strand, within Pf6N2E2_1837at 2175.645 kb on - strand, within Pf6N2E2_1837at 2175.646 kb on + strand, within Pf6N2E2_1837at 2175.646 kb on + strand, within Pf6N2E2_1837at 2175.646 kb on + strand, within Pf6N2E2_1837at 2175.746 kb on - strand, within Pf6N2E2_1837at 2176.008 kb on + strand, within Pf6N2E2_1837at 2176.331 kb on - strand, within Pf6N2E2_1837

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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2,173,239 - Pf6N2E2_1834 0.74 +0.2
2,173,399 + -1.0
2,173,442 - +0.2
2,173,442 - -1.5
2,173,448 + +1.5
2,173,675 + Pf6N2E2_1835 0.39 +0.5
2,173,676 - Pf6N2E2_1835 0.39 -1.6
2,173,726 + Pf6N2E2_1835 0.47 +0.6
2,173,730 + Pf6N2E2_1835 0.48 +0.4
2,173,731 - Pf6N2E2_1835 0.48 +0.4
2,173,731 - Pf6N2E2_1835 0.48 -0.2
2,173,731 - Pf6N2E2_1835 0.48 +0.2
2,173,783 + Pf6N2E2_1835 0.56 +0.3
2,173,783 + Pf6N2E2_1835 0.56 -3.0
2,173,784 - Pf6N2E2_1835 0.57 -0.9
2,173,927 + Pf6N2E2_1835 0.80 +0.9
2,174,131 + -0.6
2,174,132 - -0.9
2,174,132 - +0.3
2,174,340 + -1.1
2,174,632 + Pf6N2E2_1836 0.34 +0.6
2,174,632 + Pf6N2E2_1836 0.34 -0.4
2,174,632 + Pf6N2E2_1836 0.34 -3.3
2,174,633 - Pf6N2E2_1836 0.34 +0.7
2,175,023 - Pf6N2E2_1836 0.67 -0.9
2,175,023 - Pf6N2E2_1836 0.67 -0.7
2,175,023 - Pf6N2E2_1836 0.67 -0.9
2,175,023 - Pf6N2E2_1836 0.67 -1.1
2,175,023 - Pf6N2E2_1836 0.67 -1.1
2,175,189 - Pf6N2E2_1836 0.81 +0.4
2,175,320 - -1.5
2,175,320 - -0.0
2,175,644 + Pf6N2E2_1837 0.15 -0.1
2,175,645 - Pf6N2E2_1837 0.15 +0.6
2,175,646 + Pf6N2E2_1837 0.15 +0.4
2,175,646 + Pf6N2E2_1837 0.15 +0.6
2,175,646 + Pf6N2E2_1837 0.15 +0.3
2,175,746 - Pf6N2E2_1837 0.21 +0.7
2,176,008 + Pf6N2E2_1837 0.39 -0.1
2,176,331 - Pf6N2E2_1837 0.61 +0.9

Or see this region's nucleotide sequence