Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1615 and Pf6N2E2_1616 are separated by 122 nucleotides Pf6N2E2_1616 and Pf6N2E2_1617 are separated by 1 nucleotides Pf6N2E2_1617 and Pf6N2E2_1618 are separated by 25 nucleotides Pf6N2E2_1618 and Pf6N2E2_1619 are separated by 44 nucleotides
Pf6N2E2_1615 - Aldo-keto reductase, at 1,888,762 to 1,889,757
_1615
Pf6N2E2_1616 - hypothetical protein, at 1,889,880 to 1,890,638
_1616
Pf6N2E2_1617 - COG2199: FOG: GGDEF domain, at 1,890,640 to 1,891,980
_1617
Pf6N2E2_1618 - ABC-type amino acid transport, signal transduction systems, periplasmic component/domain, at 1,892,006 to 1,892,740
_1618
Pf6N2E2_1619 - Glutathione S-transferase (EC 2.5.1.18), at 1,892,785 to 1,893,624
_1619
Position (kb)
1890
1891
1892 Strain fitness (log2 ratio)
-2
-1
0
1 at 1889.830 kb on + strand at 1889.830 kb on + strand at 1889.831 kb on - strand at 1889.831 kb on - strand at 1889.833 kb on + strand at 1889.833 kb on + strand at 1889.833 kb on + strand at 1889.834 kb on - strand at 1889.834 kb on - strand at 1889.834 kb on - strand at 1889.866 kb on + strand at 1889.867 kb on - strand at 1889.871 kb on + strand at 1890.078 kb on - strand, within Pf6N2E2_1616 at 1890.142 kb on - strand, within Pf6N2E2_1616 at 1890.213 kb on + strand, within Pf6N2E2_1616 at 1890.214 kb on - strand, within Pf6N2E2_1616 at 1890.214 kb on - strand, within Pf6N2E2_1616 at 1890.214 kb on - strand, within Pf6N2E2_1616 at 1890.219 kb on + strand, within Pf6N2E2_1616 at 1890.219 kb on + strand, within Pf6N2E2_1616 at 1890.220 kb on - strand, within Pf6N2E2_1616 at 1890.312 kb on + strand, within Pf6N2E2_1616 at 1890.312 kb on + strand, within Pf6N2E2_1616 at 1890.366 kb on + strand, within Pf6N2E2_1616 at 1890.379 kb on - strand, within Pf6N2E2_1616 at 1890.438 kb on + strand, within Pf6N2E2_1616 at 1890.438 kb on + strand, within Pf6N2E2_1616 at 1890.439 kb on - strand, within Pf6N2E2_1616 at 1890.505 kb on + strand, within Pf6N2E2_1616 at 1890.505 kb on + strand, within Pf6N2E2_1616 at 1890.546 kb on - strand, within Pf6N2E2_1616 at 1890.546 kb on - strand, within Pf6N2E2_1616 at 1890.610 kb on - strand at 1890.610 kb on - strand at 1890.610 kb on - strand at 1890.634 kb on + strand at 1890.635 kb on - strand at 1890.714 kb on - strand at 1891.523 kb on - strand, within Pf6N2E2_1617 at 1891.860 kb on - strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.003 kb on + strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.004 kb on - strand at 1892.005 kb on + strand at 1892.005 kb on + strand at 1892.005 kb on + strand at 1892.006 kb on - strand at 1892.006 kb on - strand at 1892.006 kb on - strand at 1892.006 kb on - strand at 1892.041 kb on - strand at 1892.105 kb on + strand, within Pf6N2E2_1618 at 1892.106 kb on - strand, within Pf6N2E2_1618 at 1892.112 kb on - strand, within Pf6N2E2_1618 at 1892.246 kb on - strand, within Pf6N2E2_1618 at 1892.292 kb on + strand, within Pf6N2E2_1618 at 1892.293 kb on - strand, within Pf6N2E2_1618 at 1892.313 kb on + strand, within Pf6N2E2_1618 at 1892.337 kb on - strand, within Pf6N2E2_1618 at 1892.353 kb on + strand, within Pf6N2E2_1618 at 1892.354 kb on - strand, within Pf6N2E2_1618 at 1892.394 kb on - strand, within Pf6N2E2_1618 at 1892.394 kb on - strand, within Pf6N2E2_1618 at 1892.433 kb on - strand, within Pf6N2E2_1618 at 1892.597 kb on + strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.598 kb on - strand, within Pf6N2E2_1618 at 1892.801 kb on - strand at 1892.926 kb on + strand, within Pf6N2E2_1619 at 1892.927 kb on - strand, within Pf6N2E2_1619
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 1,889,830 + -1.5 1,889,830 + +0.3 1,889,831 - -1.3 1,889,831 - -0.6 1,889,833 + +0.8 1,889,833 + +1.0 1,889,833 + -0.1 1,889,834 - +0.2 1,889,834 - +0.1 1,889,834 - +0.1 1,889,866 + -0.7 1,889,867 - -0.9 1,889,871 + +0.1 1,890,078 - Pf6N2E2_1616 0.26 +0.5 1,890,142 - Pf6N2E2_1616 0.35 -0.4 1,890,213 + Pf6N2E2_1616 0.44 +0.3 1,890,214 - Pf6N2E2_1616 0.44 +0.7 1,890,214 - Pf6N2E2_1616 0.44 -0.4 1,890,214 - Pf6N2E2_1616 0.44 -0.1 1,890,219 + Pf6N2E2_1616 0.45 +0.1 1,890,219 + Pf6N2E2_1616 0.45 -0.1 1,890,220 - Pf6N2E2_1616 0.45 +1.2 1,890,312 + Pf6N2E2_1616 0.57 -1.2 1,890,312 + Pf6N2E2_1616 0.57 -0.8 1,890,366 + Pf6N2E2_1616 0.64 -0.4 1,890,379 - Pf6N2E2_1616 0.66 +0.4 1,890,438 + Pf6N2E2_1616 0.74 +0.2 1,890,438 + Pf6N2E2_1616 0.74 +0.3 1,890,439 - Pf6N2E2_1616 0.74 +0.6 1,890,505 + Pf6N2E2_1616 0.82 +0.0 1,890,505 + Pf6N2E2_1616 0.82 +0.6 1,890,546 - Pf6N2E2_1616 0.88 +0.3 1,890,546 - Pf6N2E2_1616 0.88 +0.4 1,890,610 - -1.2 1,890,610 - +0.9 1,890,610 - +0.7 1,890,634 + +0.7 1,890,635 - -0.1 1,890,714 - +0.5 1,891,523 - Pf6N2E2_1617 0.66 -0.9 1,891,860 - -0.5 1,892,003 + +0.6 1,892,003 + +0.9 1,892,003 + -0.4 1,892,003 + -0.1 1,892,003 + +0.3 1,892,004 - -0.2 1,892,004 - -0.0 1,892,004 - -0.3 1,892,004 - +0.3 1,892,004 - +0.8 1,892,005 + +0.4 1,892,005 + -2.7 1,892,005 + -1.4 1,892,006 - -0.4 1,892,006 - -0.2 1,892,006 - +1.6 1,892,006 - +0.0 1,892,041 - +0.2 1,892,105 + Pf6N2E2_1618 0.13 +1.2 1,892,106 - Pf6N2E2_1618 0.14 -1.2 1,892,112 - Pf6N2E2_1618 0.14 +0.8 1,892,246 - Pf6N2E2_1618 0.33 +0.9 1,892,292 + Pf6N2E2_1618 0.39 +0.0 1,892,293 - Pf6N2E2_1618 0.39 +0.9 1,892,313 + Pf6N2E2_1618 0.42 -0.4 1,892,337 - Pf6N2E2_1618 0.45 +0.5 1,892,353 + Pf6N2E2_1618 0.47 +0.4 1,892,354 - Pf6N2E2_1618 0.47 +0.1 1,892,394 - Pf6N2E2_1618 0.53 -0.3 1,892,394 - Pf6N2E2_1618 0.53 +0.5 1,892,433 - Pf6N2E2_1618 0.58 -1.4 1,892,597 + Pf6N2E2_1618 0.80 -1.1 1,892,598 - Pf6N2E2_1618 0.81 +0.2 1,892,598 - Pf6N2E2_1618 0.81 +0.3 1,892,598 - Pf6N2E2_1618 0.81 -1.7 1,892,598 - Pf6N2E2_1618 0.81 -1.0 1,892,801 - -0.6 1,892,926 + Pf6N2E2_1619 0.17 -0.3 1,892,927 - Pf6N2E2_1619 0.17 +0.1
Or see this region's nucleotide sequence