Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1492

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1491 and Pf6N2E2_1492 are separated by 45 nucleotidesPf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotidesPf6N2E2_1493 and Pf6N2E2_1494 overlap by 14 nucleotides Pf6N2E2_1491 - Cystathionine beta-lyase (EC 4.4.1.8), at 1,743,712 to 1,746,477 _1491 Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122 _1492 Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986 _1493 Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761 _1494 Position (kb) 1746 1747 1748Strain fitness (log2 ratio) -2 -1 0 1 2at 1745.534 kb on + strand, within Pf6N2E2_1491at 1745.534 kb on + strand, within Pf6N2E2_1491at 1745.534 kb on + strand, within Pf6N2E2_1491at 1745.535 kb on - strand, within Pf6N2E2_1491at 1745.535 kb on - strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.840 kb on + strand, within Pf6N2E2_1491at 1745.841 kb on - strand, within Pf6N2E2_1491at 1745.841 kb on - strand, within Pf6N2E2_1491at 1745.841 kb on - strand, within Pf6N2E2_1491at 1746.118 kb on + strand, within Pf6N2E2_1491at 1746.119 kb on - strand, within Pf6N2E2_1491at 1746.119 kb on - strand, within Pf6N2E2_1491at 1746.346 kb on - strandat 1746.463 kb on + strandat 1746.464 kb on - strandat 1746.534 kb on - strandat 1746.569 kb on + strandat 1746.570 kb on - strandat 1746.636 kb on - strand, within Pf6N2E2_1492at 1746.715 kb on - strand, within Pf6N2E2_1492at 1746.715 kb on - strand, within Pf6N2E2_1492at 1746.758 kb on + strand, within Pf6N2E2_1492at 1746.792 kb on - strand, within Pf6N2E2_1492at 1747.117 kb on - strandat 1747.191 kb on - strandat 1747.191 kb on - strandat 1747.191 kb on - strandat 1747.243 kb on + strand, within Pf6N2E2_1493at 1747.332 kb on - strand, within Pf6N2E2_1493at 1747.382 kb on - strand, within Pf6N2E2_1493at 1747.694 kb on + strand, within Pf6N2E2_1493at 1747.694 kb on + strand, within Pf6N2E2_1493at 1747.694 kb on + strand, within Pf6N2E2_1493at 1747.694 kb on + strand, within Pf6N2E2_1493at 1747.695 kb on - strand, within Pf6N2E2_1493at 1747.695 kb on - strand, within Pf6N2E2_1493at 1747.977 kb on + strandat 1748.008 kb on + strandat 1748.009 kb on - strandat 1748.031 kb on + strandat 1748.062 kb on - strand, within Pf6N2E2_1494at 1748.062 kb on - strand, within Pf6N2E2_1494

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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1,745,534 + Pf6N2E2_1491 0.66 +0.3
1,745,534 + Pf6N2E2_1491 0.66 -0.4
1,745,534 + Pf6N2E2_1491 0.66 +0.4
1,745,535 - Pf6N2E2_1491 0.66 +0.0
1,745,535 - Pf6N2E2_1491 0.66 -0.2
1,745,840 + Pf6N2E2_1491 0.77 +1.0
1,745,840 + Pf6N2E2_1491 0.77 -0.3
1,745,840 + Pf6N2E2_1491 0.77 +0.3
1,745,840 + Pf6N2E2_1491 0.77 +1.3
1,745,841 - Pf6N2E2_1491 0.77 +0.2
1,745,841 - Pf6N2E2_1491 0.77 +0.1
1,745,841 - Pf6N2E2_1491 0.77 +0.8
1,746,118 + Pf6N2E2_1491 0.87 +0.4
1,746,119 - Pf6N2E2_1491 0.87 +0.6
1,746,119 - Pf6N2E2_1491 0.87 +0.7
1,746,346 - -0.5
1,746,463 + -0.9
1,746,464 - +0.0
1,746,534 - +0.3
1,746,569 + -0.1
1,746,570 - -0.2
1,746,636 - Pf6N2E2_1492 0.19 +0.7
1,746,715 - Pf6N2E2_1492 0.32 -0.3
1,746,715 - Pf6N2E2_1492 0.32 +0.1
1,746,758 + Pf6N2E2_1492 0.39 -0.9
1,746,792 - Pf6N2E2_1492 0.45 -2.1
1,747,117 - -0.2
1,747,191 - -0.1
1,747,191 - -0.2
1,747,191 - -0.6
1,747,243 + Pf6N2E2_1493 0.11 -0.8
1,747,332 - Pf6N2E2_1493 0.22 +1.5
1,747,382 - Pf6N2E2_1493 0.28 -0.0
1,747,694 + Pf6N2E2_1493 0.65 -1.2
1,747,694 + Pf6N2E2_1493 0.65 +0.1
1,747,694 + Pf6N2E2_1493 0.65 +1.9
1,747,694 + Pf6N2E2_1493 0.65 +0.6
1,747,695 - Pf6N2E2_1493 0.65 -0.4
1,747,695 - Pf6N2E2_1493 0.65 -0.6
1,747,977 + +0.3
1,748,008 + -0.0
1,748,009 - +0.7
1,748,031 + +0.4
1,748,062 - Pf6N2E2_1494 0.11 +0.1
1,748,062 - Pf6N2E2_1494 0.11 -0.4

Or see this region's nucleotide sequence