Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1435 and Pf6N2E2_1436 are separated by 40 nucleotides Pf6N2E2_1436 and Pf6N2E2_1437 are separated by 377 nucleotides Pf6N2E2_1437 and Pf6N2E2_1438 are separated by 527 nucleotides
Pf6N2E2_1435 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 1,684,697 to 1,685,794
_1435
Pf6N2E2_1436 - FMN reductase (EC 1.5.1.29), at 1,685,835 to 1,686,398
_1436
Pf6N2E2_1437 - Acyl-CoA dehydrogenase family protein, at 1,686,776 to 1,688,020
_1437
Pf6N2E2_1438 - Methyltransferase (EC 2.1.1.-), at 1,688,548 to 1,689,132
_1438
Position (kb)
1686
1687
1688
1689 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.795 kb on + strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.796 kb on - strand at 1685.801 kb on + strand at 1685.802 kb on - strand at 1685.802 kb on - strand at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1685.965 kb on + strand, within Pf6N2E2_1436 at 1686.079 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.137 kb on - strand, within Pf6N2E2_1436 at 1686.149 kb on - strand, within Pf6N2E2_1436 at 1686.447 kb on - strand at 1686.483 kb on - strand at 1687.254 kb on - strand, within Pf6N2E2_1437 at 1687.256 kb on + strand, within Pf6N2E2_1437 at 1687.256 kb on + strand, within Pf6N2E2_1437 at 1687.257 kb on - strand, within Pf6N2E2_1437 at 1687.270 kb on + strand, within Pf6N2E2_1437 at 1687.295 kb on + strand, within Pf6N2E2_1437 at 1687.295 kb on + strand, within Pf6N2E2_1437 at 1687.344 kb on + strand, within Pf6N2E2_1437 at 1687.662 kb on - strand, within Pf6N2E2_1437 at 1687.712 kb on + strand, within Pf6N2E2_1437 at 1687.931 kb on + strand at 1687.931 kb on + strand at 1687.931 kb on + strand at 1687.932 kb on - strand at 1687.982 kb on + strand at 1687.983 kb on - strand at 1688.022 kb on + strand at 1688.022 kb on + strand at 1688.066 kb on + strand at 1688.067 kb on - strand at 1688.215 kb on + strand at 1688.216 kb on - strand at 1688.216 kb on - strand at 1688.282 kb on - strand at 1688.282 kb on - strand at 1688.298 kb on - strand at 1688.323 kb on - strand at 1688.323 kb on - strand at 1688.331 kb on + strand at 1688.332 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.391 kb on - strand at 1688.396 kb on - strand at 1688.416 kb on + strand at 1688.416 kb on + strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.417 kb on - strand at 1688.450 kb on + strand at 1688.507 kb on + strand at 1688.508 kb on - strand at 1688.744 kb on + strand, within Pf6N2E2_1438 at 1688.745 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.821 kb on - strand, within Pf6N2E2_1438 at 1688.930 kb on - strand, within Pf6N2E2_1438 at 1688.988 kb on + strand, within Pf6N2E2_1438 at 1688.989 kb on - strand, within Pf6N2E2_1438
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 1,685,795 + +0.0 1,685,795 + +1.6 1,685,795 + -0.8 1,685,795 + -0.3 1,685,795 + +0.1 1,685,795 + -0.3 1,685,795 + -0.1 1,685,795 + +1.0 1,685,795 + +0.4 1,685,795 + +0.6 1,685,795 + +0.7 1,685,795 + +0.4 1,685,795 + +1.5 1,685,796 - +0.1 1,685,796 - +0.5 1,685,796 - +0.4 1,685,796 - +1.8 1,685,796 - -1.1 1,685,796 - +0.2 1,685,796 - +0.3 1,685,801 + +0.7 1,685,802 - -0.2 1,685,802 - -2.7 1,685,965 + Pf6N2E2_1436 0.23 +1.7 1,685,965 + Pf6N2E2_1436 0.23 +0.2 1,685,965 + Pf6N2E2_1436 0.23 +0.3 1,685,965 + Pf6N2E2_1436 0.23 +0.3 1,685,965 + Pf6N2E2_1436 0.23 -0.1 1,686,079 - Pf6N2E2_1436 0.43 +0.2 1,686,137 - Pf6N2E2_1436 0.54 -1.3 1,686,137 - Pf6N2E2_1436 0.54 +0.0 1,686,149 - Pf6N2E2_1436 0.56 -0.1 1,686,447 - +0.2 1,686,483 - +0.3 1,687,254 - Pf6N2E2_1437 0.38 -1.7 1,687,256 + Pf6N2E2_1437 0.39 -1.3 1,687,256 + Pf6N2E2_1437 0.39 -0.3 1,687,257 - Pf6N2E2_1437 0.39 -1.9 1,687,270 + Pf6N2E2_1437 0.40 +0.5 1,687,295 + Pf6N2E2_1437 0.42 +0.1 1,687,295 + Pf6N2E2_1437 0.42 -1.8 1,687,344 + Pf6N2E2_1437 0.46 -0.3 1,687,662 - Pf6N2E2_1437 0.71 -1.1 1,687,712 + Pf6N2E2_1437 0.75 -0.9 1,687,931 + -0.0 1,687,931 + +0.5 1,687,931 + +0.8 1,687,932 - -0.2 1,687,982 + +0.3 1,687,983 - +0.1 1,688,022 + -0.8 1,688,022 + +1.8 1,688,066 + +0.3 1,688,067 - -0.2 1,688,215 + +0.7 1,688,216 - +0.2 1,688,216 - -0.1 1,688,282 - +0.9 1,688,282 - +0.2 1,688,298 - +0.1 1,688,323 - -2.8 1,688,323 - -0.8 1,688,331 + +1.0 1,688,332 - -0.6 1,688,391 - +0.3 1,688,391 - +0.1 1,688,391 - -1.1 1,688,396 - +0.7 1,688,416 + +0.3 1,688,416 + -0.0 1,688,417 - -0.9 1,688,417 - -0.3 1,688,417 - -0.5 1,688,450 + -0.0 1,688,507 + +0.7 1,688,508 - -1.8 1,688,744 + Pf6N2E2_1438 0.34 +0.8 1,688,745 - Pf6N2E2_1438 0.34 -0.0 1,688,821 - Pf6N2E2_1438 0.47 -0.1 1,688,821 - Pf6N2E2_1438 0.47 -1.0 1,688,930 - Pf6N2E2_1438 0.65 +1.1 1,688,988 + Pf6N2E2_1438 0.75 -0.4 1,688,989 - Pf6N2E2_1438 0.75 -0.6
Or see this region's nucleotide sequence