Experiment: Benzethonium chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1102 and Pf6N2E2_1103 are separated by 11 nucleotides Pf6N2E2_1103 and Pf6N2E2_1104 overlap by 4 nucleotides
Pf6N2E2_1102 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 1,288,309 to 1,289,742
_1102
Pf6N2E2_1103 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,289,754 to 1,290,554
_1103
Pf6N2E2_1104 - Gluconate dehydratase (EC 4.2.1.39), at 1,290,551 to 1,291,750
_1104
Position (kb)
1289
1290
1291 Strain fitness (log2 ratio)
-2
-1
0
1 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.408 kb on + strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.409 kb on - strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.417 kb on + strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.418 kb on - strand, within Pf6N2E2_1102 at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.670 kb on - strand at 1289.758 kb on + strand at 1289.759 kb on - strand at 1289.973 kb on + strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1290.145 kb on + strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.359 kb on - strand, within Pf6N2E2_1103 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.784 kb on - strand, within Pf6N2E2_1104 at 1290.807 kb on - strand, within Pf6N2E2_1104 at 1290.977 kb on + strand, within Pf6N2E2_1104 at 1291.083 kb on + strand, within Pf6N2E2_1104 at 1291.084 kb on - strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.426 kb on - strand, within Pf6N2E2_1104 at 1291.503 kb on + strand, within Pf6N2E2_1104
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM remove 1,288,877 + Pf6N2E2_1102 0.40 -0.0 1,288,877 + Pf6N2E2_1102 0.40 +0.4 1,288,877 + Pf6N2E2_1102 0.40 +0.8 1,289,408 + Pf6N2E2_1102 0.77 +0.6 1,289,408 + Pf6N2E2_1102 0.77 +0.8 1,289,408 + Pf6N2E2_1102 0.77 +1.2 1,289,409 - Pf6N2E2_1102 0.77 +1.4 1,289,409 - Pf6N2E2_1102 0.77 -0.5 1,289,417 + Pf6N2E2_1102 0.77 +0.9 1,289,417 + Pf6N2E2_1102 0.77 -0.8 1,289,417 + Pf6N2E2_1102 0.77 +0.9 1,289,417 + Pf6N2E2_1102 0.77 -0.4 1,289,417 + Pf6N2E2_1102 0.77 +0.4 1,289,418 - Pf6N2E2_1102 0.77 -0.2 1,289,418 - Pf6N2E2_1102 0.77 +0.7 1,289,669 + -0.0 1,289,669 + -0.4 1,289,669 + -0.5 1,289,669 + +1.0 1,289,669 + +0.9 1,289,670 - +1.1 1,289,758 + +0.4 1,289,759 - +1.5 1,289,973 + Pf6N2E2_1103 0.27 -0.3 1,289,974 - Pf6N2E2_1103 0.27 -0.8 1,289,974 - Pf6N2E2_1103 0.27 +0.3 1,290,145 + Pf6N2E2_1103 0.49 -1.5 1,290,146 - Pf6N2E2_1103 0.49 -0.8 1,290,146 - Pf6N2E2_1103 0.49 +0.1 1,290,146 - Pf6N2E2_1103 0.49 +0.3 1,290,359 - Pf6N2E2_1103 0.76 -1.7 1,290,755 - Pf6N2E2_1104 0.17 +0.5 1,290,755 - Pf6N2E2_1104 0.17 -1.5 1,290,755 - Pf6N2E2_1104 0.17 -0.7 1,290,777 + Pf6N2E2_1104 0.19 +1.0 1,290,777 + Pf6N2E2_1104 0.19 -0.3 1,290,783 + Pf6N2E2_1104 0.19 -0.6 1,290,783 + Pf6N2E2_1104 0.19 -0.9 1,290,784 - Pf6N2E2_1104 0.19 -0.4 1,290,807 - Pf6N2E2_1104 0.21 +0.2 1,290,977 + Pf6N2E2_1104 0.35 -0.2 1,291,083 + Pf6N2E2_1104 0.44 +0.4 1,291,084 - Pf6N2E2_1104 0.44 +0.7 1,291,410 + Pf6N2E2_1104 0.72 +0.7 1,291,410 + Pf6N2E2_1104 0.72 +1.6 1,291,410 + Pf6N2E2_1104 0.72 -0.8 1,291,410 + Pf6N2E2_1104 0.72 -0.4 1,291,425 + Pf6N2E2_1104 0.73 -2.3 1,291,425 + Pf6N2E2_1104 0.73 -0.9 1,291,425 + Pf6N2E2_1104 0.73 -0.3 1,291,425 + Pf6N2E2_1104 0.73 -2.1 1,291,426 - Pf6N2E2_1104 0.73 +1.6 1,291,503 + Pf6N2E2_1104 0.79 +0.2
Or see this region's nucleotide sequence