Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1074

Experiment: Benzethonium chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1073 and Pf6N2E2_1074 are separated by 229 nucleotidesPf6N2E2_1074 and Pf6N2E2_1075 are separated by 20 nucleotides Pf6N2E2_1073 - Glycogen debranching enzyme (EC 3.2.1.-), at 1,251,877 to 1,254,036 _1073 Pf6N2E2_1074 - FIG00953356: hypothetical protein, at 1,254,266 to 1,254,619 _1074 Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426 _1075 Position (kb) 1254 1255Strain fitness (log2 ratio) -2 -1 0 1 2at 1253.270 kb on + strand, within Pf6N2E2_1073at 1253.271 kb on - strand, within Pf6N2E2_1073at 1253.306 kb on + strand, within Pf6N2E2_1073at 1253.306 kb on + strand, within Pf6N2E2_1073at 1253.307 kb on - strand, within Pf6N2E2_1073at 1253.315 kb on + strand, within Pf6N2E2_1073at 1253.315 kb on + strand, within Pf6N2E2_1073at 1253.316 kb on - strand, within Pf6N2E2_1073at 1253.316 kb on - strand, within Pf6N2E2_1073at 1253.316 kb on - strand, within Pf6N2E2_1073at 1253.316 kb on - strand, within Pf6N2E2_1073at 1253.446 kb on - strandat 1253.507 kb on + strand, within Pf6N2E2_1073at 1253.507 kb on + strand, within Pf6N2E2_1073at 1253.765 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.780 kb on + strand, within Pf6N2E2_1073at 1253.781 kb on - strand, within Pf6N2E2_1073at 1253.781 kb on - strand, within Pf6N2E2_1073at 1253.781 kb on - strand, within Pf6N2E2_1073at 1253.819 kb on + strand, within Pf6N2E2_1073at 1253.834 kb on + strandat 1253.834 kb on + strandat 1254.036 kb on + strandat 1254.036 kb on + strandat 1254.036 kb on + strandat 1254.036 kb on + strandat 1254.036 kb on + strandat 1254.037 kb on - strandat 1254.037 kb on - strandat 1254.132 kb on + strandat 1254.133 kb on - strandat 1254.133 kb on - strandat 1254.560 kb on - strand, within Pf6N2E2_1074at 1254.583 kb on - strand, within Pf6N2E2_1074at 1254.850 kb on + strandat 1254.850 kb on + strandat 1254.851 kb on - strandat 1254.851 kb on - strandat 1254.851 kb on - strandat 1254.955 kb on + strand, within Pf6N2E2_1075at 1254.955 kb on + strand, within Pf6N2E2_1075at 1254.956 kb on - strand, within Pf6N2E2_1075at 1255.149 kb on - strand, within Pf6N2E2_1075at 1255.149 kb on - strand, within Pf6N2E2_1075at 1255.316 kb on + strand, within Pf6N2E2_1075at 1255.556 kb on + strand, within Pf6N2E2_1075at 1255.557 kb on - strand, within Pf6N2E2_1075

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzethonium chloride 0.02 mM
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1,253,270 + Pf6N2E2_1073 0.64 +0.5
1,253,271 - Pf6N2E2_1073 0.65 -2.1
1,253,306 + Pf6N2E2_1073 0.66 -0.1
1,253,306 + Pf6N2E2_1073 0.66 +0.7
1,253,307 - Pf6N2E2_1073 0.66 -1.0
1,253,315 + Pf6N2E2_1073 0.67 -0.0
1,253,315 + Pf6N2E2_1073 0.67 -0.7
1,253,316 - Pf6N2E2_1073 0.67 +0.8
1,253,316 - Pf6N2E2_1073 0.67 +0.1
1,253,316 - Pf6N2E2_1073 0.67 +1.5
1,253,316 - Pf6N2E2_1073 0.67 +1.2
1,253,446 - +2.3
1,253,507 + Pf6N2E2_1073 0.75 -0.7
1,253,507 + Pf6N2E2_1073 0.75 +0.2
1,253,765 + Pf6N2E2_1073 0.87 -0.4
1,253,780 + Pf6N2E2_1073 0.88 +1.1
1,253,780 + Pf6N2E2_1073 0.88 -0.2
1,253,780 + Pf6N2E2_1073 0.88 +0.0
1,253,780 + Pf6N2E2_1073 0.88 -0.6
1,253,780 + Pf6N2E2_1073 0.88 -0.8
1,253,780 + Pf6N2E2_1073 0.88 -0.3
1,253,780 + Pf6N2E2_1073 0.88 -0.3
1,253,780 + Pf6N2E2_1073 0.88 +0.2
1,253,780 + Pf6N2E2_1073 0.88 +0.1
1,253,781 - Pf6N2E2_1073 0.88 -0.4
1,253,781 - Pf6N2E2_1073 0.88 +1.2
1,253,781 - Pf6N2E2_1073 0.88 -0.5
1,253,819 + Pf6N2E2_1073 0.90 +0.3
1,253,834 + -0.5
1,253,834 + +0.2
1,254,036 + -0.1
1,254,036 + -1.1
1,254,036 + +0.5
1,254,036 + +0.1
1,254,036 + -1.3
1,254,037 - +0.5
1,254,037 - +0.8
1,254,132 + -0.2
1,254,133 - +0.1
1,254,133 - +0.9
1,254,560 - Pf6N2E2_1074 0.83 -0.8
1,254,583 - Pf6N2E2_1074 0.90 -0.2
1,254,850 + +0.5
1,254,850 + +0.2
1,254,851 - +0.6
1,254,851 - +0.0
1,254,851 - -1.2
1,254,955 + Pf6N2E2_1075 0.11 +1.4
1,254,955 + Pf6N2E2_1075 0.11 -0.2
1,254,956 - Pf6N2E2_1075 0.11 -0.3
1,255,149 - Pf6N2E2_1075 0.18 -1.1
1,255,149 - Pf6N2E2_1075 0.18 -0.1
1,255,316 + Pf6N2E2_1075 0.24 +0.3
1,255,556 + Pf6N2E2_1075 0.33 +0.2
1,255,557 - Pf6N2E2_1075 0.33 +0.2

Or see this region's nucleotide sequence