Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_888

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_887 and Pf1N1B4_888 are separated by 274 nucleotidesPf1N1B4_888 and Pf1N1B4_889 are separated by 129 nucleotidesPf1N1B4_889 and Pf1N1B4_890 are separated by 98 nucleotides Pf1N1B4_887 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 1,010,235 to 1,014,131 _887 Pf1N1B4_888 - Transglycosylase, Slt family, at 1,014,406 to 1,015,866 _888 Pf1N1B4_889 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 1,015,996 to 1,016,742 _889 Pf1N1B4_890 - DNA recombination and repair protein RecO, at 1,016,841 to 1,017,530 _890 Position (kb) 1014 1015 1016Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1013.885 kb on + strandat 1014.034 kb on + strandat 1014.178 kb on + strandat 1014.285 kb on - strandat 1014.335 kb on + strandat 1014.335 kb on + strandat 1014.336 kb on - strandat 1014.336 kb on - strandat 1014.336 kb on - strandat 1014.372 kb on - strandat 1014.394 kb on + strandat 1014.394 kb on + strandat 1014.395 kb on - strandat 1014.395 kb on - strandat 1014.396 kb on + strandat 1014.396 kb on + strandat 1014.566 kb on - strand, within Pf1N1B4_888at 1015.036 kb on + strandat 1015.036 kb on + strand, within Pf1N1B4_888at 1015.037 kb on - strand, within Pf1N1B4_888at 1015.037 kb on - strandat 1015.141 kb on + strand, within Pf1N1B4_888at 1015.141 kb on + strand, within Pf1N1B4_888at 1015.195 kb on + strandat 1015.211 kb on + strand, within Pf1N1B4_888at 1015.231 kb on + strand, within Pf1N1B4_888at 1015.232 kb on - strand, within Pf1N1B4_888at 1015.270 kb on + strandat 1015.271 kb on - strand, within Pf1N1B4_888at 1015.489 kb on + strand, within Pf1N1B4_888at 1015.489 kb on + strand, within Pf1N1B4_888at 1015.489 kb on + strand, within Pf1N1B4_888at 1015.489 kb on + strand, within Pf1N1B4_888at 1015.490 kb on - strand, within Pf1N1B4_888at 1015.533 kb on + strandat 1015.533 kb on + strand, within Pf1N1B4_888at 1015.675 kb on + strand, within Pf1N1B4_888at 1015.744 kb on + strandat 1015.747 kb on + strandat 1015.864 kb on + strandat 1015.865 kb on - strandat 1015.865 kb on - strandat 1015.865 kb on - strandat 1016.760 kb on - strandat 1016.783 kb on + strandat 1016.783 kb on + strandat 1016.783 kb on + strandat 1016.783 kb on + strandat 1016.784 kb on - strandat 1016.784 kb on - strandat 1016.784 kb on - strandat 1016.784 kb on - strandat 1016.784 kb on - strandat 1016.828 kb on - strandat 1016.843 kb on - strandat 1016.849 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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1,013,885 + -1.7
1,014,034 + -2.2
1,014,178 + -1.3
1,014,285 - +0.1
1,014,335 + +2.9
1,014,335 + +0.1
1,014,336 - -0.5
1,014,336 - +0.0
1,014,336 - -0.7
1,014,372 - -1.2
1,014,394 + +1.9
1,014,394 + -2.2
1,014,395 - +0.1
1,014,395 - +1.1
1,014,396 + -2.5
1,014,396 + -1.9
1,014,566 - Pf1N1B4_888 0.11 -1.9
1,015,036 + +0.1
1,015,036 + Pf1N1B4_888 0.43 -1.3
1,015,037 - Pf1N1B4_888 0.43 -0.1
1,015,037 - +2.1
1,015,141 + Pf1N1B4_888 0.50 -3.2
1,015,141 + Pf1N1B4_888 0.50 -2.5
1,015,195 + -1.5
1,015,211 + Pf1N1B4_888 0.55 -3.0
1,015,231 + Pf1N1B4_888 0.56 -0.9
1,015,232 - Pf1N1B4_888 0.57 +0.1
1,015,270 + +0.1
1,015,271 - Pf1N1B4_888 0.59 +0.1
1,015,489 + Pf1N1B4_888 0.74 -2.7
1,015,489 + Pf1N1B4_888 0.74 +0.1
1,015,489 + Pf1N1B4_888 0.74 -1.5
1,015,489 + Pf1N1B4_888 0.74 +0.1
1,015,490 - Pf1N1B4_888 0.74 -2.5
1,015,533 + +0.1
1,015,533 + Pf1N1B4_888 0.77 -0.5
1,015,675 + Pf1N1B4_888 0.87 -1.9
1,015,744 + +2.1
1,015,747 + -2.5
1,015,864 + -2.5
1,015,865 - -0.9
1,015,865 - -2.5
1,015,865 - -0.0
1,016,760 - +0.8
1,016,783 + -0.5
1,016,783 + +2.9
1,016,783 + -0.9
1,016,783 + +0.1
1,016,784 - -0.9
1,016,784 - -1.6
1,016,784 - -0.3
1,016,784 - -1.2
1,016,784 - -0.9
1,016,828 - +0.4
1,016,843 - +0.3
1,016,849 + -0.9

Or see this region's nucleotide sequence