Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_887 and Pf1N1B4_888 are separated by 274 nucleotides Pf1N1B4_888 and Pf1N1B4_889 are separated by 129 nucleotides Pf1N1B4_889 and Pf1N1B4_890 are separated by 98 nucleotides
Pf1N1B4_887 - Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3), at 1,010,235 to 1,014,131
_887
Pf1N1B4_888 - Transglycosylase, Slt family, at 1,014,406 to 1,015,866
_888
Pf1N1B4_889 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 1,015,996 to 1,016,742
_889
Pf1N1B4_890 - DNA recombination and repair protein RecO, at 1,016,841 to 1,017,530
_890
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1013.885 kb on + strand at 1014.034 kb on + strand at 1014.178 kb on + strand at 1014.285 kb on - strand at 1014.335 kb on + strand at 1014.335 kb on + strand at 1014.336 kb on - strand at 1014.336 kb on - strand at 1014.336 kb on - strand at 1014.372 kb on - strand at 1014.394 kb on + strand at 1014.394 kb on + strand at 1014.395 kb on - strand at 1014.395 kb on - strand at 1014.396 kb on + strand at 1014.396 kb on + strand at 1014.566 kb on - strand, within Pf1N1B4_888 at 1015.036 kb on + strand at 1015.036 kb on + strand, within Pf1N1B4_888 at 1015.037 kb on - strand, within Pf1N1B4_888 at 1015.037 kb on - strand at 1015.141 kb on + strand, within Pf1N1B4_888 at 1015.141 kb on + strand, within Pf1N1B4_888 at 1015.195 kb on + strand at 1015.211 kb on + strand, within Pf1N1B4_888 at 1015.231 kb on + strand, within Pf1N1B4_888 at 1015.232 kb on - strand, within Pf1N1B4_888 at 1015.270 kb on + strand at 1015.271 kb on - strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.489 kb on + strand, within Pf1N1B4_888 at 1015.490 kb on - strand, within Pf1N1B4_888 at 1015.533 kb on + strand at 1015.533 kb on + strand, within Pf1N1B4_888 at 1015.675 kb on + strand, within Pf1N1B4_888 at 1015.744 kb on + strand at 1015.747 kb on + strand at 1015.864 kb on + strand at 1015.865 kb on - strand at 1015.865 kb on - strand at 1015.865 kb on - strand at 1016.760 kb on - strand at 1016.783 kb on + strand at 1016.783 kb on + strand at 1016.783 kb on + strand at 1016.783 kb on + strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.784 kb on - strand at 1016.828 kb on - strand at 1016.843 kb on - strand at 1016.849 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 1,013,885 + -1.7 1,014,034 + -2.2 1,014,178 + -1.3 1,014,285 - +0.1 1,014,335 + +2.9 1,014,335 + +0.1 1,014,336 - -0.5 1,014,336 - +0.0 1,014,336 - -0.7 1,014,372 - -1.2 1,014,394 + +1.9 1,014,394 + -2.2 1,014,395 - +0.1 1,014,395 - +1.1 1,014,396 + -2.5 1,014,396 + -1.9 1,014,566 - Pf1N1B4_888 0.11 -1.9 1,015,036 + +0.1 1,015,036 + Pf1N1B4_888 0.43 -1.3 1,015,037 - Pf1N1B4_888 0.43 -0.1 1,015,037 - +2.1 1,015,141 + Pf1N1B4_888 0.50 -3.2 1,015,141 + Pf1N1B4_888 0.50 -2.5 1,015,195 + -1.5 1,015,211 + Pf1N1B4_888 0.55 -3.0 1,015,231 + Pf1N1B4_888 0.56 -0.9 1,015,232 - Pf1N1B4_888 0.57 +0.1 1,015,270 + +0.1 1,015,271 - Pf1N1B4_888 0.59 +0.1 1,015,489 + Pf1N1B4_888 0.74 -2.7 1,015,489 + Pf1N1B4_888 0.74 +0.1 1,015,489 + Pf1N1B4_888 0.74 -1.5 1,015,489 + Pf1N1B4_888 0.74 +0.1 1,015,490 - Pf1N1B4_888 0.74 -2.5 1,015,533 + +0.1 1,015,533 + Pf1N1B4_888 0.77 -0.5 1,015,675 + Pf1N1B4_888 0.87 -1.9 1,015,744 + +2.1 1,015,747 + -2.5 1,015,864 + -2.5 1,015,865 - -0.9 1,015,865 - -2.5 1,015,865 - -0.0 1,016,760 - +0.8 1,016,783 + -0.5 1,016,783 + +2.9 1,016,783 + -0.9 1,016,783 + +0.1 1,016,784 - -0.9 1,016,784 - -1.6 1,016,784 - -0.3 1,016,784 - -1.2 1,016,784 - -0.9 1,016,828 - +0.4 1,016,843 - +0.3 1,016,849 + -0.9
Or see this region's nucleotide sequence