Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_87

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_85 and Pf1N1B4_86 are separated by 29 nucleotidesPf1N1B4_86 and Pf1N1B4_87 overlap by 36 nucleotidesPf1N1B4_87 and Pf1N1B4_88 are separated by 298 nucleotidesPf1N1B4_88 and Pf1N1B4_89 are separated by 131 nucleotides Pf1N1B4_85 - Oxidoreductase, aldo/keto reductase family, at 88,514 to 89,335 _85 Pf1N1B4_86 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 89,365 to 89,835 _86 Pf1N1B4_87 - DNA polymerase II (EC 2.7.7.7), at 89,800 to 92,187 _87 Pf1N1B4_88 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 92,486 to 92,926 _88 Pf1N1B4_89 - Uncharacterized GST-like protein yncG, at 93,058 to 93,678 _89 Position (kb) 89 90 91 92 93Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 88.820 kb on + strand, within Pf1N1B4_85at 88.820 kb on + strand, within Pf1N1B4_85at 88.994 kb on + strand, within Pf1N1B4_85at 88.994 kb on + strand, within Pf1N1B4_85at 88.995 kb on - strand, within Pf1N1B4_85at 88.996 kb on + strandat 89.070 kb on - strand, within Pf1N1B4_85at 89.070 kb on - strand, within Pf1N1B4_85at 89.070 kb on - strand, within Pf1N1B4_85at 89.311 kb on + strandat 89.311 kb on + strandat 89.312 kb on - strandat 89.312 kb on - strandat 89.312 kb on - strandat 89.312 kb on - strandat 89.491 kb on - strand, within Pf1N1B4_86at 89.536 kb on + strand, within Pf1N1B4_86at 89.536 kb on + strand, within Pf1N1B4_86at 89.897 kb on + strandat 89.897 kb on + strandat 89.959 kb on + strandat 89.959 kb on + strandat 89.959 kb on + strandat 89.960 kb on - strandat 89.960 kb on - strandat 89.963 kb on + strandat 89.963 kb on + strandat 89.963 kb on + strandat 89.964 kb on - strandat 89.964 kb on - strandat 89.964 kb on - strandat 89.964 kb on - strandat 90.018 kb on - strandat 90.018 kb on - strandat 90.060 kb on + strand, within Pf1N1B4_87at 90.061 kb on - strand, within Pf1N1B4_87at 90.061 kb on - strand, within Pf1N1B4_87at 90.068 kb on + strand, within Pf1N1B4_87at 90.146 kb on + strand, within Pf1N1B4_87at 90.147 kb on - strand, within Pf1N1B4_87at 90.158 kb on + strand, within Pf1N1B4_87at 90.194 kb on + strand, within Pf1N1B4_87at 90.194 kb on + strand, within Pf1N1B4_87at 90.195 kb on - strand, within Pf1N1B4_87at 90.261 kb on - strand, within Pf1N1B4_87at 90.363 kb on - strand, within Pf1N1B4_87at 90.363 kb on - strand, within Pf1N1B4_87at 90.530 kb on + strand, within Pf1N1B4_87at 90.530 kb on + strand, within Pf1N1B4_87at 90.659 kb on + strand, within Pf1N1B4_87at 90.659 kb on + strand, within Pf1N1B4_87at 90.659 kb on + strand, within Pf1N1B4_87at 90.660 kb on - strand, within Pf1N1B4_87at 90.660 kb on - strand, within Pf1N1B4_87at 90.660 kb on - strand, within Pf1N1B4_87at 91.026 kb on + strand, within Pf1N1B4_87at 91.026 kb on + strand, within Pf1N1B4_87at 91.026 kb on + strand, within Pf1N1B4_87at 91.027 kb on - strand, within Pf1N1B4_87at 91.028 kb on + strand, within Pf1N1B4_87at 91.028 kb on + strand, within Pf1N1B4_87at 91.028 kb on + strand, within Pf1N1B4_87at 91.028 kb on + strand, within Pf1N1B4_87at 91.029 kb on - strandat 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strand, within Pf1N1B4_87at 91.029 kb on - strandat 91.029 kb on - strand, within Pf1N1B4_87at 91.166 kb on + strand, within Pf1N1B4_87at 91.166 kb on + strand, within Pf1N1B4_87at 91.167 kb on - strandat 91.364 kb on + strand, within Pf1N1B4_87at 91.364 kb on + strandat 91.365 kb on - strand, within Pf1N1B4_87at 91.365 kb on - strand, within Pf1N1B4_87at 91.365 kb on - strand, within Pf1N1B4_87at 91.365 kb on - strand, within Pf1N1B4_87at 91.565 kb on + strand, within Pf1N1B4_87at 91.566 kb on - strand, within Pf1N1B4_87at 91.619 kb on + strandat 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.619 kb on + strand, within Pf1N1B4_87at 91.620 kb on - strand, within Pf1N1B4_87at 91.620 kb on - strand, within Pf1N1B4_87at 91.620 kb on - strand, within Pf1N1B4_87at 91.658 kb on + strand, within Pf1N1B4_87at 91.659 kb on - strand, within Pf1N1B4_87at 91.659 kb on - strand, within Pf1N1B4_87at 91.817 kb on + strand, within Pf1N1B4_87at 91.817 kb on + strand, within Pf1N1B4_87at 91.817 kb on + strand, within Pf1N1B4_87at 91.849 kb on + strand, within Pf1N1B4_87at 91.850 kb on - strand, within Pf1N1B4_87at 91.850 kb on - strand, within Pf1N1B4_87at 92.152 kb on + strandat 92.181 kb on + strandat 92.182 kb on - strandat 92.198 kb on + strandat 92.198 kb on + strandat 92.347 kb on + strandat 92.347 kb on + strandat 92.370 kb on + strandat 92.370 kb on + strandat 92.446 kb on - strandat 92.545 kb on + strand, within Pf1N1B4_88at 92.545 kb on + strand, within Pf1N1B4_88at 92.546 kb on - strand, within Pf1N1B4_88at 92.546 kb on - strand, within Pf1N1B4_88at 92.546 kb on - strandat 92.546 kb on - strand, within Pf1N1B4_88at 92.660 kb on + strand, within Pf1N1B4_88at 92.661 kb on - strand, within Pf1N1B4_88at 92.909 kb on + strandat 92.910 kb on - strandat 92.910 kb on - strandat 92.910 kb on - strandat 92.910 kb on - strandat 92.991 kb on + strandat 92.995 kb on + strandat 92.995 kb on + strandat 92.995 kb on + strandat 92.996 kb on - strandat 93.008 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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88,820 + Pf1N1B4_85 0.37 -1.5
88,820 + Pf1N1B4_85 0.37 +0.5
88,994 + Pf1N1B4_85 0.58 -0.2
88,994 + Pf1N1B4_85 0.58 +0.5
88,995 - Pf1N1B4_85 0.59 -0.0
88,996 + +0.8
89,070 - Pf1N1B4_85 0.68 -1.2
89,070 - Pf1N1B4_85 0.68 +0.5
89,070 - Pf1N1B4_85 0.68 -2.8
89,311 + +0.5
89,311 + -2.0
89,312 - -1.8
89,312 - -0.4
89,312 - -0.8
89,312 - +0.3
89,491 - Pf1N1B4_86 0.27 -2.2
89,536 + Pf1N1B4_86 0.36 -0.2
89,536 + Pf1N1B4_86 0.36 +0.4
89,897 + +0.5
89,897 + +2.1
89,959 + -0.5
89,959 + -2.5
89,959 + +0.0
89,960 - +0.8
89,960 - +0.1
89,963 + +0.4
89,963 + -1.8
89,963 + -0.4
89,964 - -0.2
89,964 - -0.7
89,964 - +1.4
89,964 - +0.8
90,018 - -3.7
90,018 - +0.3
90,060 + Pf1N1B4_87 0.11 +0.8
90,061 - Pf1N1B4_87 0.11 -1.8
90,061 - Pf1N1B4_87 0.11 -0.6
90,068 + Pf1N1B4_87 0.11 +1.0
90,146 + Pf1N1B4_87 0.14 +0.4
90,147 - Pf1N1B4_87 0.15 -2.2
90,158 + Pf1N1B4_87 0.15 +0.2
90,194 + Pf1N1B4_87 0.16 -1.5
90,194 + Pf1N1B4_87 0.16 +0.8
90,195 - Pf1N1B4_87 0.17 -0.0
90,261 - Pf1N1B4_87 0.19 -1.6
90,363 - Pf1N1B4_87 0.24 +0.0
90,363 - Pf1N1B4_87 0.24 -1.3
90,530 + Pf1N1B4_87 0.31 -1.5
90,530 + Pf1N1B4_87 0.31 -0.2
90,659 + Pf1N1B4_87 0.36 -3.2
90,659 + Pf1N1B4_87 0.36 +1.0
90,659 + Pf1N1B4_87 0.36 -1.6
90,660 - Pf1N1B4_87 0.36 -1.8
90,660 - Pf1N1B4_87 0.36 +0.1
90,660 - Pf1N1B4_87 0.36 -1.2
91,026 + Pf1N1B4_87 0.51 -3.0
91,026 + Pf1N1B4_87 0.51 -0.2
91,026 + Pf1N1B4_87 0.51 -0.1
91,027 - Pf1N1B4_87 0.51 +3.5
91,028 + Pf1N1B4_87 0.51 +0.1
91,028 + Pf1N1B4_87 0.51 +0.1
91,028 + Pf1N1B4_87 0.51 +0.8
91,028 + Pf1N1B4_87 0.51 -0.2
91,029 - -0.2
91,029 - Pf1N1B4_87 0.51 +0.1
91,029 - Pf1N1B4_87 0.51 -0.4
91,029 - Pf1N1B4_87 0.51 -0.2
91,029 - Pf1N1B4_87 0.51 -0.5
91,029 - Pf1N1B4_87 0.51 +0.3
91,029 - Pf1N1B4_87 0.51 +2.1
91,029 - Pf1N1B4_87 0.51 +0.5
91,029 - +0.8
91,029 - Pf1N1B4_87 0.51 -0.5
91,166 + Pf1N1B4_87 0.57 -2.8
91,166 + Pf1N1B4_87 0.57 +1.0
91,167 - -0.2
91,364 + Pf1N1B4_87 0.65 -1.8
91,364 + +0.4
91,365 - Pf1N1B4_87 0.66 -1.5
91,365 - Pf1N1B4_87 0.66 -0.4
91,365 - Pf1N1B4_87 0.66 +0.0
91,365 - Pf1N1B4_87 0.66 +1.3
91,565 + Pf1N1B4_87 0.74 -0.2
91,566 - Pf1N1B4_87 0.74 -1.0
91,619 + +0.8
91,619 + Pf1N1B4_87 0.76 +1.0
91,619 + Pf1N1B4_87 0.76 +2.4
91,619 + Pf1N1B4_87 0.76 +0.8
91,619 + Pf1N1B4_87 0.76 -0.8
91,619 + Pf1N1B4_87 0.76 -2.2
91,619 + Pf1N1B4_87 0.76 -1.8
91,619 + Pf1N1B4_87 0.76 -0.5
91,619 + Pf1N1B4_87 0.76 +0.1
91,619 + Pf1N1B4_87 0.76 +0.4
91,619 + Pf1N1B4_87 0.76 -0.2
91,620 - Pf1N1B4_87 0.76 -0.7
91,620 - Pf1N1B4_87 0.76 -0.1
91,620 - Pf1N1B4_87 0.76 -0.2
91,658 + Pf1N1B4_87 0.78 -0.5
91,659 - Pf1N1B4_87 0.78 -0.2
91,659 - Pf1N1B4_87 0.78 -2.8
91,817 + Pf1N1B4_87 0.84 -1.2
91,817 + Pf1N1B4_87 0.84 -0.9
91,817 + Pf1N1B4_87 0.84 -1.8
91,849 + Pf1N1B4_87 0.86 -0.3
91,850 - Pf1N1B4_87 0.86 +0.0
91,850 - Pf1N1B4_87 0.86 -1.8
92,152 + +0.8
92,181 + +0.1
92,182 - +2.1
92,198 + -0.5
92,198 + +0.0
92,347 + -0.4
92,347 + -0.2
92,370 + +2.8
92,370 + -0.6
92,446 - -0.2
92,545 + Pf1N1B4_88 0.13 +1.4
92,545 + Pf1N1B4_88 0.13 -0.2
92,546 - Pf1N1B4_88 0.14 -0.2
92,546 - Pf1N1B4_88 0.14 -2.7
92,546 - -0.2
92,546 - Pf1N1B4_88 0.14 -2.2
92,660 + Pf1N1B4_88 0.39 -0.8
92,661 - Pf1N1B4_88 0.40 -1.2
92,909 + -0.5
92,910 - +2.8
92,910 - -1.2
92,910 - -0.2
92,910 - -2.1
92,991 + -0.2
92,995 + -1.8
92,995 + +2.1
92,995 + +2.1
92,996 - +0.5
93,008 - -0.2

Or see this region's nucleotide sequence