Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_85 and Pf1N1B4_86 are separated by 29 nucleotides Pf1N1B4_86 and Pf1N1B4_87 overlap by 36 nucleotides Pf1N1B4_87 and Pf1N1B4_88 are separated by 298 nucleotides Pf1N1B4_88 and Pf1N1B4_89 are separated by 131 nucleotides
Pf1N1B4_85 - Oxidoreductase, aldo/keto reductase family, at 88,514 to 89,335
_85
Pf1N1B4_86 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 89,365 to 89,835
_86
Pf1N1B4_87 - DNA polymerase II (EC 2.7.7.7), at 89,800 to 92,187
_87
Pf1N1B4_88 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 92,486 to 92,926
_88
Pf1N1B4_89 - Uncharacterized GST-like protein yncG, at 93,058 to 93,678
_89
Position (kb)
89
90
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 88.820 kb on + strand, within Pf1N1B4_85 at 88.820 kb on + strand, within Pf1N1B4_85 at 88.994 kb on + strand, within Pf1N1B4_85 at 88.994 kb on + strand, within Pf1N1B4_85 at 88.995 kb on - strand, within Pf1N1B4_85 at 88.996 kb on + strand at 89.070 kb on - strand, within Pf1N1B4_85 at 89.070 kb on - strand, within Pf1N1B4_85 at 89.070 kb on - strand, within Pf1N1B4_85 at 89.311 kb on + strand at 89.311 kb on + strand at 89.312 kb on - strand at 89.312 kb on - strand at 89.312 kb on - strand at 89.312 kb on - strand at 89.491 kb on - strand, within Pf1N1B4_86 at 89.536 kb on + strand, within Pf1N1B4_86 at 89.536 kb on + strand, within Pf1N1B4_86 at 89.897 kb on + strand at 89.897 kb on + strand at 89.959 kb on + strand at 89.959 kb on + strand at 89.959 kb on + strand at 89.960 kb on - strand at 89.960 kb on - strand at 89.963 kb on + strand at 89.963 kb on + strand at 89.963 kb on + strand at 89.964 kb on - strand at 89.964 kb on - strand at 89.964 kb on - strand at 89.964 kb on - strand at 90.018 kb on - strand at 90.018 kb on - strand at 90.060 kb on + strand, within Pf1N1B4_87 at 90.061 kb on - strand, within Pf1N1B4_87 at 90.061 kb on - strand, within Pf1N1B4_87 at 90.068 kb on + strand, within Pf1N1B4_87 at 90.146 kb on + strand, within Pf1N1B4_87 at 90.147 kb on - strand, within Pf1N1B4_87 at 90.158 kb on + strand, within Pf1N1B4_87 at 90.194 kb on + strand, within Pf1N1B4_87 at 90.194 kb on + strand, within Pf1N1B4_87 at 90.195 kb on - strand, within Pf1N1B4_87 at 90.261 kb on - strand, within Pf1N1B4_87 at 90.363 kb on - strand, within Pf1N1B4_87 at 90.363 kb on - strand, within Pf1N1B4_87 at 90.530 kb on + strand, within Pf1N1B4_87 at 90.530 kb on + strand, within Pf1N1B4_87 at 90.659 kb on + strand, within Pf1N1B4_87 at 90.659 kb on + strand, within Pf1N1B4_87 at 90.659 kb on + strand, within Pf1N1B4_87 at 90.660 kb on - strand, within Pf1N1B4_87 at 90.660 kb on - strand, within Pf1N1B4_87 at 90.660 kb on - strand, within Pf1N1B4_87 at 91.026 kb on + strand, within Pf1N1B4_87 at 91.026 kb on + strand, within Pf1N1B4_87 at 91.026 kb on + strand, within Pf1N1B4_87 at 91.027 kb on - strand, within Pf1N1B4_87 at 91.028 kb on + strand, within Pf1N1B4_87 at 91.028 kb on + strand, within Pf1N1B4_87 at 91.028 kb on + strand, within Pf1N1B4_87 at 91.028 kb on + strand, within Pf1N1B4_87 at 91.029 kb on - strand at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand, within Pf1N1B4_87 at 91.029 kb on - strand at 91.029 kb on - strand, within Pf1N1B4_87 at 91.166 kb on + strand, within Pf1N1B4_87 at 91.166 kb on + strand, within Pf1N1B4_87 at 91.167 kb on - strand at 91.364 kb on + strand, within Pf1N1B4_87 at 91.364 kb on + strand at 91.365 kb on - strand, within Pf1N1B4_87 at 91.365 kb on - strand, within Pf1N1B4_87 at 91.365 kb on - strand, within Pf1N1B4_87 at 91.365 kb on - strand, within Pf1N1B4_87 at 91.565 kb on + strand, within Pf1N1B4_87 at 91.566 kb on - strand, within Pf1N1B4_87 at 91.619 kb on + strand at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.619 kb on + strand, within Pf1N1B4_87 at 91.620 kb on - strand, within Pf1N1B4_87 at 91.620 kb on - strand, within Pf1N1B4_87 at 91.620 kb on - strand, within Pf1N1B4_87 at 91.658 kb on + strand, within Pf1N1B4_87 at 91.659 kb on - strand, within Pf1N1B4_87 at 91.659 kb on - strand, within Pf1N1B4_87 at 91.817 kb on + strand, within Pf1N1B4_87 at 91.817 kb on + strand, within Pf1N1B4_87 at 91.817 kb on + strand, within Pf1N1B4_87 at 91.849 kb on + strand, within Pf1N1B4_87 at 91.850 kb on - strand, within Pf1N1B4_87 at 91.850 kb on - strand, within Pf1N1B4_87 at 92.152 kb on + strand at 92.181 kb on + strand at 92.182 kb on - strand at 92.198 kb on + strand at 92.198 kb on + strand at 92.347 kb on + strand at 92.347 kb on + strand at 92.370 kb on + strand at 92.370 kb on + strand at 92.446 kb on - strand at 92.545 kb on + strand, within Pf1N1B4_88 at 92.545 kb on + strand, within Pf1N1B4_88 at 92.546 kb on - strand, within Pf1N1B4_88 at 92.546 kb on - strand, within Pf1N1B4_88 at 92.546 kb on - strand at 92.546 kb on - strand, within Pf1N1B4_88 at 92.660 kb on + strand, within Pf1N1B4_88 at 92.661 kb on - strand, within Pf1N1B4_88 at 92.909 kb on + strand at 92.910 kb on - strand at 92.910 kb on - strand at 92.910 kb on - strand at 92.910 kb on - strand at 92.991 kb on + strand at 92.995 kb on + strand at 92.995 kb on + strand at 92.995 kb on + strand at 92.996 kb on - strand at 93.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 88,820 + Pf1N1B4_85 0.37 -1.5 88,820 + Pf1N1B4_85 0.37 +0.5 88,994 + Pf1N1B4_85 0.58 -0.2 88,994 + Pf1N1B4_85 0.58 +0.5 88,995 - Pf1N1B4_85 0.59 -0.0 88,996 + +0.8 89,070 - Pf1N1B4_85 0.68 -1.2 89,070 - Pf1N1B4_85 0.68 +0.5 89,070 - Pf1N1B4_85 0.68 -2.8 89,311 + +0.5 89,311 + -2.0 89,312 - -1.8 89,312 - -0.4 89,312 - -0.8 89,312 - +0.3 89,491 - Pf1N1B4_86 0.27 -2.2 89,536 + Pf1N1B4_86 0.36 -0.2 89,536 + Pf1N1B4_86 0.36 +0.4 89,897 + +0.5 89,897 + +2.1 89,959 + -0.5 89,959 + -2.5 89,959 + +0.0 89,960 - +0.8 89,960 - +0.1 89,963 + +0.4 89,963 + -1.8 89,963 + -0.4 89,964 - -0.2 89,964 - -0.7 89,964 - +1.4 89,964 - +0.8 90,018 - -3.7 90,018 - +0.3 90,060 + Pf1N1B4_87 0.11 +0.8 90,061 - Pf1N1B4_87 0.11 -1.8 90,061 - Pf1N1B4_87 0.11 -0.6 90,068 + Pf1N1B4_87 0.11 +1.0 90,146 + Pf1N1B4_87 0.14 +0.4 90,147 - Pf1N1B4_87 0.15 -2.2 90,158 + Pf1N1B4_87 0.15 +0.2 90,194 + Pf1N1B4_87 0.16 -1.5 90,194 + Pf1N1B4_87 0.16 +0.8 90,195 - Pf1N1B4_87 0.17 -0.0 90,261 - Pf1N1B4_87 0.19 -1.6 90,363 - Pf1N1B4_87 0.24 +0.0 90,363 - Pf1N1B4_87 0.24 -1.3 90,530 + Pf1N1B4_87 0.31 -1.5 90,530 + Pf1N1B4_87 0.31 -0.2 90,659 + Pf1N1B4_87 0.36 -3.2 90,659 + Pf1N1B4_87 0.36 +1.0 90,659 + Pf1N1B4_87 0.36 -1.6 90,660 - Pf1N1B4_87 0.36 -1.8 90,660 - Pf1N1B4_87 0.36 +0.1 90,660 - Pf1N1B4_87 0.36 -1.2 91,026 + Pf1N1B4_87 0.51 -3.0 91,026 + Pf1N1B4_87 0.51 -0.2 91,026 + Pf1N1B4_87 0.51 -0.1 91,027 - Pf1N1B4_87 0.51 +3.5 91,028 + Pf1N1B4_87 0.51 +0.1 91,028 + Pf1N1B4_87 0.51 +0.1 91,028 + Pf1N1B4_87 0.51 +0.8 91,028 + Pf1N1B4_87 0.51 -0.2 91,029 - -0.2 91,029 - Pf1N1B4_87 0.51 +0.1 91,029 - Pf1N1B4_87 0.51 -0.4 91,029 - Pf1N1B4_87 0.51 -0.2 91,029 - Pf1N1B4_87 0.51 -0.5 91,029 - Pf1N1B4_87 0.51 +0.3 91,029 - Pf1N1B4_87 0.51 +2.1 91,029 - Pf1N1B4_87 0.51 +0.5 91,029 - +0.8 91,029 - Pf1N1B4_87 0.51 -0.5 91,166 + Pf1N1B4_87 0.57 -2.8 91,166 + Pf1N1B4_87 0.57 +1.0 91,167 - -0.2 91,364 + Pf1N1B4_87 0.65 -1.8 91,364 + +0.4 91,365 - Pf1N1B4_87 0.66 -1.5 91,365 - Pf1N1B4_87 0.66 -0.4 91,365 - Pf1N1B4_87 0.66 +0.0 91,365 - Pf1N1B4_87 0.66 +1.3 91,565 + Pf1N1B4_87 0.74 -0.2 91,566 - Pf1N1B4_87 0.74 -1.0 91,619 + +0.8 91,619 + Pf1N1B4_87 0.76 +1.0 91,619 + Pf1N1B4_87 0.76 +2.4 91,619 + Pf1N1B4_87 0.76 +0.8 91,619 + Pf1N1B4_87 0.76 -0.8 91,619 + Pf1N1B4_87 0.76 -2.2 91,619 + Pf1N1B4_87 0.76 -1.8 91,619 + Pf1N1B4_87 0.76 -0.5 91,619 + Pf1N1B4_87 0.76 +0.1 91,619 + Pf1N1B4_87 0.76 +0.4 91,619 + Pf1N1B4_87 0.76 -0.2 91,620 - Pf1N1B4_87 0.76 -0.7 91,620 - Pf1N1B4_87 0.76 -0.1 91,620 - Pf1N1B4_87 0.76 -0.2 91,658 + Pf1N1B4_87 0.78 -0.5 91,659 - Pf1N1B4_87 0.78 -0.2 91,659 - Pf1N1B4_87 0.78 -2.8 91,817 + Pf1N1B4_87 0.84 -1.2 91,817 + Pf1N1B4_87 0.84 -0.9 91,817 + Pf1N1B4_87 0.84 -1.8 91,849 + Pf1N1B4_87 0.86 -0.3 91,850 - Pf1N1B4_87 0.86 +0.0 91,850 - Pf1N1B4_87 0.86 -1.8 92,152 + +0.8 92,181 + +0.1 92,182 - +2.1 92,198 + -0.5 92,198 + +0.0 92,347 + -0.4 92,347 + -0.2 92,370 + +2.8 92,370 + -0.6 92,446 - -0.2 92,545 + Pf1N1B4_88 0.13 +1.4 92,545 + Pf1N1B4_88 0.13 -0.2 92,546 - Pf1N1B4_88 0.14 -0.2 92,546 - Pf1N1B4_88 0.14 -2.7 92,546 - -0.2 92,546 - Pf1N1B4_88 0.14 -2.2 92,660 + Pf1N1B4_88 0.39 -0.8 92,661 - Pf1N1B4_88 0.40 -1.2 92,909 + -0.5 92,910 - +2.8 92,910 - -1.2 92,910 - -0.2 92,910 - -2.1 92,991 + -0.2 92,995 + -1.8 92,995 + +2.1 92,995 + +2.1 92,996 - +0.5 93,008 - -0.2
Or see this region's nucleotide sequence