Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_768 and Pf1N1B4_769 are separated by 369 nucleotides Pf1N1B4_769 and Pf1N1B4_770 are separated by 324 nucleotides Pf1N1B4_770 and Pf1N1B4_771 overlap by 18 nucleotides
Pf1N1B4_768 - Glycerol-3-phosphate regulon repressor, DeoR family, at 872,576 to 873,331
_768
Pf1N1B4_769 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 873,701 to 875,161
_769
Pf1N1B4_770 - hypothetical protein, at 875,486 to 875,626
_770
Pf1N1B4_771 - ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component <small>(from data)</small>, at 875,609 to 876,523
_771
Position (kb) 873
874
875
876 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 873.329 kb on + strand at 873.398 kb on + strand at 873.398 kb on + strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.399 kb on - strand at 873.401 kb on - strand at 873.401 kb on - strand at 873.474 kb on - strand at 873.474 kb on - strand at 873.474 kb on - strand at 873.474 kb on - strand at 873.474 kb on - strand at 873.474 kb on - strand at 873.595 kb on + strand at 873.597 kb on + strand at 873.635 kb on + strand at 873.635 kb on + strand at 873.635 kb on + strand at 873.635 kb on + strand at 873.636 kb on - strand at 873.636 kb on - strand at 873.636 kb on - strand at 873.644 kb on + strand at 873.644 kb on + strand at 873.665 kb on + strand at 873.665 kb on + strand at 873.665 kb on + strand at 873.665 kb on + strand at 873.666 kb on - strand at 873.831 kb on - strand at 873.977 kb on + strand, within Pf1N1B4_769 at 874.116 kb on + strand, within Pf1N1B4_769 at 874.116 kb on + strand, within Pf1N1B4_769 at 874.370 kb on + strand, within Pf1N1B4_769 at 874.370 kb on + strand, within Pf1N1B4_769 at 874.370 kb on + strand, within Pf1N1B4_769 at 874.475 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.652 kb on + strand, within Pf1N1B4_769 at 874.653 kb on - strand, within Pf1N1B4_769 at 874.766 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.957 kb on + strand, within Pf1N1B4_769 at 874.991 kb on + strand, within Pf1N1B4_769 at 874.991 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.114 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.226 kb on - strand at 875.226 kb on - strand at 875.281 kb on + strand at 875.282 kb on - strand at 875.284 kb on + strand at 875.284 kb on + strand at 875.285 kb on - strand at 875.285 kb on - strand at 875.299 kb on + strand at 875.340 kb on - strand at 875.410 kb on - strand at 875.464 kb on - strand at 875.494 kb on + strand at 875.582 kb on + strand, within Pf1N1B4_770 at 875.582 kb on + strand, within Pf1N1B4_770 at 875.583 kb on - strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.762 kb on + strand, within Pf1N1B4_771 at 875.795 kb on + strand, within Pf1N1B4_771 at 875.871 kb on - strand, within Pf1N1B4_771 at 875.871 kb on - strand, within Pf1N1B4_771 at 876.035 kb on + strand, within Pf1N1B4_771 at 876.036 kb on - strand, within Pf1N1B4_771 at 876.036 kb on - strand at 876.036 kb on - strand, within Pf1N1B4_771
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 873,329 + -0.5 873,398 + -1.3 873,398 + +1.2 873,399 - -2.0 873,399 - -1.8 873,399 - -1.8 873,399 - -0.7 873,399 - +1.6 873,399 - +0.0 873,399 - -0.2 873,399 - +1.6 873,401 - -2.3 873,401 - -0.1 873,474 - -0.8 873,474 - +1.6 873,474 - -0.8 873,474 - -2.2 873,474 - -0.4 873,474 - -0.1 873,595 + -1.5 873,597 + -1.5 873,635 + -3.4 873,635 + -2.5 873,635 + -1.6 873,635 + -1.5 873,636 - -1.0 873,636 - -0.1 873,636 - -1.5 873,644 + -0.4 873,644 + -1.0 873,665 + -2.3 873,665 + -2.0 873,665 + -2.3 873,665 + -1.5 873,666 - -0.8 873,831 - -1.0 873,977 + Pf1N1B4_769 0.19 +0.0 874,116 + Pf1N1B4_769 0.28 -1.0 874,116 + Pf1N1B4_769 0.28 -1.0 874,370 + Pf1N1B4_769 0.46 -2.3 874,370 + Pf1N1B4_769 0.46 -1.4 874,370 + Pf1N1B4_769 0.46 -2.3 874,475 + Pf1N1B4_769 0.53 +0.0 874,589 + Pf1N1B4_769 0.61 -3.3 874,589 + Pf1N1B4_769 0.61 -1.0 874,589 + Pf1N1B4_769 0.61 -3.5 874,589 + Pf1N1B4_769 0.61 -0.4 874,652 + Pf1N1B4_769 0.65 -2.3 874,653 - Pf1N1B4_769 0.65 -1.5 874,766 + Pf1N1B4_769 0.73 +0.0 874,913 + Pf1N1B4_769 0.83 -1.1 874,913 + Pf1N1B4_769 0.83 -1.5 874,913 + Pf1N1B4_769 0.83 -1.9 874,913 + Pf1N1B4_769 0.83 -2.3 874,957 + Pf1N1B4_769 0.86 -2.5 874,991 + Pf1N1B4_769 0.88 -1.5 874,991 + Pf1N1B4_769 0.88 -2.8 875,009 + Pf1N1B4_769 0.90 -1.4 875,009 + Pf1N1B4_769 0.90 -0.5 875,009 + Pf1N1B4_769 0.90 -1.7 875,114 + +0.0 875,225 + -2.0 875,225 + -2.0 875,225 + +0.9 875,225 + +1.0 875,226 - -2.5 875,226 - -0.7 875,281 + +0.0 875,282 - +0.0 875,284 + +0.5 875,284 + +0.1 875,285 - -2.3 875,285 - +0.9 875,299 + +1.2 875,340 - +0.0 875,410 - -0.2 875,464 - -0.4 875,494 + +1.2 875,582 + Pf1N1B4_770 0.68 -2.3 875,582 + Pf1N1B4_770 0.68 +1.6 875,583 - Pf1N1B4_770 0.69 -2.0 875,584 + Pf1N1B4_770 0.70 -0.3 875,584 + Pf1N1B4_770 0.70 +1.3 875,584 + Pf1N1B4_770 0.70 -0.4 875,584 + Pf1N1B4_770 0.70 +1.5 875,762 + Pf1N1B4_771 0.17 +0.0 875,795 + Pf1N1B4_771 0.20 +0.0 875,871 - Pf1N1B4_771 0.29 +0.0 875,871 - Pf1N1B4_771 0.29 +0.9 876,035 + Pf1N1B4_771 0.47 -1.0 876,036 - Pf1N1B4_771 0.47 +1.6 876,036 - +0.0 876,036 - Pf1N1B4_771 0.47 +0.5
Or see this region's nucleotide sequence