Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_690 and Pf1N1B4_691 are separated by 129 nucleotides Pf1N1B4_691 and Pf1N1B4_692 are separated by 15 nucleotides Pf1N1B4_692 and Pf1N1B4_693 overlap by 4 nucleotides
Pf1N1B4_690 - Transcriptional regulator, AraC family, at 788,136 to 789,065
_690
Pf1N1B4_691 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 789,195 to 790,250
_691
Pf1N1B4_692 - ABC spermidine/putrescine transporter, inner membrane subunit, at 790,266 to 791,114
_692
Pf1N1B4_693 - ABC spermidine/putrescine transporter, inner membrane subunit, at 791,111 to 791,917
_693
Position (kb)
789
790
791 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 788.220 kb on - strand at 788.241 kb on + strand, within Pf1N1B4_690 at 788.241 kb on + strand, within Pf1N1B4_690 at 788.242 kb on - strand at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.242 kb on - strand, within Pf1N1B4_690 at 788.370 kb on + strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.371 kb on - strand, within Pf1N1B4_690 at 788.379 kb on + strand, within Pf1N1B4_690 at 788.380 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.395 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.438 kb on - strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.562 kb on + strand, within Pf1N1B4_690 at 788.563 kb on - strand, within Pf1N1B4_690 at 788.781 kb on + strand, within Pf1N1B4_690 at 788.782 kb on - strand, within Pf1N1B4_690 at 788.782 kb on - strand, within Pf1N1B4_690 at 788.782 kb on - strand, within Pf1N1B4_690 at 788.886 kb on + strand, within Pf1N1B4_690 at 789.003 kb on + strand at 789.003 kb on + strand at 789.130 kb on + strand at 789.130 kb on + strand at 789.131 kb on - strand at 789.282 kb on - strand at 789.328 kb on - strand, within Pf1N1B4_691 at 789.328 kb on - strand, within Pf1N1B4_691 at 789.552 kb on - strand, within Pf1N1B4_691 at 789.552 kb on - strand, within Pf1N1B4_691 at 789.586 kb on + strand, within Pf1N1B4_691 at 789.587 kb on - strand, within Pf1N1B4_691 at 790.025 kb on + strand, within Pf1N1B4_691 at 790.044 kb on - strand, within Pf1N1B4_691 at 790.044 kb on - strand, within Pf1N1B4_691 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.390 kb on + strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.391 kb on - strand, within Pf1N1B4_692 at 790.427 kb on - strand, within Pf1N1B4_692 at 790.427 kb on - strand, within Pf1N1B4_692 at 790.435 kb on + strand, within Pf1N1B4_692 at 790.436 kb on - strand, within Pf1N1B4_692 at 790.436 kb on - strand, within Pf1N1B4_692 at 790.798 kb on + strand, within Pf1N1B4_692 at 790.798 kb on + strand, within Pf1N1B4_692 at 790.799 kb on - strand at 790.799 kb on - strand, within Pf1N1B4_692 at 790.799 kb on - strand, within Pf1N1B4_692 at 790.799 kb on - strand, within Pf1N1B4_692 at 790.933 kb on + strand at 790.934 kb on - strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.948 kb on + strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 790.949 kb on - strand, within Pf1N1B4_692 at 791.154 kb on + strand at 791.154 kb on + strand at 791.155 kb on - strand at 791.155 kb on - strand at 791.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 788,220 - +0.6 788,241 + Pf1N1B4_690 0.11 +0.5 788,241 + Pf1N1B4_690 0.11 -1.0 788,242 - -1.0 788,242 - Pf1N1B4_690 0.11 -1.0 788,242 - Pf1N1B4_690 0.11 -2.4 788,242 - Pf1N1B4_690 0.11 +0.3 788,242 - Pf1N1B4_690 0.11 -0.1 788,370 + Pf1N1B4_690 0.25 +2.6 788,371 - Pf1N1B4_690 0.25 +2.5 788,371 - Pf1N1B4_690 0.25 +0.6 788,371 - Pf1N1B4_690 0.25 +0.1 788,371 - Pf1N1B4_690 0.25 -2.3 788,371 - Pf1N1B4_690 0.25 +0.8 788,371 - Pf1N1B4_690 0.25 -0.2 788,379 + Pf1N1B4_690 0.26 -0.5 788,380 - Pf1N1B4_690 0.26 +2.0 788,395 - Pf1N1B4_690 0.28 +0.7 788,395 - Pf1N1B4_690 0.28 -0.4 788,395 - Pf1N1B4_690 0.28 +1.8 788,438 - Pf1N1B4_690 0.32 -2.3 788,438 - Pf1N1B4_690 0.32 +0.5 788,438 - Pf1N1B4_690 0.32 +0.4 788,438 - Pf1N1B4_690 0.32 -1.5 788,562 + Pf1N1B4_690 0.46 -1.0 788,562 + Pf1N1B4_690 0.46 +0.0 788,562 + Pf1N1B4_690 0.46 -0.5 788,562 + Pf1N1B4_690 0.46 -1.0 788,563 - Pf1N1B4_690 0.46 +1.3 788,781 + Pf1N1B4_690 0.69 +0.6 788,782 - Pf1N1B4_690 0.69 -1.5 788,782 - Pf1N1B4_690 0.69 +1.9 788,782 - Pf1N1B4_690 0.69 +0.5 788,886 + Pf1N1B4_690 0.81 -2.0 789,003 + +0.6 789,003 + +1.6 789,130 + -2.3 789,130 + +2.8 789,131 - +2.0 789,282 - +1.0 789,328 - Pf1N1B4_691 0.13 -1.0 789,328 - Pf1N1B4_691 0.13 +0.4 789,552 - Pf1N1B4_691 0.34 +2.0 789,552 - Pf1N1B4_691 0.34 -2.0 789,586 + Pf1N1B4_691 0.37 -1.8 789,587 - Pf1N1B4_691 0.37 -2.8 790,025 + Pf1N1B4_691 0.79 -1.2 790,044 - Pf1N1B4_691 0.80 +1.0 790,044 - Pf1N1B4_691 0.80 -1.0 790,390 + Pf1N1B4_692 0.15 +0.0 790,390 + Pf1N1B4_692 0.15 +0.0 790,390 + Pf1N1B4_692 0.15 +0.6 790,390 + Pf1N1B4_692 0.15 +0.3 790,390 + Pf1N1B4_692 0.15 -1.5 790,390 + Pf1N1B4_692 0.15 +2.6 790,390 + Pf1N1B4_692 0.15 -1.0 790,391 - Pf1N1B4_692 0.15 -2.1 790,391 - Pf1N1B4_692 0.15 -0.4 790,391 - Pf1N1B4_692 0.15 +1.0 790,391 - Pf1N1B4_692 0.15 +2.0 790,427 - Pf1N1B4_692 0.19 +0.4 790,427 - Pf1N1B4_692 0.19 +2.5 790,435 + Pf1N1B4_692 0.20 +2.6 790,436 - Pf1N1B4_692 0.20 -2.1 790,436 - Pf1N1B4_692 0.20 -2.3 790,798 + Pf1N1B4_692 0.63 +0.0 790,798 + Pf1N1B4_692 0.63 -0.4 790,799 - +1.6 790,799 - Pf1N1B4_692 0.63 -1.0 790,799 - Pf1N1B4_692 0.63 -2.0 790,799 - Pf1N1B4_692 0.63 +1.4 790,933 + -0.5 790,934 - Pf1N1B4_692 0.79 +0.2 790,948 + Pf1N1B4_692 0.80 -2.5 790,948 + Pf1N1B4_692 0.80 +0.0 790,948 + Pf1N1B4_692 0.80 -1.0 790,949 - Pf1N1B4_692 0.80 -1.0 790,949 - Pf1N1B4_692 0.80 +0.0 790,949 - Pf1N1B4_692 0.80 -1.5 791,154 + -1.0 791,154 + -0.8 791,155 - +1.8 791,155 - +0.0 791,155 - -1.2
Or see this region's nucleotide sequence