Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_665 and Pf1N1B4_666 are separated by 37 nucleotides Pf1N1B4_666 and Pf1N1B4_667 are separated by 170 nucleotides Pf1N1B4_667 and Pf1N1B4_668 are separated by 98 nucleotides Pf1N1B4_668 and Pf1N1B4_669 are separated by 169 nucleotides Pf1N1B4_669 and Pf1N1B4_670 overlap by 4 nucleotides
Pf1N1B4_665 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 762,094 to 762,942
_665
Pf1N1B4_666 - hypothetical protein, at 762,980 to 763,099
_666
Pf1N1B4_667 - transcriptional regulator MvaT, P16 subunit, at 763,270 to 763,647
_667
Pf1N1B4_668 - Exodeoxyribonuclease I (EC 3.1.11.1), at 763,746 to 765,176
_668
Pf1N1B4_669 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 765,346 to 766,071
_669
Pf1N1B4_670 - FIG01248689: hypothetical protein, at 766,068 to 767,045
_670
Position (kb)
763
764
765
766 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 763.017 kb on + strand at 763.115 kb on + strand at 763.115 kb on + strand at 763.131 kb on + strand at 763.131 kb on + strand at 763.166 kb on + strand at 763.175 kb on + strand at 763.257 kb on + strand at 763.308 kb on + strand, within Pf1N1B4_667 at 763.736 kb on - strand at 763.821 kb on - strand at 763.821 kb on - strand at 763.897 kb on - strand, within Pf1N1B4_668 at 763.897 kb on - strand, within Pf1N1B4_668 at 763.897 kb on - strand at 763.984 kb on - strand, within Pf1N1B4_668 at 764.029 kb on - strand, within Pf1N1B4_668 at 764.101 kb on - strand at 764.162 kb on + strand, within Pf1N1B4_668 at 764.247 kb on + strand at 764.248 kb on - strand, within Pf1N1B4_668 at 764.292 kb on + strand, within Pf1N1B4_668 at 764.293 kb on - strand at 764.293 kb on - strand, within Pf1N1B4_668 at 764.293 kb on - strand, within Pf1N1B4_668 at 764.479 kb on + strand, within Pf1N1B4_668 at 764.479 kb on + strand, within Pf1N1B4_668 at 764.479 kb on + strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.511 kb on - strand, within Pf1N1B4_668 at 764.585 kb on - strand, within Pf1N1B4_668 at 764.585 kb on - strand, within Pf1N1B4_668 at 764.585 kb on - strand, within Pf1N1B4_668 at 764.586 kb on + strand, within Pf1N1B4_668 at 764.586 kb on + strand, within Pf1N1B4_668 at 764.586 kb on + strand at 764.587 kb on - strand, within Pf1N1B4_668 at 764.587 kb on - strand, within Pf1N1B4_668 at 764.587 kb on - strand, within Pf1N1B4_668 at 764.628 kb on - strand, within Pf1N1B4_668 at 764.765 kb on - strand, within Pf1N1B4_668 at 764.765 kb on - strand, within Pf1N1B4_668 at 764.827 kb on + strand, within Pf1N1B4_668 at 764.991 kb on - strand at 765.049 kb on + strand at 765.070 kb on - strand at 765.151 kb on + strand at 765.152 kb on - strand at 765.158 kb on - strand at 765.209 kb on + strand at 765.210 kb on - strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.311 kb on - strand at 765.598 kb on + strand at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.751 kb on + strand, within Pf1N1B4_669 at 765.987 kb on - strand, within Pf1N1B4_669
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 763,017 + +0.0 763,115 + +0.0 763,115 + +0.3 763,131 + -1.0 763,131 + -0.5 763,166 + -3.8 763,175 + +0.0 763,257 + +1.8 763,308 + Pf1N1B4_667 0.10 -1.5 763,736 - +2.6 763,821 - -1.0 763,821 - +1.0 763,897 - Pf1N1B4_668 0.11 +0.6 763,897 - Pf1N1B4_668 0.11 +0.0 763,897 - +1.0 763,984 - Pf1N1B4_668 0.17 -0.4 764,029 - Pf1N1B4_668 0.20 -0.5 764,101 - +0.0 764,162 + Pf1N1B4_668 0.29 -1.5 764,247 + -1.5 764,248 - Pf1N1B4_668 0.35 +0.0 764,292 + Pf1N1B4_668 0.38 -1.8 764,293 - -1.0 764,293 - Pf1N1B4_668 0.38 -2.0 764,293 - Pf1N1B4_668 0.38 +0.9 764,479 + Pf1N1B4_668 0.51 -2.0 764,479 + Pf1N1B4_668 0.51 +0.0 764,479 + Pf1N1B4_668 0.51 -1.3 764,480 - Pf1N1B4_668 0.51 -0.4 764,480 - Pf1N1B4_668 0.51 -1.4 764,480 - Pf1N1B4_668 0.51 -1.0 764,480 - Pf1N1B4_668 0.51 -3.1 764,480 - Pf1N1B4_668 0.51 -0.3 764,480 - Pf1N1B4_668 0.51 +1.0 764,480 - Pf1N1B4_668 0.51 -1.0 764,511 - Pf1N1B4_668 0.53 -1.0 764,585 - Pf1N1B4_668 0.59 -2.3 764,585 - Pf1N1B4_668 0.59 -3.1 764,585 - Pf1N1B4_668 0.59 -1.2 764,586 + Pf1N1B4_668 0.59 -1.0 764,586 + Pf1N1B4_668 0.59 +0.6 764,586 + -1.0 764,587 - Pf1N1B4_668 0.59 -2.0 764,587 - Pf1N1B4_668 0.59 -1.0 764,587 - Pf1N1B4_668 0.59 -1.0 764,628 - Pf1N1B4_668 0.62 -2.0 764,765 - Pf1N1B4_668 0.71 -0.7 764,765 - Pf1N1B4_668 0.71 -0.3 764,827 + Pf1N1B4_668 0.76 -1.0 764,991 - -1.0 765,049 + -1.5 765,070 - -1.3 765,151 + -2.0 765,152 - -0.3 765,158 - -1.8 765,209 + +0.9 765,210 - -0.6 765,219 + +1.2 765,219 + -1.5 765,219 + -3.0 765,219 + -0.8 765,219 + +0.3 765,219 + +1.0 765,219 + -1.0 765,219 + -1.5 765,220 - +0.3 765,220 - +0.4 765,220 - -0.2 765,220 - +1.6 765,220 - -1.0 765,276 + -2.1 765,276 + +0.6 765,276 + +0.4 765,276 + +1.6 765,276 + -0.1 765,276 + -2.3 765,277 - -0.2 765,277 - +1.5 765,277 - +1.6 765,277 - +0.6 765,277 - -2.5 765,277 - +0.9 765,280 + -0.5 765,280 + +0.4 765,280 + -0.4 765,280 + +0.0 765,280 + +0.6 765,280 + +1.8 765,280 + +1.6 765,280 + +0.0 765,280 + +1.0 765,280 + +0.0 765,280 + +1.4 765,280 + -2.3 765,280 + -2.0 765,280 + -2.3 765,280 + +0.0 765,280 + -0.4 765,280 + +0.0 765,280 + +1.0 765,280 + +0.0 765,281 - +1.0 765,281 - +0.3 765,281 - +2.0 765,281 - +1.6 765,281 - +0.3 765,281 - +0.3 765,281 - -0.2 765,281 - -0.2 765,281 - +0.6 765,281 - +2.6 765,281 - +0.0 765,281 - +1.4 765,281 - +1.1 765,281 - +0.0 765,281 - -0.5 765,281 - +0.3 765,281 - +1.1 765,281 - -1.5 765,281 - -2.0 765,281 - +2.3 765,281 - -1.5 765,284 + +0.0 765,284 + -0.5 765,284 + -0.2 765,284 + +1.6 765,284 + +0.7 765,284 + -1.3 765,284 + -2.5 765,284 + -1.0 765,284 + +1.0 765,284 + +0.0 765,284 + -1.0 765,284 + +0.6 765,284 + +1.4 765,284 + +2.0 765,284 + +0.5 765,284 + +1.5 765,284 + +0.4 765,285 - -0.0 765,285 - +3.0 765,285 - -0.2 765,285 - -0.8 765,285 - -1.0 765,285 - +0.2 765,285 - +1.0 765,285 - +0.4 765,285 - +0.0 765,285 - -0.7 765,285 - +1.4 765,285 - +1.2 765,285 - -1.0 765,285 - +0.8 765,285 - +1.0 765,285 - +1.0 765,311 - +1.6 765,598 + -2.3 765,598 + Pf1N1B4_669 0.35 +1.5 765,598 + Pf1N1B4_669 0.35 +0.0 765,598 + Pf1N1B4_669 0.35 -0.4 765,598 + Pf1N1B4_669 0.35 +0.0 765,598 + Pf1N1B4_669 0.35 -0.5 765,599 - Pf1N1B4_669 0.35 +1.0 765,599 - Pf1N1B4_669 0.35 -0.6 765,599 - Pf1N1B4_669 0.35 -0.2 765,599 - Pf1N1B4_669 0.35 +0.0 765,599 - Pf1N1B4_669 0.35 -0.5 765,751 + Pf1N1B4_669 0.56 -0.4 765,987 - Pf1N1B4_669 0.88 -1.5
Or see this region's nucleotide sequence