Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_652

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_651 and Pf1N1B4_652 are separated by 45 nucleotidesPf1N1B4_652 and Pf1N1B4_653 are separated by 2 nucleotidesPf1N1B4_653 and Pf1N1B4_654 are separated by 134 nucleotides Pf1N1B4_651 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 744,679 to 745,596 _651 Pf1N1B4_652 - Sensory box histidine kinase, at 745,642 to 747,678 _652 Pf1N1B4_653 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 747,681 to 748,262 _653 Pf1N1B4_654 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 748,397 to 749,341 _654 Position (kb) 745 746 747 748Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 744.696 kb on + strandat 744.696 kb on + strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.697 kb on - strandat 744.791 kb on + strand, within Pf1N1B4_651at 744.792 kb on - strand, within Pf1N1B4_651at 744.792 kb on - strand, within Pf1N1B4_651at 744.792 kb on - strandat 744.850 kb on + strand, within Pf1N1B4_651at 744.850 kb on + strand, within Pf1N1B4_651at 744.903 kb on - strand, within Pf1N1B4_651at 744.903 kb on - strand, within Pf1N1B4_651at 744.919 kb on + strand, within Pf1N1B4_651at 744.919 kb on + strand, within Pf1N1B4_651at 745.114 kb on + strand, within Pf1N1B4_651at 745.115 kb on - strand, within Pf1N1B4_651at 745.182 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.438 kb on + strand, within Pf1N1B4_651at 745.439 kb on - strand, within Pf1N1B4_651at 745.439 kb on - strand, within Pf1N1B4_651at 745.477 kb on + strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.478 kb on - strand, within Pf1N1B4_651at 745.490 kb on - strand, within Pf1N1B4_651at 745.629 kb on - strandat 745.747 kb on - strandat 745.759 kb on + strandat 745.762 kb on + strandat 745.762 kb on + strandat 745.762 kb on + strandat 745.762 kb on + strandat 745.762 kb on + strandat 745.762 kb on + strandat 745.762 kb on + strandat 745.763 kb on - strandat 745.763 kb on - strandat 745.894 kb on + strand, within Pf1N1B4_652at 745.894 kb on + strandat 745.894 kb on + strand, within Pf1N1B4_652at 745.894 kb on + strand, within Pf1N1B4_652at 745.894 kb on + strand, within Pf1N1B4_652at 745.894 kb on + strand, within Pf1N1B4_652at 745.894 kb on + strand, within Pf1N1B4_652at 745.895 kb on - strand, within Pf1N1B4_652at 745.895 kb on - strand, within Pf1N1B4_652at 745.895 kb on - strand, within Pf1N1B4_652at 745.895 kb on - strand, within Pf1N1B4_652at 745.895 kb on - strand, within Pf1N1B4_652at 745.895 kb on - strand, within Pf1N1B4_652at 745.896 kb on + strand, within Pf1N1B4_652at 745.896 kb on + strand, within Pf1N1B4_652at 745.896 kb on + strand, within Pf1N1B4_652at 745.896 kb on + strand, within Pf1N1B4_652at 745.896 kb on + strand, within Pf1N1B4_652at 745.896 kb on + strandat 745.896 kb on + strand, within Pf1N1B4_652at 745.896 kb on + strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strandat 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strandat 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.897 kb on - strand, within Pf1N1B4_652at 745.921 kb on + strand, within Pf1N1B4_652at 745.921 kb on + strand, within Pf1N1B4_652at 745.921 kb on + strand, within Pf1N1B4_652at 745.921 kb on + strand, within Pf1N1B4_652at 745.922 kb on - strandat 745.922 kb on - strand, within Pf1N1B4_652at 745.922 kb on - strand, within Pf1N1B4_652at 745.976 kb on + strand, within Pf1N1B4_652at 746.113 kb on + strand, within Pf1N1B4_652at 746.114 kb on - strand, within Pf1N1B4_652at 746.114 kb on - strand, within Pf1N1B4_652at 746.114 kb on - strand, within Pf1N1B4_652at 746.114 kb on - strand, within Pf1N1B4_652at 746.166 kb on - strand, within Pf1N1B4_652at 746.260 kb on + strand, within Pf1N1B4_652at 746.260 kb on + strand, within Pf1N1B4_652at 746.261 kb on - strand, within Pf1N1B4_652at 746.261 kb on - strand, within Pf1N1B4_652at 746.261 kb on - strand, within Pf1N1B4_652at 746.504 kb on - strand, within Pf1N1B4_652at 746.504 kb on - strand, within Pf1N1B4_652at 746.563 kb on + strand, within Pf1N1B4_652at 746.563 kb on + strand, within Pf1N1B4_652at 746.563 kb on + strand, within Pf1N1B4_652at 746.563 kb on + strand, within Pf1N1B4_652at 746.563 kb on + strand, within Pf1N1B4_652at 746.564 kb on - strand, within Pf1N1B4_652at 746.564 kb on - strand, within Pf1N1B4_652at 746.564 kb on - strand, within Pf1N1B4_652at 746.564 kb on - strand, within Pf1N1B4_652at 746.567 kb on + strand, within Pf1N1B4_652at 746.756 kb on - strand, within Pf1N1B4_652at 746.916 kb on + strand, within Pf1N1B4_652at 746.916 kb on + strand, within Pf1N1B4_652at 746.916 kb on + strand, within Pf1N1B4_652at 746.945 kb on + strand, within Pf1N1B4_652at 746.945 kb on + strand, within Pf1N1B4_652at 746.945 kb on + strand, within Pf1N1B4_652at 746.945 kb on + strand, within Pf1N1B4_652at 746.946 kb on - strand, within Pf1N1B4_652at 747.037 kb on + strandat 747.038 kb on - strand, within Pf1N1B4_652at 747.361 kb on - strand, within Pf1N1B4_652at 747.396 kb on + strand, within Pf1N1B4_652at 747.396 kb on + strand, within Pf1N1B4_652at 747.474 kb on + strand, within Pf1N1B4_652at 747.474 kb on + strand, within Pf1N1B4_652at 747.474 kb on + strand, within Pf1N1B4_652at 747.474 kb on + strand, within Pf1N1B4_652at 747.474 kb on + strand, within Pf1N1B4_652at 747.474 kb on + strand, within Pf1N1B4_652at 747.475 kb on - strandat 747.475 kb on - strandat 747.541 kb on + strandat 747.541 kb on + strandat 747.676 kb on + strandat 747.705 kb on + strandat 747.706 kb on - strandat 747.706 kb on - strandat 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.854 kb on + strand, within Pf1N1B4_653at 747.855 kb on - strand, within Pf1N1B4_653at 747.855 kb on - strand, within Pf1N1B4_653at 747.855 kb on - strand, within Pf1N1B4_653at 747.855 kb on - strand, within Pf1N1B4_653at 747.858 kb on + strand, within Pf1N1B4_653at 747.858 kb on + strand, within Pf1N1B4_653at 747.858 kb on + strand, within Pf1N1B4_653at 747.858 kb on + strand, within Pf1N1B4_653at 747.945 kb on + strand, within Pf1N1B4_653at 747.946 kb on - strand, within Pf1N1B4_653at 747.946 kb on - strand, within Pf1N1B4_653at 748.072 kb on + strand, within Pf1N1B4_653at 748.072 kb on + strand, within Pf1N1B4_653at 748.072 kb on + strand, within Pf1N1B4_653at 748.072 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.155 kb on + strand, within Pf1N1B4_653at 748.156 kb on - strand, within Pf1N1B4_653at 748.156 kb on - strand, within Pf1N1B4_653at 748.156 kb on - strand, within Pf1N1B4_653at 748.156 kb on - strand, within Pf1N1B4_653at 748.157 kb on + strand, within Pf1N1B4_653at 748.157 kb on + strand, within Pf1N1B4_653at 748.157 kb on + strand, within Pf1N1B4_653at 748.158 kb on - strand, within Pf1N1B4_653at 748.158 kb on - strand, within Pf1N1B4_653at 748.260 kb on + strandat 748.320 kb on + strandat 748.508 kb on - strand, within Pf1N1B4_654at 748.525 kb on + strand, within Pf1N1B4_654at 748.525 kb on + strand, within Pf1N1B4_654at 748.525 kb on + strand, within Pf1N1B4_654at 748.526 kb on - strand, within Pf1N1B4_654at 748.526 kb on - strand, within Pf1N1B4_654at 748.526 kb on - strand, within Pf1N1B4_654at 748.526 kb on - strand, within Pf1N1B4_654

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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744,696 + +1.0
744,696 + +1.0
744,697 - +2.5
744,697 - +0.0
744,697 - +0.3
744,697 - +2.0
744,697 - +1.4
744,697 - +0.2
744,697 - -1.6
744,791 + Pf1N1B4_651 0.12 -0.2
744,792 - Pf1N1B4_651 0.12 -0.2
744,792 - Pf1N1B4_651 0.12 +0.8
744,792 - +0.0
744,850 + Pf1N1B4_651 0.19 +0.5
744,850 + Pf1N1B4_651 0.19 +0.0
744,903 - Pf1N1B4_651 0.24 +1.5
744,903 - Pf1N1B4_651 0.24 -0.3
744,919 + Pf1N1B4_651 0.26 -1.8
744,919 + Pf1N1B4_651 0.26 +0.7
745,114 + Pf1N1B4_651 0.47 -0.5
745,115 - Pf1N1B4_651 0.47 -2.0
745,182 + Pf1N1B4_651 0.55 +0.4
745,438 + Pf1N1B4_651 0.83 -1.0
745,438 + Pf1N1B4_651 0.83 +0.9
745,438 + Pf1N1B4_651 0.83 +0.2
745,438 + Pf1N1B4_651 0.83 -0.7
745,439 - Pf1N1B4_651 0.83 +0.0
745,439 - Pf1N1B4_651 0.83 -2.6
745,477 + Pf1N1B4_651 0.87 +0.4
745,478 - Pf1N1B4_651 0.87 +0.6
745,478 - Pf1N1B4_651 0.87 +0.3
745,478 - Pf1N1B4_651 0.87 -1.8
745,478 - Pf1N1B4_651 0.87 -0.7
745,490 - Pf1N1B4_651 0.88 -0.1
745,629 - -0.2
745,747 - -0.4
745,759 + -0.4
745,762 + +1.0
745,762 + +1.6
745,762 + -2.0
745,762 + -2.3
745,762 + -1.0
745,762 + -1.3
745,762 + -0.8
745,763 - -2.1
745,763 - -1.0
745,894 + Pf1N1B4_652 0.12 -2.8
745,894 + -1.0
745,894 + Pf1N1B4_652 0.12 -3.0
745,894 + Pf1N1B4_652 0.12 -3.1
745,894 + Pf1N1B4_652 0.12 -2.6
745,894 + Pf1N1B4_652 0.12 -1.6
745,894 + Pf1N1B4_652 0.12 +0.0
745,895 - Pf1N1B4_652 0.12 -2.0
745,895 - Pf1N1B4_652 0.12 -1.1
745,895 - Pf1N1B4_652 0.12 -2.0
745,895 - Pf1N1B4_652 0.12 -1.6
745,895 - Pf1N1B4_652 0.12 +1.8
745,895 - Pf1N1B4_652 0.12 +0.0
745,896 + Pf1N1B4_652 0.12 +0.0
745,896 + Pf1N1B4_652 0.12 +0.6
745,896 + Pf1N1B4_652 0.12 -1.8
745,896 + Pf1N1B4_652 0.12 -0.2
745,896 + Pf1N1B4_652 0.12 -2.8
745,896 + -0.7
745,896 + Pf1N1B4_652 0.12 -0.7
745,896 + Pf1N1B4_652 0.12 -1.0
745,897 - Pf1N1B4_652 0.13 +1.4
745,897 - Pf1N1B4_652 0.13 -1.3
745,897 - Pf1N1B4_652 0.13 -2.0
745,897 - Pf1N1B4_652 0.13 -0.3
745,897 - -1.2
745,897 - Pf1N1B4_652 0.13 -2.6
745,897 - Pf1N1B4_652 0.13 -3.0
745,897 - Pf1N1B4_652 0.13 -2.2
745,897 - Pf1N1B4_652 0.13 -2.3
745,897 - +0.0
745,897 - Pf1N1B4_652 0.13 -2.1
745,897 - Pf1N1B4_652 0.13 +0.0
745,897 - Pf1N1B4_652 0.13 -0.3
745,921 + Pf1N1B4_652 0.14 -1.6
745,921 + Pf1N1B4_652 0.14 -1.0
745,921 + Pf1N1B4_652 0.14 +0.0
745,921 + Pf1N1B4_652 0.14 -2.6
745,922 - +0.0
745,922 - Pf1N1B4_652 0.14 -1.8
745,922 - Pf1N1B4_652 0.14 -1.6
745,976 + Pf1N1B4_652 0.16 +0.0
746,113 + Pf1N1B4_652 0.23 -2.6
746,114 - Pf1N1B4_652 0.23 -0.4
746,114 - Pf1N1B4_652 0.23 -1.8
746,114 - Pf1N1B4_652 0.23 +0.0
746,114 - Pf1N1B4_652 0.23 -1.3
746,166 - Pf1N1B4_652 0.26 -2.0
746,260 + Pf1N1B4_652 0.30 +0.0
746,260 + Pf1N1B4_652 0.30 +2.4
746,261 - Pf1N1B4_652 0.30 +1.8
746,261 - Pf1N1B4_652 0.30 -2.0
746,261 - Pf1N1B4_652 0.30 -0.6
746,504 - Pf1N1B4_652 0.42 +1.0
746,504 - Pf1N1B4_652 0.42 -2.0
746,563 + Pf1N1B4_652 0.45 -0.4
746,563 + Pf1N1B4_652 0.45 +0.7
746,563 + Pf1N1B4_652 0.45 -2.3
746,563 + Pf1N1B4_652 0.45 -0.7
746,563 + Pf1N1B4_652 0.45 +1.4
746,564 - Pf1N1B4_652 0.45 -3.0
746,564 - Pf1N1B4_652 0.45 -2.8
746,564 - Pf1N1B4_652 0.45 -1.2
746,564 - Pf1N1B4_652 0.45 -1.0
746,567 + Pf1N1B4_652 0.45 +0.6
746,756 - Pf1N1B4_652 0.55 -3.0
746,916 + Pf1N1B4_652 0.63 -1.7
746,916 + Pf1N1B4_652 0.63 -1.8
746,916 + Pf1N1B4_652 0.63 +0.0
746,945 + Pf1N1B4_652 0.64 +0.0
746,945 + Pf1N1B4_652 0.64 -1.1
746,945 + Pf1N1B4_652 0.64 +0.0
746,945 + Pf1N1B4_652 0.64 +0.8
746,946 - Pf1N1B4_652 0.64 -1.0
747,037 + -0.7
747,038 - Pf1N1B4_652 0.69 -3.6
747,361 - Pf1N1B4_652 0.84 -1.3
747,396 + Pf1N1B4_652 0.86 -3.0
747,396 + Pf1N1B4_652 0.86 -1.0
747,474 + Pf1N1B4_652 0.90 -0.3
747,474 + Pf1N1B4_652 0.90 -1.3
747,474 + Pf1N1B4_652 0.90 -1.3
747,474 + Pf1N1B4_652 0.90 -1.6
747,474 + Pf1N1B4_652 0.90 +0.0
747,474 + Pf1N1B4_652 0.90 -0.6
747,475 - -0.2
747,475 - -2.3
747,541 + -2.0
747,541 + -0.6
747,676 + -0.6
747,705 + +0.7
747,706 - -1.0
747,706 - +0.0
747,854 + Pf1N1B4_653 0.30 -0.5
747,854 + Pf1N1B4_653 0.30 -1.8
747,854 + Pf1N1B4_653 0.30 +0.1
747,854 + Pf1N1B4_653 0.30 -1.0
747,854 + Pf1N1B4_653 0.30 +1.0
747,854 + Pf1N1B4_653 0.30 +0.0
747,854 + Pf1N1B4_653 0.30 -0.5
747,854 + Pf1N1B4_653 0.30 +1.4
747,854 + Pf1N1B4_653 0.30 -1.0
747,855 - Pf1N1B4_653 0.30 -1.6
747,855 - Pf1N1B4_653 0.30 -0.4
747,855 - Pf1N1B4_653 0.30 +0.6
747,855 - Pf1N1B4_653 0.30 +0.0
747,858 + Pf1N1B4_653 0.30 +1.4
747,858 + Pf1N1B4_653 0.30 +0.0
747,858 + Pf1N1B4_653 0.30 +1.4
747,858 + Pf1N1B4_653 0.30 +1.4
747,945 + Pf1N1B4_653 0.45 -1.6
747,946 - Pf1N1B4_653 0.46 -1.6
747,946 - Pf1N1B4_653 0.46 -2.6
748,072 + Pf1N1B4_653 0.67 +0.4
748,072 + Pf1N1B4_653 0.67 +1.4
748,072 + Pf1N1B4_653 0.67 -0.3
748,072 + Pf1N1B4_653 0.67 -0.4
748,155 + Pf1N1B4_653 0.81 +0.0
748,155 + Pf1N1B4_653 0.81 -1.1
748,155 + Pf1N1B4_653 0.81 -0.3
748,155 + Pf1N1B4_653 0.81 -1.0
748,155 + Pf1N1B4_653 0.81 -2.3
748,155 + Pf1N1B4_653 0.81 -0.6
748,155 + Pf1N1B4_653 0.81 -1.6
748,156 - Pf1N1B4_653 0.82 +0.0
748,156 - Pf1N1B4_653 0.82 +0.3
748,156 - Pf1N1B4_653 0.82 +0.0
748,156 - Pf1N1B4_653 0.82 +0.2
748,157 + Pf1N1B4_653 0.82 +0.0
748,157 + Pf1N1B4_653 0.82 +0.6
748,157 + Pf1N1B4_653 0.82 +0.1
748,158 - Pf1N1B4_653 0.82 +0.0
748,158 - Pf1N1B4_653 0.82 -0.7
748,260 + -1.0
748,320 + +2.6
748,508 - Pf1N1B4_654 0.12 -0.4
748,525 + Pf1N1B4_654 0.14 -1.0
748,525 + Pf1N1B4_654 0.14 -1.0
748,525 + Pf1N1B4_654 0.14 +2.0
748,526 - Pf1N1B4_654 0.14 -0.6
748,526 - Pf1N1B4_654 0.14 +0.6
748,526 - Pf1N1B4_654 0.14 +3.0
748,526 - Pf1N1B4_654 0.14 -1.0

Or see this region's nucleotide sequence