Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_635 and Pf1N1B4_636 overlap by 11 nucleotides Pf1N1B4_636 and Pf1N1B4_637 are separated by 127 nucleotides Pf1N1B4_637 and Pf1N1B4_638 are separated by 0 nucleotides Pf1N1B4_638 and Pf1N1B4_639 are separated by 115 nucleotides
Pf1N1B4_635 - MotA/TolQ/ExbB proton channel family protein, at 731,637 to 732,332
_635
Pf1N1B4_636 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 732,322 to 732,789
_636
Pf1N1B4_637 - Holliday junction DNA helicase RuvB, at 732,917 to 733,978
_637
Pf1N1B4_638 - Holliday junction DNA helicase RuvA, at 733,979 to 734,596
_638
Pf1N1B4_639 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 734,712 to 735,236
_639
Position (kb) 732
733
734 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.392 kb on - strand, within Pf1N1B4_636 at 732.475 kb on - strand, within Pf1N1B4_636 at 732.533 kb on - strand, within Pf1N1B4_636 at 732.685 kb on - strand, within Pf1N1B4_636 at 732.685 kb on - strand, within Pf1N1B4_636 at 732.696 kb on - strand, within Pf1N1B4_636 at 732.705 kb on - strand, within Pf1N1B4_636 at 732.705 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.708 kb on - strand, within Pf1N1B4_636 at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.790 kb on - strand at 732.795 kb on - strand at 732.795 kb on - strand at 732.802 kb on - strand at 732.802 kb on - strand at 732.867 kb on + strand at 732.867 kb on + strand at 732.867 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.896 kb on + strand at 732.915 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.918 kb on + strand at 732.947 kb on + strand at 733.044 kb on + strand, within Pf1N1B4_637 at 733.062 kb on + strand, within Pf1N1B4_637 at 733.881 kb on + strand at 733.882 kb on - strand at 733.980 kb on + strand at 733.981 kb on - strand at 733.981 kb on - strand at 734.534 kb on + strand, within Pf1N1B4_638 at 734.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 732,392 - Pf1N1B4_636 0.15 +3.0 732,392 - Pf1N1B4_636 0.15 +0.8 732,392 - Pf1N1B4_636 0.15 -3.4 732,392 - Pf1N1B4_636 0.15 +2.6 732,475 - Pf1N1B4_636 0.33 +2.8 732,533 - Pf1N1B4_636 0.45 +2.6 732,685 - Pf1N1B4_636 0.78 +0.0 732,685 - Pf1N1B4_636 0.78 -1.0 732,696 - Pf1N1B4_636 0.80 +1.0 732,705 - Pf1N1B4_636 0.82 -0.6 732,705 - Pf1N1B4_636 0.82 +0.6 732,708 - Pf1N1B4_636 0.82 -3.4 732,708 - Pf1N1B4_636 0.82 +0.0 732,708 - Pf1N1B4_636 0.82 -2.6 732,708 - Pf1N1B4_636 0.82 -2.0 732,790 - +0.0 732,790 - +1.6 732,790 - +1.8 732,790 - +0.6 732,790 - -1.6 732,795 - -0.9 732,795 - -1.0 732,802 - +0.0 732,802 - +0.0 732,867 + +0.0 732,867 + -2.0 732,867 + -3.4 732,896 + -2.0 732,896 + -2.0 732,896 + -1.6 732,896 + -2.6 732,915 + -2.3 732,918 + -2.3 732,918 + -1.6 732,918 + -1.0 732,918 + -2.3 732,918 + -2.0 732,947 + -1.6 733,044 + Pf1N1B4_637 0.12 -1.0 733,062 + Pf1N1B4_637 0.14 -1.6 733,881 + -1.6 733,882 - -1.0 733,980 + -2.6 733,981 - -2.0 733,981 - -1.0 734,534 + Pf1N1B4_638 0.90 +0.0 734,711 - -1.6
Or see this region's nucleotide sequence