Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_6014

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_6012 and Pf1N1B4_6013 overlap by 4 nucleotidesPf1N1B4_6013 and Pf1N1B4_6014 overlap by 4 nucleotidesPf1N1B4_6014 and Pf1N1B4_6015 overlap by 4 nucleotides Pf1N1B4_6012 - COG0419: ATPase involved in DNA repair, at 472,823 to 475,912 _6012 Pf1N1B4_6013 - hypothetical protein, at 475,909 to 476,808 _6013 Pf1N1B4_6014 - ATP-dependent DNA helicase RecQ, at 476,805 to 479,408 _6014 Pf1N1B4_6015 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 479,405 to 482,761 _6015 Position (kb) 476 477 478 479 480Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 475.817 kb on + strandat 475.817 kb on + strandat 476.016 kb on + strand, within Pf1N1B4_6013at 476.017 kb on - strand, within Pf1N1B4_6013at 476.017 kb on - strand, within Pf1N1B4_6013at 476.120 kb on - strand, within Pf1N1B4_6013at 476.274 kb on - strand, within Pf1N1B4_6013at 476.334 kb on + strand, within Pf1N1B4_6013at 476.550 kb on + strand, within Pf1N1B4_6013at 476.588 kb on - strand, within Pf1N1B4_6013at 476.682 kb on + strand, within Pf1N1B4_6013at 476.708 kb on - strand, within Pf1N1B4_6013at 476.708 kb on - strand, within Pf1N1B4_6013at 476.709 kb on + strand, within Pf1N1B4_6013at 476.710 kb on - strand, within Pf1N1B4_6013at 476.710 kb on - strand, within Pf1N1B4_6013at 476.710 kb on - strand, within Pf1N1B4_6013at 476.797 kb on + strandat 476.797 kb on + strandat 476.802 kb on + strandat 476.814 kb on + strandat 476.839 kb on + strandat 476.839 kb on + strandat 476.914 kb on - strandat 476.917 kb on - strandat 476.917 kb on - strandat 476.917 kb on - strandat 476.917 kb on - strandat 476.949 kb on + strandat 476.949 kb on + strandat 476.950 kb on - strandat 476.950 kb on - strandat 476.950 kb on - strandat 476.970 kb on - strandat 477.028 kb on - strandat 477.042 kb on + strandat 477.043 kb on - strandat 477.071 kb on - strand, within Pf1N1B4_6014at 477.211 kb on + strand, within Pf1N1B4_6014at 477.220 kb on + strand, within Pf1N1B4_6014at 477.220 kb on + strandat 477.220 kb on + strand, within Pf1N1B4_6014at 477.221 kb on - strand, within Pf1N1B4_6014at 477.414 kb on - strand, within Pf1N1B4_6014at 477.504 kb on + strand, within Pf1N1B4_6014at 477.504 kb on + strand, within Pf1N1B4_6014at 477.504 kb on + strand, within Pf1N1B4_6014at 477.578 kb on + strand, within Pf1N1B4_6014at 477.578 kb on + strandat 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strandat 477.579 kb on - strand, within Pf1N1B4_6014at 477.725 kb on + strand, within Pf1N1B4_6014at 477.725 kb on + strand, within Pf1N1B4_6014at 477.726 kb on - strand, within Pf1N1B4_6014at 477.726 kb on - strand, within Pf1N1B4_6014at 477.835 kb on + strand, within Pf1N1B4_6014at 477.835 kb on + strand, within Pf1N1B4_6014at 477.835 kb on + strand, within Pf1N1B4_6014at 477.835 kb on + strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strand, within Pf1N1B4_6014at 477.836 kb on - strandat 477.839 kb on + strand, within Pf1N1B4_6014at 477.840 kb on - strandat 477.840 kb on - strand, within Pf1N1B4_6014at 477.840 kb on - strandat 477.840 kb on - strand, within Pf1N1B4_6014at 477.950 kb on + strand, within Pf1N1B4_6014at 477.950 kb on + strand, within Pf1N1B4_6014at 477.982 kb on + strand, within Pf1N1B4_6014at 477.982 kb on + strand, within Pf1N1B4_6014at 477.983 kb on - strand, within Pf1N1B4_6014at 477.983 kb on - strand, within Pf1N1B4_6014at 477.993 kb on + strand, within Pf1N1B4_6014at 478.041 kb on - strandat 478.204 kb on - strand, within Pf1N1B4_6014at 478.303 kb on + strand, within Pf1N1B4_6014at 478.304 kb on - strand, within Pf1N1B4_6014at 478.304 kb on - strand, within Pf1N1B4_6014at 478.304 kb on - strand, within Pf1N1B4_6014at 478.304 kb on - strand, within Pf1N1B4_6014at 478.304 kb on - strand, within Pf1N1B4_6014at 478.330 kb on - strand, within Pf1N1B4_6014at 478.330 kb on - strand, within Pf1N1B4_6014at 478.374 kb on + strand, within Pf1N1B4_6014at 478.375 kb on - strand, within Pf1N1B4_6014at 478.630 kb on - strand, within Pf1N1B4_6014at 478.696 kb on + strand, within Pf1N1B4_6014at 478.743 kb on + strandat 478.743 kb on + strand, within Pf1N1B4_6014at 478.743 kb on + strand, within Pf1N1B4_6014at 478.744 kb on - strand, within Pf1N1B4_6014at 478.744 kb on - strand, within Pf1N1B4_6014at 478.754 kb on + strand, within Pf1N1B4_6014at 478.828 kb on - strand, within Pf1N1B4_6014at 478.828 kb on - strand, within Pf1N1B4_6014at 478.831 kb on - strand, within Pf1N1B4_6014at 478.951 kb on + strand, within Pf1N1B4_6014at 478.951 kb on + strand, within Pf1N1B4_6014at 478.951 kb on + strand, within Pf1N1B4_6014at 478.952 kb on - strandat 478.952 kb on - strand, within Pf1N1B4_6014at 478.952 kb on - strand, within Pf1N1B4_6014at 478.955 kb on + strand, within Pf1N1B4_6014at 478.955 kb on + strand, within Pf1N1B4_6014at 478.955 kb on + strand, within Pf1N1B4_6014at 478.956 kb on - strand, within Pf1N1B4_6014at 478.959 kb on - strand, within Pf1N1B4_6014at 478.959 kb on - strand, within Pf1N1B4_6014at 479.043 kb on + strand, within Pf1N1B4_6014at 479.044 kb on - strandat 479.044 kb on - strand, within Pf1N1B4_6014at 479.044 kb on - strand, within Pf1N1B4_6014at 479.354 kb on + strandat 479.355 kb on - strandat 479.355 kb on - strandat 479.408 kb on + strandat 479.408 kb on + strandat 479.580 kb on - strandat 479.580 kb on - strandat 479.580 kb on - strandat 479.610 kb on + strandat 479.682 kb on - strandat 479.684 kb on + strandat 479.684 kb on + strandat 479.685 kb on - strandat 479.685 kb on - strandat 479.685 kb on - strandat 479.685 kb on - strandat 479.744 kb on - strand, within Pf1N1B4_6015at 479.775 kb on + strand, within Pf1N1B4_6015at 479.776 kb on - strand, within Pf1N1B4_6015at 479.776 kb on - strand, within Pf1N1B4_6015at 479.776 kb on - strand, within Pf1N1B4_6015at 479.827 kb on - strand, within Pf1N1B4_6015at 479.891 kb on + strand, within Pf1N1B4_6015at 479.891 kb on + strandat 479.892 kb on - strand, within Pf1N1B4_6015at 479.892 kb on - strand, within Pf1N1B4_6015at 479.892 kb on - strand, within Pf1N1B4_6015at 479.993 kb on - strand, within Pf1N1B4_6015at 480.090 kb on + strand, within Pf1N1B4_6015at 480.091 kb on - strand, within Pf1N1B4_6015at 480.091 kb on - strand, within Pf1N1B4_6015at 480.091 kb on - strand, within Pf1N1B4_6015at 480.178 kb on + strand, within Pf1N1B4_6015at 480.179 kb on - strand, within Pf1N1B4_6015at 480.205 kb on - strand, within Pf1N1B4_6015at 480.205 kb on - strand, within Pf1N1B4_6015at 480.254 kb on + strand, within Pf1N1B4_6015at 480.254 kb on + strand, within Pf1N1B4_6015at 480.254 kb on + strand, within Pf1N1B4_6015at 480.254 kb on + strand, within Pf1N1B4_6015at 480.255 kb on - strandat 480.255 kb on - strandat 480.273 kb on + strandat 480.275 kb on + strand, within Pf1N1B4_6015at 480.275 kb on + strand, within Pf1N1B4_6015at 480.295 kb on + strand, within Pf1N1B4_6015at 480.295 kb on + strand, within Pf1N1B4_6015at 480.330 kb on + strandat 480.335 kb on + strand, within Pf1N1B4_6015at 480.336 kb on - strand, within Pf1N1B4_6015at 480.387 kb on - strand, within Pf1N1B4_6015at 480.387 kb on - strand, within Pf1N1B4_6015

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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475,817 + +1.9
475,817 + +0.7
476,016 + Pf1N1B4_6013 0.12 -1.9
476,017 - Pf1N1B4_6013 0.12 +2.9
476,017 - Pf1N1B4_6013 0.12 -0.3
476,120 - Pf1N1B4_6013 0.23 -2.3
476,274 - Pf1N1B4_6013 0.41 +1.1
476,334 + Pf1N1B4_6013 0.47 +0.3
476,550 + Pf1N1B4_6013 0.71 -0.3
476,588 - Pf1N1B4_6013 0.75 -2.6
476,682 + Pf1N1B4_6013 0.86 +1.1
476,708 - Pf1N1B4_6013 0.89 +0.1
476,708 - Pf1N1B4_6013 0.89 +0.5
476,709 + Pf1N1B4_6013 0.89 -1.2
476,710 - Pf1N1B4_6013 0.89 +0.4
476,710 - Pf1N1B4_6013 0.89 -1.9
476,710 - Pf1N1B4_6013 0.89 -0.3
476,797 + -1.3
476,797 + -0.2
476,802 + +0.7
476,814 + +1.3
476,839 + -0.5
476,839 + +2.5
476,914 - -1.3
476,917 - +2.3
476,917 - +1.1
476,917 - -2.3
476,917 - -2.2
476,949 + -0.7
476,949 + +2.7
476,950 - +1.3
476,950 - +1.0
476,950 - +2.3
476,970 - -0.9
477,028 - -0.3
477,042 + -2.3
477,043 - -0.7
477,071 - Pf1N1B4_6014 0.10 -1.9
477,211 + Pf1N1B4_6014 0.16 +1.5
477,220 + Pf1N1B4_6014 0.16 -0.9
477,220 + -0.3
477,220 + Pf1N1B4_6014 0.16 -1.3
477,221 - Pf1N1B4_6014 0.16 +0.9
477,414 - Pf1N1B4_6014 0.23 -1.6
477,504 + Pf1N1B4_6014 0.27 -0.0
477,504 + Pf1N1B4_6014 0.27 +0.7
477,504 + Pf1N1B4_6014 0.27 -2.9
477,578 + Pf1N1B4_6014 0.30 +0.5
477,578 + -0.3
477,579 - Pf1N1B4_6014 0.30 +1.7
477,579 - Pf1N1B4_6014 0.30 +0.9
477,579 - Pf1N1B4_6014 0.30 +1.4
477,579 - Pf1N1B4_6014 0.30 -0.3
477,579 - -1.9
477,579 - Pf1N1B4_6014 0.30 -1.1
477,725 + Pf1N1B4_6014 0.35 +1.0
477,725 + Pf1N1B4_6014 0.35 +2.1
477,726 - Pf1N1B4_6014 0.35 -0.3
477,726 - Pf1N1B4_6014 0.35 -1.4
477,835 + Pf1N1B4_6014 0.40 +0.7
477,835 + Pf1N1B4_6014 0.40 +1.9
477,835 + Pf1N1B4_6014 0.40 +0.9
477,835 + Pf1N1B4_6014 0.40 +2.0
477,836 - Pf1N1B4_6014 0.40 -0.7
477,836 - Pf1N1B4_6014 0.40 -0.3
477,836 - Pf1N1B4_6014 0.40 +1.1
477,836 - Pf1N1B4_6014 0.40 +0.0
477,836 - Pf1N1B4_6014 0.40 +0.5
477,836 - Pf1N1B4_6014 0.40 +0.9
477,836 - Pf1N1B4_6014 0.40 +0.7
477,836 - Pf1N1B4_6014 0.40 -1.2
477,836 - -1.9
477,839 + Pf1N1B4_6014 0.40 -0.3
477,840 - -0.3
477,840 - Pf1N1B4_6014 0.40 -2.3
477,840 - +2.9
477,840 - Pf1N1B4_6014 0.40 -0.1
477,950 + Pf1N1B4_6014 0.44 +0.7
477,950 + Pf1N1B4_6014 0.44 -0.3
477,982 + Pf1N1B4_6014 0.45 +1.0
477,982 + Pf1N1B4_6014 0.45 -0.3
477,983 - Pf1N1B4_6014 0.45 +2.3
477,983 - Pf1N1B4_6014 0.45 -0.1
477,993 + Pf1N1B4_6014 0.46 +0.5
478,041 - +0.4
478,204 - Pf1N1B4_6014 0.54 +0.6
478,303 + Pf1N1B4_6014 0.58 -0.9
478,304 - Pf1N1B4_6014 0.58 +0.0
478,304 - Pf1N1B4_6014 0.58 -1.1
478,304 - Pf1N1B4_6014 0.58 +1.7
478,304 - Pf1N1B4_6014 0.58 +0.1
478,304 - Pf1N1B4_6014 0.58 -0.0
478,330 - Pf1N1B4_6014 0.59 +2.3
478,330 - Pf1N1B4_6014 0.59 -1.0
478,374 + Pf1N1B4_6014 0.60 +1.4
478,375 - Pf1N1B4_6014 0.60 -0.6
478,630 - Pf1N1B4_6014 0.70 +0.4
478,696 + Pf1N1B4_6014 0.73 +0.3
478,743 + +2.5
478,743 + Pf1N1B4_6014 0.74 +0.8
478,743 + Pf1N1B4_6014 0.74 -1.9
478,744 - Pf1N1B4_6014 0.74 +1.0
478,744 - Pf1N1B4_6014 0.74 -0.7
478,754 + Pf1N1B4_6014 0.75 +0.3
478,828 - Pf1N1B4_6014 0.78 -1.0
478,828 - Pf1N1B4_6014 0.78 +2.3
478,831 - Pf1N1B4_6014 0.78 -0.6
478,951 + Pf1N1B4_6014 0.82 +0.0
478,951 + Pf1N1B4_6014 0.82 -2.3
478,951 + Pf1N1B4_6014 0.82 -0.7
478,952 - -0.9
478,952 - Pf1N1B4_6014 0.82 +0.3
478,952 - Pf1N1B4_6014 0.82 +1.3
478,955 + Pf1N1B4_6014 0.83 -0.9
478,955 + Pf1N1B4_6014 0.83 +1.3
478,955 + Pf1N1B4_6014 0.83 -0.6
478,956 - Pf1N1B4_6014 0.83 +0.8
478,959 - Pf1N1B4_6014 0.83 -0.9
478,959 - Pf1N1B4_6014 0.83 +0.7
479,043 + Pf1N1B4_6014 0.86 -0.9
479,044 - +0.3
479,044 - Pf1N1B4_6014 0.86 +0.3
479,044 - Pf1N1B4_6014 0.86 -0.3
479,354 + -0.3
479,355 - -0.3
479,355 - -0.3
479,408 + -0.6
479,408 + -0.8
479,580 - +0.2
479,580 - -2.3
479,580 - +1.7
479,610 + +0.7
479,682 - +1.0
479,684 + +0.1
479,684 + -1.0
479,685 - -1.3
479,685 - -0.3
479,685 - +0.4
479,685 - -0.7
479,744 - Pf1N1B4_6015 0.10 +1.3
479,775 + Pf1N1B4_6015 0.11 -2.6
479,776 - Pf1N1B4_6015 0.11 -0.6
479,776 - Pf1N1B4_6015 0.11 +1.5
479,776 - Pf1N1B4_6015 0.11 +0.5
479,827 - Pf1N1B4_6015 0.13 -0.0
479,891 + Pf1N1B4_6015 0.14 +2.7
479,891 + +0.7
479,892 - Pf1N1B4_6015 0.15 +2.9
479,892 - Pf1N1B4_6015 0.15 -0.3
479,892 - Pf1N1B4_6015 0.15 +0.6
479,993 - Pf1N1B4_6015 0.18 -0.9
480,090 + Pf1N1B4_6015 0.20 +1.0
480,091 - Pf1N1B4_6015 0.20 +0.3
480,091 - Pf1N1B4_6015 0.20 -0.3
480,091 - Pf1N1B4_6015 0.20 -0.9
480,178 + Pf1N1B4_6015 0.23 -2.3
480,179 - Pf1N1B4_6015 0.23 +0.3
480,205 - Pf1N1B4_6015 0.24 +0.8
480,205 - Pf1N1B4_6015 0.24 +0.7
480,254 + Pf1N1B4_6015 0.25 +0.5
480,254 + Pf1N1B4_6015 0.25 -0.6
480,254 + Pf1N1B4_6015 0.25 -1.9
480,254 + Pf1N1B4_6015 0.25 -0.2
480,255 - -1.9
480,255 - -1.3
480,273 + -0.3
480,275 + Pf1N1B4_6015 0.26 -0.3
480,275 + Pf1N1B4_6015 0.26 -1.3
480,295 + Pf1N1B4_6015 0.27 +0.2
480,295 + Pf1N1B4_6015 0.27 +0.3
480,330 + +1.3
480,335 + Pf1N1B4_6015 0.28 -0.6
480,336 - Pf1N1B4_6015 0.28 -1.6
480,387 - Pf1N1B4_6015 0.29 -0.9
480,387 - Pf1N1B4_6015 0.29 -2.3

Or see this region's nucleotide sequence