Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5949

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5948 and Pf1N1B4_5949 are separated by 76 nucleotidesPf1N1B4_5949 and Pf1N1B4_5950 overlap by 4 nucleotidesPf1N1B4_5950 and Pf1N1B4_5951 are separated by 157 nucleotides Pf1N1B4_5948 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 397,793 to 398,626 _5948 Pf1N1B4_5949 - Phosphomannomutase (EC 5.4.2.8), at 398,703 to 399,134 _5949 Pf1N1B4_5950 - Transcriptional activator RfaH, at 399,131 to 399,637 _5950 Pf1N1B4_5951 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 399,795 to 400,631 _5951 Position (kb) 398 399 400Strain fitness (log2 ratio) -2 -1 0 1 2 3at 397.774 kb on + strandat 397.888 kb on + strand, within Pf1N1B4_5948at 397.888 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strandat 397.942 kb on + strand, within Pf1N1B4_5948at 397.942 kb on + strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 397.943 kb on - strand, within Pf1N1B4_5948at 398.101 kb on - strand, within Pf1N1B4_5948at 398.214 kb on + strand, within Pf1N1B4_5948at 398.238 kb on - strand, within Pf1N1B4_5948at 398.528 kb on + strand, within Pf1N1B4_5948at 398.528 kb on + strandat 398.528 kb on + strand, within Pf1N1B4_5948at 398.529 kb on - strand, within Pf1N1B4_5948at 398.573 kb on + strandat 398.573 kb on + strandat 398.574 kb on - strandat 398.574 kb on - strandat 398.574 kb on - strandat 398.679 kb on + strandat 398.679 kb on + strandat 398.682 kb on + strandat 398.702 kb on + strandat 398.762 kb on + strand, within Pf1N1B4_5949at 398.763 kb on - strand, within Pf1N1B4_5949at 398.778 kb on + strand, within Pf1N1B4_5949at 399.635 kb on + strandat 399.701 kb on - strandat 399.796 kb on + strandat 399.896 kb on - strand, within Pf1N1B4_5951at 399.899 kb on + strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 399.900 kb on - strand, within Pf1N1B4_5951at 400.013 kb on - strand, within Pf1N1B4_5951at 400.016 kb on + strand, within Pf1N1B4_5951at 400.016 kb on + strand, within Pf1N1B4_5951at 400.016 kb on + strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.017 kb on - strand, within Pf1N1B4_5951at 400.019 kb on - strand, within Pf1N1B4_5951at 400.019 kb on - strand, within Pf1N1B4_5951at 400.028 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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397,774 + +0.1
397,888 + Pf1N1B4_5948 0.11 +0.9
397,888 + Pf1N1B4_5948 0.11 +0.4
397,942 + Pf1N1B4_5948 0.18 +0.1
397,942 + Pf1N1B4_5948 0.18 +1.5
397,942 + Pf1N1B4_5948 0.18 -1.0
397,942 + -1.9
397,942 + Pf1N1B4_5948 0.18 +0.7
397,942 + Pf1N1B4_5948 0.18 +1.5
397,943 - Pf1N1B4_5948 0.18 +0.9
397,943 - Pf1N1B4_5948 0.18 +1.0
397,943 - Pf1N1B4_5948 0.18 -1.3
397,943 - Pf1N1B4_5948 0.18 -0.7
398,101 - Pf1N1B4_5948 0.37 -0.5
398,214 + Pf1N1B4_5948 0.50 -0.0
398,238 - Pf1N1B4_5948 0.53 -1.3
398,528 + Pf1N1B4_5948 0.88 -2.1
398,528 + +1.0
398,528 + Pf1N1B4_5948 0.88 -0.6
398,529 - Pf1N1B4_5948 0.88 +1.9
398,573 + +0.3
398,573 + +0.1
398,574 - -0.3
398,574 - +0.8
398,574 - +0.7
398,679 + +0.0
398,679 + -1.9
398,682 + -0.7
398,702 + +2.9
398,762 + Pf1N1B4_5949 0.14 +0.2
398,763 - Pf1N1B4_5949 0.14 -2.6
398,778 + Pf1N1B4_5949 0.17 -1.6
399,635 + +0.1
399,701 - -1.1
399,796 + -1.3
399,896 - Pf1N1B4_5951 0.12 -1.9
399,899 + Pf1N1B4_5951 0.12 -0.9
399,900 - Pf1N1B4_5951 0.13 +1.0
399,900 - Pf1N1B4_5951 0.13 -0.8
399,900 - Pf1N1B4_5951 0.13 -0.8
399,900 - Pf1N1B4_5951 0.13 -1.6
399,900 - Pf1N1B4_5951 0.13 +1.5
399,900 - Pf1N1B4_5951 0.13 -1.5
399,900 - Pf1N1B4_5951 0.13 -0.6
400,013 - Pf1N1B4_5951 0.26 +0.3
400,016 + Pf1N1B4_5951 0.26 +0.3
400,016 + Pf1N1B4_5951 0.26 +2.0
400,016 + Pf1N1B4_5951 0.26 +1.3
400,017 - Pf1N1B4_5951 0.27 +0.4
400,017 - Pf1N1B4_5951 0.27 -0.7
400,017 - Pf1N1B4_5951 0.27 -1.3
400,017 - Pf1N1B4_5951 0.27 -1.3
400,017 - Pf1N1B4_5951 0.27 +2.0
400,017 - Pf1N1B4_5951 0.27 -0.6
400,019 - Pf1N1B4_5951 0.27 +0.0
400,019 - Pf1N1B4_5951 0.27 +2.1
400,028 - -1.3

Or see this region's nucleotide sequence