Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5948 and Pf1N1B4_5949 are separated by 76 nucleotides Pf1N1B4_5949 and Pf1N1B4_5950 overlap by 4 nucleotides Pf1N1B4_5950 and Pf1N1B4_5951 are separated by 157 nucleotides
Pf1N1B4_5948 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 397,793 to 398,626
_5948
Pf1N1B4_5949 - Phosphomannomutase (EC 5.4.2.8), at 398,703 to 399,134
_5949
Pf1N1B4_5950 - Transcriptional activator RfaH, at 399,131 to 399,637
_5950
Pf1N1B4_5951 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 399,795 to 400,631
_5951
Position (kb) 398
399
400 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 397.774 kb on + strand at 397.888 kb on + strand, within Pf1N1B4_5948 at 397.888 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.942 kb on + strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 397.943 kb on - strand, within Pf1N1B4_5948 at 398.101 kb on - strand, within Pf1N1B4_5948 at 398.214 kb on + strand, within Pf1N1B4_5948 at 398.238 kb on - strand, within Pf1N1B4_5948 at 398.528 kb on + strand, within Pf1N1B4_5948 at 398.528 kb on + strand at 398.528 kb on + strand, within Pf1N1B4_5948 at 398.529 kb on - strand, within Pf1N1B4_5948 at 398.573 kb on + strand at 398.573 kb on + strand at 398.574 kb on - strand at 398.574 kb on - strand at 398.574 kb on - strand at 398.679 kb on + strand at 398.679 kb on + strand at 398.682 kb on + strand at 398.702 kb on + strand at 398.762 kb on + strand, within Pf1N1B4_5949 at 398.763 kb on - strand, within Pf1N1B4_5949 at 398.778 kb on + strand, within Pf1N1B4_5949 at 399.635 kb on + strand at 399.701 kb on - strand at 399.796 kb on + strand at 399.896 kb on - strand, within Pf1N1B4_5951 at 399.899 kb on + strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 399.900 kb on - strand, within Pf1N1B4_5951 at 400.013 kb on - strand, within Pf1N1B4_5951 at 400.016 kb on + strand, within Pf1N1B4_5951 at 400.016 kb on + strand, within Pf1N1B4_5951 at 400.016 kb on + strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.017 kb on - strand, within Pf1N1B4_5951 at 400.019 kb on - strand, within Pf1N1B4_5951 at 400.019 kb on - strand, within Pf1N1B4_5951 at 400.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 397,774 + +0.1 397,888 + Pf1N1B4_5948 0.11 +0.9 397,888 + Pf1N1B4_5948 0.11 +0.4 397,942 + Pf1N1B4_5948 0.18 +0.1 397,942 + Pf1N1B4_5948 0.18 +1.5 397,942 + Pf1N1B4_5948 0.18 -1.0 397,942 + -1.9 397,942 + Pf1N1B4_5948 0.18 +0.7 397,942 + Pf1N1B4_5948 0.18 +1.5 397,943 - Pf1N1B4_5948 0.18 +0.9 397,943 - Pf1N1B4_5948 0.18 +1.0 397,943 - Pf1N1B4_5948 0.18 -1.3 397,943 - Pf1N1B4_5948 0.18 -0.7 398,101 - Pf1N1B4_5948 0.37 -0.5 398,214 + Pf1N1B4_5948 0.50 -0.0 398,238 - Pf1N1B4_5948 0.53 -1.3 398,528 + Pf1N1B4_5948 0.88 -2.1 398,528 + +1.0 398,528 + Pf1N1B4_5948 0.88 -0.6 398,529 - Pf1N1B4_5948 0.88 +1.9 398,573 + +0.3 398,573 + +0.1 398,574 - -0.3 398,574 - +0.8 398,574 - +0.7 398,679 + +0.0 398,679 + -1.9 398,682 + -0.7 398,702 + +2.9 398,762 + Pf1N1B4_5949 0.14 +0.2 398,763 - Pf1N1B4_5949 0.14 -2.6 398,778 + Pf1N1B4_5949 0.17 -1.6 399,635 + +0.1 399,701 - -1.1 399,796 + -1.3 399,896 - Pf1N1B4_5951 0.12 -1.9 399,899 + Pf1N1B4_5951 0.12 -0.9 399,900 - Pf1N1B4_5951 0.13 +1.0 399,900 - Pf1N1B4_5951 0.13 -0.8 399,900 - Pf1N1B4_5951 0.13 -0.8 399,900 - Pf1N1B4_5951 0.13 -1.6 399,900 - Pf1N1B4_5951 0.13 +1.5 399,900 - Pf1N1B4_5951 0.13 -1.5 399,900 - Pf1N1B4_5951 0.13 -0.6 400,013 - Pf1N1B4_5951 0.26 +0.3 400,016 + Pf1N1B4_5951 0.26 +0.3 400,016 + Pf1N1B4_5951 0.26 +2.0 400,016 + Pf1N1B4_5951 0.26 +1.3 400,017 - Pf1N1B4_5951 0.27 +0.4 400,017 - Pf1N1B4_5951 0.27 -0.7 400,017 - Pf1N1B4_5951 0.27 -1.3 400,017 - Pf1N1B4_5951 0.27 -1.3 400,017 - Pf1N1B4_5951 0.27 +2.0 400,017 - Pf1N1B4_5951 0.27 -0.6 400,019 - Pf1N1B4_5951 0.27 +0.0 400,019 - Pf1N1B4_5951 0.27 +2.1 400,028 - -1.3
Or see this region's nucleotide sequence