Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5718 and Pf1N1B4_5719 are separated by 354 nucleotides Pf1N1B4_5719 and Pf1N1B4_5720 are separated by 67 nucleotides Pf1N1B4_5720 and Pf1N1B4_5721 are separated by 205 nucleotides Pf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotides
Pf1N1B4_5718 - Transcriptional regulator, Cro/CI family, at 161,223 to 162,014
_5718
Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043
_5719
Pf1N1B4_5720 - hypothetical protein, at 163,111 to 163,356
_5720
Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714
_5721
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Position (kb) 162
163
164 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 161.530 kb on + strand, within Pf1N1B4_5718 at 161.530 kb on + strand at 161.589 kb on + strand, within Pf1N1B4_5718 at 161.589 kb on + strand, within Pf1N1B4_5718 at 161.696 kb on + strand, within Pf1N1B4_5718 at 161.832 kb on + strand, within Pf1N1B4_5718 at 161.832 kb on + strand, within Pf1N1B4_5718 at 161.833 kb on - strand at 161.937 kb on + strand at 161.937 kb on + strand at 161.937 kb on + strand at 161.938 kb on - strand at 162.053 kb on + strand at 162.131 kb on - strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.298 kb on + strand at 162.298 kb on + strand at 162.304 kb on + strand at 162.305 kb on - strand at 162.305 kb on - strand at 162.355 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.391 kb on - strand at 162.413 kb on + strand at 162.413 kb on + strand at 162.759 kb on + strand, within Pf1N1B4_5719 at 162.760 kb on - strand, within Pf1N1B4_5719 at 162.876 kb on - strand, within Pf1N1B4_5719 at 162.957 kb on + strand, within Pf1N1B4_5719 at 162.958 kb on - strand at 163.056 kb on + strand at 163.058 kb on + strand at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.177 kb on - strand, within Pf1N1B4_5720 at 163.394 kb on - strand at 163.394 kb on - strand at 163.394 kb on - strand at 163.472 kb on + strand at 163.537 kb on - strand at 163.558 kb on + strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.568 kb on - strand at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.917 kb on - strand, within Pf1N1B4_5722 at 163.936 kb on - strand, within Pf1N1B4_5722
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 161,530 + Pf1N1B4_5718 0.39 +0.7 161,530 + -1.3 161,589 + Pf1N1B4_5718 0.46 +0.1 161,589 + Pf1N1B4_5718 0.46 +0.4 161,696 + Pf1N1B4_5718 0.60 -0.6 161,832 + Pf1N1B4_5718 0.77 +0.1 161,832 + Pf1N1B4_5718 0.77 -0.5 161,833 - -0.3 161,937 + +0.9 161,937 + -0.3 161,937 + -0.0 161,938 - -0.8 162,053 + -0.9 162,131 - -0.7 162,141 + +0.3 162,141 + -0.3 162,141 + +1.5 162,190 + +0.6 162,190 + +3.3 162,190 + +0.4 162,298 + -1.0 162,298 + +0.5 162,304 + +1.3 162,305 - -0.6 162,305 - -0.3 162,355 + -2.3 162,390 + +0.5 162,390 + -0.9 162,390 + -2.3 162,391 - +1.3 162,413 + -0.4 162,413 + -0.0 162,759 + Pf1N1B4_5719 0.58 +1.4 162,760 - Pf1N1B4_5719 0.58 +0.2 162,876 - Pf1N1B4_5719 0.75 +1.5 162,957 + Pf1N1B4_5719 0.87 +1.1 162,958 - -0.3 163,056 + +0.7 163,058 + +0.7 163,174 - Pf1N1B4_5720 0.26 -2.6 163,174 - Pf1N1B4_5720 0.26 -1.0 163,176 + Pf1N1B4_5720 0.26 -0.3 163,176 + Pf1N1B4_5720 0.26 +0.7 163,177 - Pf1N1B4_5720 0.27 +0.3 163,394 - -1.3 163,394 - +0.8 163,394 - -0.3 163,472 + -0.3 163,537 - -1.0 163,558 + -0.6 163,559 - +0.1 163,559 - -0.0 163,559 - +1.3 163,561 + +1.6 163,562 - -0.7 163,568 - -0.5 163,679 + Pf1N1B4_5721 0.76 +1.4 163,679 + Pf1N1B4_5721 0.76 +0.2 163,680 - Pf1N1B4_5721 0.77 +0.6 163,680 - Pf1N1B4_5721 0.77 -1.9 163,916 + Pf1N1B4_5722 0.54 -0.3 163,916 + Pf1N1B4_5722 0.54 -1.0 163,916 + Pf1N1B4_5722 0.54 -1.3 163,917 - Pf1N1B4_5722 0.54 +1.7 163,936 - Pf1N1B4_5722 0.59 +0.0
Or see this region's nucleotide sequence