Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5616 and Pf1N1B4_6052 are separated by 168 nucleotides Pf1N1B4_6052 and Pf1N1B4_5617 are separated by 63 nucleotides Pf1N1B4_5617 and Pf1N1B4_5618 are separated by 33 nucleotides Pf1N1B4_5618 and Pf1N1B4_5619 are separated by 2 nucleotides Pf1N1B4_5619 and Pf1N1B4_5620 are separated by 184 nucleotides
Pf1N1B4_5616 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 41,741 to 43,132
_5616
Pf1N1B4_6052 - tRNA-Gly-GCC, at 43,301 to 43,373
_6052
Pf1N1B4_5617 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 43,437 to 43,997
_5617
Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854
_5618
Pf1N1B4_5619 - BarA-associated response regulator UvrY (= GacA = SirA), at 45,857 to 46,441
_5619
Pf1N1B4_5620 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 46,626 to 47,069
_5620
Position (kb) 44
45
46 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 43.439 kb on - strand at 44.033 kb on - strand at 44.504 kb on + strand, within Pf1N1B4_5618 at 44.505 kb on - strand at 45.544 kb on + strand, within Pf1N1B4_5618 at 45.544 kb on + strand at 45.545 kb on - strand, within Pf1N1B4_5618 at 45.581 kb on - strand, within Pf1N1B4_5618 at 45.618 kb on + strand, within Pf1N1B4_5618 at 45.718 kb on + strand at 45.761 kb on - strand at 45.761 kb on - strand at 45.955 kb on + strand, within Pf1N1B4_5619 at 45.955 kb on + strand, within Pf1N1B4_5619 at 46.128 kb on + strand at 46.128 kb on + strand, within Pf1N1B4_5619 at 46.129 kb on - strand, within Pf1N1B4_5619 at 46.485 kb on - strand at 46.493 kb on - strand at 46.522 kb on + strand at 46.580 kb on + strand at 46.647 kb on - strand at 46.837 kb on - strand, within Pf1N1B4_5620
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 43,439 - -0.2 44,033 - +0.3 44,504 + Pf1N1B4_5618 0.26 -5.2 44,505 - -2.3 45,544 + Pf1N1B4_5618 0.83 -0.3 45,544 + -1.3 45,545 - Pf1N1B4_5618 0.83 -2.6 45,581 - Pf1N1B4_5618 0.85 -1.6 45,618 + Pf1N1B4_5618 0.87 -2.3 45,718 + -1.3 45,761 - -3.1 45,761 - -1.9 45,955 + Pf1N1B4_5619 0.17 -2.6 45,955 + Pf1N1B4_5619 0.17 -1.9 46,128 + +0.7 46,128 + Pf1N1B4_5619 0.46 -0.3 46,129 - Pf1N1B4_5619 0.46 -0.3 46,485 - -0.3 46,493 - -1.9 46,522 + -1.7 46,580 + -2.3 46,647 - -1.3 46,837 - Pf1N1B4_5620 0.48 -1.9
Or see this region's nucleotide sequence