Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5511 and Pf1N1B4_5512 are separated by 342 nucleotides Pf1N1B4_5512 and Pf1N1B4_5513 are separated by 75 nucleotides Pf1N1B4_5513 and Pf1N1B4_5514 are separated by 69 nucleotides
Pf1N1B4_5511 - hypothetical protein, at 260,474 to 260,755
_5511
Pf1N1B4_5512 - hypothetical protein, at 261,098 to 261,244
_5512
Pf1N1B4_5513 - hypothetical protein, at 261,320 to 261,517
_5513
Pf1N1B4_5514 - RecA protein, at 261,587 to 262,636
_5514
Position (kb)
261
262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 260.099 kb on + strand at 260.100 kb on - strand at 260.100 kb on - strand at 260.165 kb on + strand at 260.166 kb on - strand at 260.166 kb on - strand at 260.252 kb on + strand at 260.253 kb on - strand at 260.383 kb on + strand at 260.384 kb on - strand at 260.408 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.413 kb on + strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.414 kb on - strand at 260.476 kb on - strand at 260.476 kb on - strand at 260.476 kb on - strand at 260.499 kb on + strand at 260.499 kb on + strand at 260.500 kb on - strand at 260.510 kb on + strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.511 kb on - strand, within Pf1N1B4_5511 at 260.575 kb on - strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.639 kb on + strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.640 kb on - strand, within Pf1N1B4_5511 at 260.691 kb on - strand, within Pf1N1B4_5511 at 260.786 kb on + strand at 260.786 kb on + strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.787 kb on - strand at 260.790 kb on + strand at 260.920 kb on + strand at 260.920 kb on + strand at 261.018 kb on + strand at 261.018 kb on + strand at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.164 kb on - strand, within Pf1N1B4_5512 at 261.166 kb on - strand, within Pf1N1B4_5512 at 261.222 kb on - strand, within Pf1N1B4_5512 at 261.260 kb on + strand at 261.260 kb on + strand at 261.261 kb on - strand at 261.261 kb on - strand at 261.265 kb on + strand at 261.265 kb on + strand at 261.266 kb on - strand at 261.266 kb on - strand at 261.287 kb on - strand at 261.287 kb on - strand at 261.296 kb on - strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.321 kb on + strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.322 kb on - strand at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.458 kb on + strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.459 kb on - strand, within Pf1N1B4_5513 at 261.468 kb on + strand, within Pf1N1B4_5513 at 261.534 kb on + strand at 261.535 kb on - strand at 261.550 kb on - strand at 261.555 kb on - strand at 261.668 kb on - strand at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.712 kb on - strand, within Pf1N1B4_5514 at 261.756 kb on + strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.757 kb on - strand, within Pf1N1B4_5514 at 261.763 kb on - strand, within Pf1N1B4_5514 at 261.843 kb on + strand, within Pf1N1B4_5514 at 261.843 kb on + strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.844 kb on - strand, within Pf1N1B4_5514 at 261.847 kb on + strand at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.848 kb on - strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.981 kb on + strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514 at 261.982 kb on - strand, within Pf1N1B4_5514
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 260,099 + +0.3 260,100 - +1.7 260,100 - -0.7 260,165 + +0.1 260,166 - -0.1 260,166 - +0.9 260,252 + -0.7 260,253 - -1.9 260,383 + -0.5 260,384 - +1.4 260,408 + +0.5 260,413 + -0.6 260,413 + +0.5 260,413 + +1.7 260,413 + -1.5 260,414 - -0.1 260,414 - +0.1 260,414 - -0.5 260,414 - -2.2 260,414 - -0.2 260,414 - +0.7 260,414 - +1.1 260,414 - +2.4 260,476 - -2.5 260,476 - +0.1 260,476 - +0.1 260,499 + +0.1 260,499 + -1.1 260,500 - -0.3 260,510 + Pf1N1B4_5511 0.13 -1.9 260,511 - Pf1N1B4_5511 0.13 +0.5 260,511 - Pf1N1B4_5511 0.13 +1.8 260,511 - Pf1N1B4_5511 0.13 +1.4 260,511 - Pf1N1B4_5511 0.13 -1.5 260,575 - Pf1N1B4_5511 0.36 +1.1 260,639 + Pf1N1B4_5511 0.59 +0.8 260,639 + Pf1N1B4_5511 0.59 +0.1 260,640 - Pf1N1B4_5511 0.59 +0.1 260,640 - Pf1N1B4_5511 0.59 +1.3 260,691 - Pf1N1B4_5511 0.77 +0.1 260,786 + +0.4 260,786 + +0.7 260,787 - -0.3 260,787 - +1.7 260,787 - +0.0 260,787 - +0.1 260,790 + -1.1 260,920 + +1.6 260,920 + -1.5 261,018 + -3.5 261,018 + -1.3 261,164 - Pf1N1B4_5512 0.45 -1.6 261,164 - Pf1N1B4_5512 0.45 +2.4 261,166 - Pf1N1B4_5512 0.46 -2.2 261,222 - Pf1N1B4_5512 0.84 -0.1 261,260 + +1.4 261,260 + +0.7 261,261 - -1.5 261,261 - +1.1 261,265 + -1.5 261,265 + -1.5 261,266 - -0.5 261,266 - -0.9 261,287 - +0.7 261,287 - -1.5 261,296 - +2.2 261,316 + -0.5 261,316 + -3.1 261,317 - -1.5 261,317 - +1.4 261,321 + +0.1 261,321 + -0.8 261,321 + -0.6 261,322 - +0.1 261,322 - -0.9 261,322 - -0.5 261,322 - -0.9 261,458 + Pf1N1B4_5513 0.70 +0.3 261,458 + Pf1N1B4_5513 0.70 +1.5 261,459 - Pf1N1B4_5513 0.70 +0.7 261,459 - Pf1N1B4_5513 0.70 -1.6 261,459 - Pf1N1B4_5513 0.70 -0.9 261,468 + Pf1N1B4_5513 0.75 +0.4 261,534 + +0.7 261,535 - -2.1 261,550 - +1.3 261,555 - +0.7 261,668 - -1.7 261,712 - Pf1N1B4_5514 0.12 -0.6 261,712 - Pf1N1B4_5514 0.12 -1.9 261,712 - Pf1N1B4_5514 0.12 -0.0 261,712 - Pf1N1B4_5514 0.12 -0.7 261,712 - Pf1N1B4_5514 0.12 +0.1 261,712 - Pf1N1B4_5514 0.12 +0.9 261,712 - Pf1N1B4_5514 0.12 -0.4 261,756 + Pf1N1B4_5514 0.16 -1.5 261,757 - Pf1N1B4_5514 0.16 +2.4 261,757 - Pf1N1B4_5514 0.16 -0.8 261,757 - Pf1N1B4_5514 0.16 -1.9 261,757 - Pf1N1B4_5514 0.16 -1.9 261,757 - Pf1N1B4_5514 0.16 -0.3 261,757 - Pf1N1B4_5514 0.16 +0.2 261,757 - Pf1N1B4_5514 0.16 +0.7 261,763 - Pf1N1B4_5514 0.17 -0.4 261,843 + Pf1N1B4_5514 0.24 +1.7 261,843 + Pf1N1B4_5514 0.24 +1.8 261,844 - Pf1N1B4_5514 0.24 +1.7 261,844 - Pf1N1B4_5514 0.24 -1.5 261,844 - Pf1N1B4_5514 0.24 +0.5 261,844 - Pf1N1B4_5514 0.24 +0.4 261,844 - Pf1N1B4_5514 0.24 +0.5 261,847 + -0.5 261,848 - Pf1N1B4_5514 0.25 -1.9 261,848 - Pf1N1B4_5514 0.25 -1.2 261,848 - Pf1N1B4_5514 0.25 +0.8 261,848 - Pf1N1B4_5514 0.25 +0.9 261,981 + Pf1N1B4_5514 0.38 +1.1 261,981 + Pf1N1B4_5514 0.38 -0.4 261,981 + Pf1N1B4_5514 0.38 +0.7 261,981 + Pf1N1B4_5514 0.38 +0.1 261,981 + Pf1N1B4_5514 0.38 +1.1 261,981 + Pf1N1B4_5514 0.38 -0.9 261,982 - Pf1N1B4_5514 0.38 +2.1 261,982 - Pf1N1B4_5514 0.38 +1.4 261,982 - Pf1N1B4_5514 0.38 +1.4 261,982 - Pf1N1B4_5514 0.38 +0.9
Or see this region's nucleotide sequence