Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5504 and Pf1N1B4_5505 are separated by 38 nucleotides Pf1N1B4_5505 and Pf1N1B4_5506 overlap by 10 nucleotides Pf1N1B4_5506 and Pf1N1B4_5507 are separated by 48 nucleotides
Pf1N1B4_5504 - hypothetical protein, at 254,478 to 255,089
_5504
Pf1N1B4_5505 - hypothetical protein, at 255,128 to 255,304
_5505
Pf1N1B4_5506 - hypothetical protein, at 255,295 to 255,819
_5506
Pf1N1B4_5507 - hypothetical protein, at 255,868 to 256,542
_5507
Position (kb) 255
256
257 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 254.893 kb on + strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 254.894 kb on - strand, within Pf1N1B4_5504 at 255.054 kb on + strand at 255.055 kb on - strand at 255.066 kb on + strand at 255.067 kb on - strand at 255.067 kb on - strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.131 kb on + strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.132 kb on - strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.242 kb on + strand, within Pf1N1B4_5505 at 255.243 kb on - strand, within Pf1N1B4_5505 at 255.243 kb on - strand at 255.269 kb on + strand at 255.269 kb on + strand at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.269 kb on + strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.270 kb on - strand, within Pf1N1B4_5505 at 255.289 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.592 kb on + strand at 255.592 kb on + strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand, within Pf1N1B4_5506 at 255.593 kb on - strand at 255.593 kb on - strand at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.689 kb on + strand, within Pf1N1B4_5506 at 255.691 kb on + strand at 255.692 kb on - strand, within Pf1N1B4_5506 at 255.740 kb on + strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.741 kb on - strand, within Pf1N1B4_5506 at 255.829 kb on + strand at 255.829 kb on + strand at 255.829 kb on + strand at 255.830 kb on - strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.881 kb on + strand at 255.882 kb on - strand at 255.932 kb on + strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 255.933 kb on - strand at 256.353 kb on - strand, within Pf1N1B4_5507 at 256.405 kb on + strand, within Pf1N1B4_5507 at 256.405 kb on + strand, within Pf1N1B4_5507 at 256.406 kb on - strand, within Pf1N1B4_5507 at 256.490 kb on + strand at 256.491 kb on - strand at 256.491 kb on - strand at 256.662 kb on + strand at 256.662 kb on + strand at 256.680 kb on - strand at 256.720 kb on + strand at 256.720 kb on + strand at 256.721 kb on - strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.851 kb on + strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.852 kb on - strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.956 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.990 kb on + strand at 256.991 kb on - strand at 257.022 kb on + strand at 257.048 kb on + strand at 257.048 kb on + strand at 257.094 kb on + strand at 257.095 kb on - strand at 257.095 kb on - strand at 257.095 kb on - strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.134 kb on + strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.135 kb on - strand at 257.156 kb on - strand at 257.177 kb on - strand at 257.186 kb on - strand at 257.231 kb on - strand at 257.304 kb on - strand at 257.372 kb on + strand at 257.372 kb on + strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.373 kb on - strand at 257.389 kb on - strand at 257.389 kb on - strand at 257.389 kb on - strand at 257.421 kb on + strand at 257.422 kb on - strand at 257.422 kb on - strand at 257.466 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 254,893 + Pf1N1B4_5504 0.68 -1.5 254,894 - Pf1N1B4_5504 0.68 +1.4 254,894 - Pf1N1B4_5504 0.68 +1.7 255,054 + +0.9 255,055 - -0.9 255,066 + +2.1 255,067 - -0.3 255,067 - +1.0 255,131 + -1.5 255,131 + +1.1 255,131 + -0.5 255,131 + +0.5 255,131 + -2.4 255,131 + +0.4 255,131 + +0.1 255,132 - -0.3 255,132 - +0.1 255,132 - -1.5 255,132 - +0.1 255,132 - -0.2 255,132 - +0.8 255,132 - -0.3 255,242 + Pf1N1B4_5505 0.64 +2.1 255,242 + Pf1N1B4_5505 0.64 -2.5 255,242 + +0.1 255,242 + Pf1N1B4_5505 0.64 +0.7 255,242 + Pf1N1B4_5505 0.64 +0.1 255,242 + -0.9 255,242 + Pf1N1B4_5505 0.64 -1.4 255,242 + Pf1N1B4_5505 0.64 -0.5 255,242 + Pf1N1B4_5505 0.64 +0.9 255,243 - Pf1N1B4_5505 0.65 -1.4 255,243 - -1.5 255,269 + +0.1 255,269 + +0.1 255,269 + Pf1N1B4_5505 0.80 +0.8 255,269 + Pf1N1B4_5505 0.80 +0.1 255,270 - Pf1N1B4_5505 0.80 -1.9 255,270 - Pf1N1B4_5505 0.80 +2.8 255,289 + -2.9 255,592 + Pf1N1B4_5506 0.57 -0.8 255,592 + Pf1N1B4_5506 0.57 +1.7 255,592 + Pf1N1B4_5506 0.57 -0.3 255,592 + Pf1N1B4_5506 0.57 -0.5 255,592 + +0.1 255,592 + Pf1N1B4_5506 0.57 +1.1 255,593 - Pf1N1B4_5506 0.57 -0.4 255,593 - Pf1N1B4_5506 0.57 -0.2 255,593 - +0.1 255,593 - +1.1 255,689 + Pf1N1B4_5506 0.75 +0.3 255,689 + Pf1N1B4_5506 0.75 -0.1 255,689 + Pf1N1B4_5506 0.75 +0.6 255,689 + Pf1N1B4_5506 0.75 -1.5 255,691 + -0.9 255,692 - Pf1N1B4_5506 0.76 +1.8 255,740 + Pf1N1B4_5506 0.85 -0.9 255,741 - Pf1N1B4_5506 0.85 +0.3 255,741 - Pf1N1B4_5506 0.85 +0.3 255,829 + +0.4 255,829 + -0.9 255,829 + +0.1 255,830 - -1.5 255,881 + +0.1 255,881 + -2.7 255,881 + +0.1 255,882 - -1.5 255,932 + -2.1 255,933 - +0.3 255,933 - +0.4 255,933 - +0.4 255,933 - -2.5 255,933 - -0.2 255,933 - +0.5 256,353 - Pf1N1B4_5507 0.72 -0.6 256,405 + Pf1N1B4_5507 0.80 -1.5 256,405 + Pf1N1B4_5507 0.80 -1.9 256,406 - Pf1N1B4_5507 0.80 -1.5 256,490 + +0.5 256,491 - +0.9 256,491 - +0.1 256,662 + -0.9 256,662 + +1.7 256,680 - -1.6 256,720 + +0.7 256,720 + +0.1 256,721 - -0.4 256,851 + -1.2 256,851 + +0.1 256,851 + +0.5 256,851 + -1.1 256,851 + -1.7 256,851 + -1.5 256,851 + +0.1 256,851 + -1.5 256,851 + +2.4 256,851 + +0.7 256,851 + +1.2 256,852 - -1.5 256,852 - +0.1 256,852 - +0.5 256,852 - +0.4 256,852 - -2.2 256,956 + +0.5 256,956 + -0.9 256,956 + +0.4 256,956 + +0.1 256,990 + -0.1 256,990 + -1.9 256,990 + -1.9 256,991 - +0.8 257,022 + -1.1 257,048 + -0.2 257,048 + +0.5 257,094 + -0.3 257,095 - -0.7 257,095 - +1.9 257,095 - +1.1 257,134 + -1.1 257,134 + +0.1 257,134 + -0.5 257,134 + -0.9 257,135 - +0.6 257,135 - -0.6 257,135 - -0.2 257,135 - -2.9 257,135 - +0.4 257,135 - +0.4 257,135 - +0.8 257,156 - +1.7 257,177 - +0.1 257,186 - -0.1 257,231 - -1.9 257,304 - -0.9 257,372 + -0.9 257,372 + +0.1 257,373 - -1.1 257,373 - -1.2 257,373 - +0.1 257,373 - +1.7 257,373 - +0.7 257,373 - -2.7 257,373 - -0.8 257,389 - -1.0 257,389 - +1.7 257,389 - +0.1 257,421 + -1.7 257,422 - +0.1 257,422 - -0.5 257,466 + +2.3
Or see this region's nucleotide sequence