Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5331 and Pf1N1B4_5332 are separated by 2 nucleotides Pf1N1B4_5332 and Pf1N1B4_5333 are separated by 11 nucleotides Pf1N1B4_5333 and Pf1N1B4_5334 are separated by 131 nucleotides Pf1N1B4_5334 and Pf1N1B4_5335 are separated by 181 nucleotides Pf1N1B4_5335 and Pf1N1B4_5336 are separated by 448 nucleotides
Pf1N1B4_5331 - hypothetical protein, at 78,590 to 78,760
_5331
Pf1N1B4_5332 - hypothetical protein, at 78,763 to 78,957
_5332
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Pf1N1B4_5334 - hypothetical protein, at 79,727 to 79,930
_5334
Pf1N1B4_5335 - YebG, DNA damage-inducible gene in SOS regulon, expressed in stationary phase, at 80,112 to 80,399
_5335
Pf1N1B4_5336 - hypothetical protein, at 80,848 to 81,201
_5336
Position (kb)
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 78.729 kb on + strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.739 kb on - strand, within Pf1N1B4_5331 at 78.971 kb on - strand at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.106 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.268 kb on + strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.269 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.393 kb on - strand, within Pf1N1B4_5333 at 79.446 kb on - strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.501 kb on + strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.502 kb on - strand, within Pf1N1B4_5333 at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.576 kb on + strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.577 kb on - strand at 79.580 kb on + strand at 79.580 kb on + strand at 79.726 kb on - strand at 79.728 kb on + strand at 79.793 kb on + strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.794 kb on - strand, within Pf1N1B4_5334 at 79.797 kb on + strand, within Pf1N1B4_5334 at 80.045 kb on + strand at 80.045 kb on + strand at 80.045 kb on + strand at 80.046 kb on - strand at 80.147 kb on + strand, within Pf1N1B4_5335 at 80.147 kb on + strand, within Pf1N1B4_5335 at 80.148 kb on - strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.227 kb on + strand, within Pf1N1B4_5335 at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.228 kb on - strand at 80.228 kb on - strand, within Pf1N1B4_5335 at 80.348 kb on + strand, within Pf1N1B4_5335 at 80.348 kb on + strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.349 kb on - strand, within Pf1N1B4_5335 at 80.380 kb on + strand at 80.584 kb on - strand at 80.628 kb on + strand at 80.628 kb on + strand at 80.629 kb on - strand at 80.629 kb on - strand at 80.629 kb on - strand at 80.629 kb on - strand at 80.695 kb on + strand at 80.696 kb on - strand at 80.696 kb on - strand at 80.696 kb on - strand at 80.696 kb on - strand at 80.707 kb on - strand at 80.707 kb on - strand at 80.817 kb on + strand at 80.817 kb on + strand at 80.857 kb on + strand at 80.858 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 78,729 + Pf1N1B4_5331 0.81 +1.9 78,730 - Pf1N1B4_5331 0.82 -0.9 78,730 - Pf1N1B4_5331 0.82 +0.9 78,730 - Pf1N1B4_5331 0.82 +0.6 78,739 - Pf1N1B4_5331 0.87 -0.5 78,971 - +0.1 79,045 + Pf1N1B4_5333 0.12 +0.7 79,045 + Pf1N1B4_5333 0.12 +0.1 79,046 - Pf1N1B4_5333 0.12 +0.1 79,046 - Pf1N1B4_5333 0.12 -1.5 79,046 - Pf1N1B4_5333 0.12 -1.1 79,106 + Pf1N1B4_5333 0.22 +0.9 79,268 + Pf1N1B4_5333 0.48 +0.4 79,268 + Pf1N1B4_5333 0.48 +0.1 79,269 - Pf1N1B4_5333 0.48 +0.8 79,269 - Pf1N1B4_5333 0.48 -0.7 79,393 - Pf1N1B4_5333 0.68 -1.3 79,393 - Pf1N1B4_5333 0.68 -0.5 79,446 - Pf1N1B4_5333 0.76 +2.1 79,501 + Pf1N1B4_5333 0.85 +2.1 79,501 + Pf1N1B4_5333 0.85 +0.8 79,502 - Pf1N1B4_5333 0.85 -1.7 79,502 - Pf1N1B4_5333 0.85 +1.1 79,576 + +1.8 79,576 + -1.5 79,576 + +0.1 79,576 + -1.5 79,577 - +0.8 79,577 - -0.6 79,577 - +0.1 79,577 - -1.7 79,577 - +0.5 79,580 + -0.6 79,580 + +2.3 79,726 - +1.1 79,728 + +1.9 79,793 + Pf1N1B4_5334 0.32 -2.7 79,794 - Pf1N1B4_5334 0.33 -1.5 79,794 - Pf1N1B4_5334 0.33 +1.4 79,794 - Pf1N1B4_5334 0.33 +0.1 79,794 - Pf1N1B4_5334 0.33 -0.8 79,797 + Pf1N1B4_5334 0.34 -1.9 80,045 + +0.1 80,045 + -0.9 80,045 + -1.9 80,046 - +2.4 80,147 + Pf1N1B4_5335 0.12 +0.1 80,147 + Pf1N1B4_5335 0.12 +0.1 80,148 - Pf1N1B4_5335 0.12 -1.2 80,227 + Pf1N1B4_5335 0.40 +1.7 80,227 + +0.1 80,227 + Pf1N1B4_5335 0.40 -0.4 80,227 + Pf1N1B4_5335 0.40 +1.7 80,227 + Pf1N1B4_5335 0.40 +0.7 80,227 + Pf1N1B4_5335 0.40 +2.9 80,227 + Pf1N1B4_5335 0.40 -0.9 80,227 + Pf1N1B4_5335 0.40 -1.5 80,227 + Pf1N1B4_5335 0.40 +0.9 80,227 + Pf1N1B4_5335 0.40 -3.2 80,227 + Pf1N1B4_5335 0.40 +2.1 80,228 - Pf1N1B4_5335 0.40 +0.5 80,228 - Pf1N1B4_5335 0.40 -1.0 80,228 - -1.9 80,228 - Pf1N1B4_5335 0.40 +1.0 80,348 + Pf1N1B4_5335 0.82 +0.5 80,348 + Pf1N1B4_5335 0.82 -1.5 80,349 - Pf1N1B4_5335 0.82 +1.7 80,349 - Pf1N1B4_5335 0.82 -1.3 80,349 - Pf1N1B4_5335 0.82 -0.7 80,380 + -1.2 80,584 - -0.9 80,628 + +1.7 80,628 + -0.2 80,629 - -0.2 80,629 - +0.1 80,629 - +0.5 80,629 - -1.2 80,695 + +0.7 80,696 - -0.9 80,696 - +0.1 80,696 - -1.1 80,696 - -0.5 80,707 - -0.1 80,707 - +0.7 80,817 + -1.5 80,817 + +0.5 80,857 + +0.4 80,858 - +1.1
Or see this region's nucleotide sequence