Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5328 and Pf1N1B4_5329 are separated by 219 nucleotides Pf1N1B4_5329 and Pf1N1B4_5330 are separated by 25 nucleotides Pf1N1B4_5330 and Pf1N1B4_5331 are separated by 356 nucleotides Pf1N1B4_5331 and Pf1N1B4_5332 are separated by 2 nucleotides Pf1N1B4_5332 and Pf1N1B4_5333 are separated by 11 nucleotides
Pf1N1B4_5328 - hypothetical protein, at 76,949 to 77,134
_5328
Pf1N1B4_5329 - hypothetical protein, at 77,354 to 77,896
_5329
Pf1N1B4_5330 - hypothetical protein, at 77,922 to 78,233
_5330
Pf1N1B4_5331 - hypothetical protein, at 78,590 to 78,760
_5331
Pf1N1B4_5332 - hypothetical protein, at 78,763 to 78,957
_5332
Pf1N1B4_5333 - hypothetical protein, at 78,969 to 79,595
_5333
Position (kb) 77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 76.946 kb on - strand at 76.950 kb on + strand at 76.950 kb on + strand at 76.950 kb on + strand at 76.951 kb on - strand at 76.951 kb on - strand at 76.954 kb on + strand at 76.954 kb on + strand at 76.955 kb on - strand at 76.955 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.161 kb on - strand at 77.204 kb on - strand at 77.249 kb on + strand at 77.249 kb on + strand at 77.254 kb on + strand at 77.255 kb on - strand at 77.255 kb on - strand at 77.336 kb on + strand at 77.336 kb on + strand at 77.337 kb on - strand at 77.362 kb on + strand at 77.371 kb on + strand at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.410 kb on + strand, within Pf1N1B4_5329 at 77.411 kb on - strand, within Pf1N1B4_5329 at 77.686 kb on + strand, within Pf1N1B4_5329 at 77.752 kb on - strand, within Pf1N1B4_5329 at 77.781 kb on + strand, within Pf1N1B4_5329 at 77.781 kb on + strand at 77.782 kb on - strand, within Pf1N1B4_5329 at 77.847 kb on + strand at 77.923 kb on + strand at 77.923 kb on + strand at 77.980 kb on + strand, within Pf1N1B4_5330 at 78.022 kb on - strand, within Pf1N1B4_5330 at 78.152 kb on + strand at 78.152 kb on + strand, within Pf1N1B4_5330 at 78.153 kb on - strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.313 kb on + strand at 78.314 kb on - strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.336 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.392 kb on + strand at 78.392 kb on + strand at 78.425 kb on + strand at 78.425 kb on + strand at 78.426 kb on - strand at 78.426 kb on - strand at 78.474 kb on + strand at 78.474 kb on + strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.475 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.597 kb on - strand at 78.729 kb on + strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.730 kb on - strand, within Pf1N1B4_5331 at 78.739 kb on - strand, within Pf1N1B4_5331 at 78.971 kb on - strand at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.045 kb on + strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.046 kb on - strand, within Pf1N1B4_5333 at 79.106 kb on + strand, within Pf1N1B4_5333
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 76,946 - +0.2 76,950 + -1.3 76,950 + +0.9 76,950 + -3.2 76,951 - -0.1 76,951 - -0.5 76,954 + -2.6 76,954 + -0.9 76,955 - +1.1 76,955 - +1.7 77,161 - +0.4 77,161 - +0.7 77,161 - +0.3 77,161 - +0.5 77,204 - +0.4 77,249 + -1.5 77,249 + +0.7 77,254 + -1.5 77,255 - -0.0 77,255 - -1.9 77,336 + -0.3 77,336 + +0.1 77,337 - -1.6 77,362 + +0.8 77,371 + -1.6 77,410 + Pf1N1B4_5329 0.10 -1.5 77,410 + Pf1N1B4_5329 0.10 -0.7 77,410 + Pf1N1B4_5329 0.10 -0.3 77,410 + Pf1N1B4_5329 0.10 +0.9 77,411 - Pf1N1B4_5329 0.10 +2.1 77,686 + Pf1N1B4_5329 0.61 -0.6 77,752 - Pf1N1B4_5329 0.73 +2.0 77,781 + Pf1N1B4_5329 0.79 -1.0 77,781 + -0.9 77,782 - Pf1N1B4_5329 0.79 -1.2 77,847 + +0.7 77,923 + -2.1 77,923 + +0.1 77,980 + Pf1N1B4_5330 0.19 -0.7 78,022 - Pf1N1B4_5330 0.32 -1.5 78,152 + -1.9 78,152 + Pf1N1B4_5330 0.74 -0.3 78,153 - -0.9 78,313 + +1.3 78,313 + +0.7 78,313 + -2.2 78,313 + +1.9 78,314 - -1.2 78,336 + +0.8 78,336 + +1.1 78,336 + +0.4 78,336 + +0.5 78,351 + +1.9 78,351 + +0.1 78,351 + +0.2 78,352 - -1.2 78,352 - -1.5 78,392 + +1.3 78,392 + -0.3 78,425 + -1.2 78,425 + +3.1 78,426 - -2.1 78,426 - -0.6 78,474 + -0.5 78,474 + +0.1 78,475 - -0.3 78,475 - -1.2 78,475 - -2.2 78,475 - +0.1 78,475 - -2.5 78,597 - +1.1 78,597 - +0.7 78,597 - +1.8 78,729 + Pf1N1B4_5331 0.81 +1.9 78,730 - Pf1N1B4_5331 0.82 -0.9 78,730 - Pf1N1B4_5331 0.82 +0.9 78,730 - Pf1N1B4_5331 0.82 +0.6 78,739 - Pf1N1B4_5331 0.87 -0.5 78,971 - +0.1 79,045 + Pf1N1B4_5333 0.12 +0.7 79,045 + Pf1N1B4_5333 0.12 +0.1 79,046 - Pf1N1B4_5333 0.12 +0.1 79,046 - Pf1N1B4_5333 0.12 -1.5 79,046 - Pf1N1B4_5333 0.12 -1.1 79,106 + Pf1N1B4_5333 0.22 +0.9
Or see this region's nucleotide sequence