Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5239 and Pf1N1B4_5240 are separated by 173 nucleotides Pf1N1B4_5240 and Pf1N1B4_5241 are separated by 198 nucleotides Pf1N1B4_5241 and Pf1N1B4_5242 overlap by 8 nucleotides
Pf1N1B4_5239 - hypothetical protein, at 184 to 579
_5239
Pf1N1B4_5240 - hypothetical protein, at 753 to 872
_5240
Pf1N1B4_5241 - hypothetical protein, at 1,071 to 1,271
_5241
Pf1N1B4_5242 - hypothetical protein, at 1,264 to 1,524
_5242
Position (kb) 0
1 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 0.011 kb on + strand at 0.011 kb on + strand at 0.011 kb on + strand at 0.040 kb on + strand at 0.040 kb on + strand at 0.040 kb on + strand at 0.041 kb on - strand at 0.124 kb on + strand at 0.124 kb on + strand at 0.125 kb on - strand at 0.125 kb on - strand at 0.125 kb on - strand at 0.146 kb on - strand at 0.186 kb on - strand at 0.284 kb on + strand, within Pf1N1B4_5239 at 0.285 kb on - strand, within Pf1N1B4_5239 at 0.463 kb on - strand, within Pf1N1B4_5239 at 0.494 kb on + strand, within Pf1N1B4_5239 at 0.494 kb on + strand at 0.495 kb on - strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.496 kb on + strand, within Pf1N1B4_5239 at 0.497 kb on - strand, within Pf1N1B4_5239 at 0.597 kb on - strand at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.773 kb on - strand, within Pf1N1B4_5240 at 0.943 kb on + strand at 0.943 kb on + strand at 0.992 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.074 kb on + strand at 1.075 kb on - strand at 1.075 kb on - strand at 1.092 kb on - strand, within Pf1N1B4_5241 at 1.265 kb on + strand at 1.265 kb on + strand at 1.337 kb on - strand, within Pf1N1B4_5242 at 1.369 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.407 kb on + strand, within Pf1N1B4_5242 at 1.501 kb on + strand at 1.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 11 + -1.1 11 + +0.7 11 + -0.5 40 + -0.3 40 + +1.1 40 + +1.7 41 - +0.7 124 + +0.7 124 + -2.5 125 - -0.3 125 - -1.5 125 - +0.8 146 - -0.5 186 - -1.9 284 + Pf1N1B4_5239 0.25 +0.1 285 - Pf1N1B4_5239 0.26 -1.5 463 - Pf1N1B4_5239 0.70 +1.1 494 + Pf1N1B4_5239 0.78 +0.4 494 + +0.1 495 - Pf1N1B4_5239 0.79 +3.3 496 + Pf1N1B4_5239 0.79 +1.3 496 + Pf1N1B4_5239 0.79 -0.6 496 + Pf1N1B4_5239 0.79 +1.8 496 + Pf1N1B4_5239 0.79 -0.9 497 - Pf1N1B4_5239 0.79 +1.1 597 - -0.1 773 - Pf1N1B4_5240 0.17 +1.4 773 - Pf1N1B4_5240 0.17 +1.3 773 - Pf1N1B4_5240 0.17 -0.3 943 + -0.9 943 + +1.3 992 + +0.1 1,074 + -0.9 1,074 + +0.1 1,074 + -1.5 1,074 + -0.5 1,074 + +0.1 1,075 - +1.5 1,075 - +0.7 1,092 - Pf1N1B4_5241 0.10 +0.8 1,265 + +0.3 1,265 + +0.4 1,337 - Pf1N1B4_5242 0.28 +2.6 1,369 + Pf1N1B4_5242 0.40 -0.5 1,407 + Pf1N1B4_5242 0.55 -0.5 1,407 + Pf1N1B4_5242 0.55 +1.6 1,501 + -0.4 1,501 + +0.1
Or see this region's nucleotide sequence