Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5133

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5132 and Pf1N1B4_5133 are separated by 131 nucleotidesPf1N1B4_5133 and Pf1N1B4_5134 are separated by 13 nucleotidesPf1N1B4_5134 and Pf1N1B4_5135 overlap by 8 nucleotides Pf1N1B4_5132 - Transcriptional regulator, AraC family, at 5,710,848 to 5,711,864 _5132 Pf1N1B4_5133 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 5,711,996 to 5,713,180 _5133 Pf1N1B4_5134 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157), at 5,713,194 to 5,714,045 _5134 Pf1N1B4_5135 - Transcriptional regulator, TetR family, at 5,714,038 to 5,714,685 _5135 Position (kb) 5711 5712 5713 5714Strain fitness (log2 ratio) -2 -1 0 1at 5711.686 kb on - strandat 5711.742 kb on - strand, within Pf1N1B4_5132at 5711.827 kb on + strandat 5711.932 kb on - strandat 5711.954 kb on + strandat 5711.980 kb on + strandat 5711.980 kb on + strandat 5711.980 kb on + strandat 5711.981 kb on - strandat 5712.194 kb on + strand, within Pf1N1B4_5133at 5712.194 kb on + strand, within Pf1N1B4_5133at 5712.194 kb on + strand, within Pf1N1B4_5133at 5712.195 kb on - strand, within Pf1N1B4_5133at 5712.371 kb on + strand, within Pf1N1B4_5133at 5712.428 kb on + strand, within Pf1N1B4_5133at 5712.476 kb on - strandat 5712.635 kb on - strand, within Pf1N1B4_5133at 5713.718 kb on + strand, within Pf1N1B4_5134at 5713.936 kb on - strand, within Pf1N1B4_5134at 5714.038 kb on - strandat 5714.168 kb on - strand, within Pf1N1B4_5135

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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5,711,686 - -1.4
5,711,742 - Pf1N1B4_5132 0.88 +0.0
5,711,827 + -2.0
5,711,932 - -1.0
5,711,954 + +0.6
5,711,980 + +0.0
5,711,980 + -0.1
5,711,980 + -0.4
5,711,981 - -2.0
5,712,194 + Pf1N1B4_5133 0.17 -2.0
5,712,194 + Pf1N1B4_5133 0.17 -1.4
5,712,194 + Pf1N1B4_5133 0.17 -1.4
5,712,195 - Pf1N1B4_5133 0.17 -0.5
5,712,371 + Pf1N1B4_5133 0.32 +1.2
5,712,428 + Pf1N1B4_5133 0.36 -1.4
5,712,476 - +1.6
5,712,635 - Pf1N1B4_5133 0.54 -1.0
5,713,718 + Pf1N1B4_5134 0.62 -2.7
5,713,936 - Pf1N1B4_5134 0.87 -2.4
5,714,038 - -0.4
5,714,168 - Pf1N1B4_5135 0.20 -1.0

Or see this region's nucleotide sequence