Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5013

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5012 and Pf1N1B4_5013 are separated by 120 nucleotidesPf1N1B4_5013 and Pf1N1B4_5014 overlap by 4 nucleotides Pf1N1B4_5012 - Cointegrate resolution protein S, at 5,562,975 to 5,563,970 _5012 Pf1N1B4_5013 - Cointegrate resolution protein T, at 5,564,091 to 5,565,110 _5013 Pf1N1B4_5014 - Glutathione reductase (EC 1.8.1.7), at 5,565,107 to 5,566,465 _5014 Position (kb) 5564 5565 5566Strain fitness (log2 ratio) -2 -1 0 1 2at 5563.151 kb on - strand, within Pf1N1B4_5012at 5563.734 kb on + strand, within Pf1N1B4_5012at 5563.735 kb on - strand, within Pf1N1B4_5012at 5563.989 kb on + strandat 5563.989 kb on + strandat 5563.989 kb on + strandat 5563.990 kb on - strandat 5563.990 kb on - strandat 5563.991 kb on + strandat 5563.991 kb on + strandat 5564.058 kb on + strandat 5564.228 kb on + strand, within Pf1N1B4_5013at 5564.794 kb on + strand, within Pf1N1B4_5013at 5565.463 kb on - strandat 5565.546 kb on - strand, within Pf1N1B4_5014at 5565.578 kb on - strandat 5565.578 kb on - strand, within Pf1N1B4_5014at 5565.719 kb on - strand, within Pf1N1B4_5014at 5565.719 kb on - strand, within Pf1N1B4_5014at 5565.940 kb on - strand, within Pf1N1B4_5014

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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5,563,151 - Pf1N1B4_5012 0.18 -0.4
5,563,734 + Pf1N1B4_5012 0.76 +0.6
5,563,735 - Pf1N1B4_5012 0.76 -1.0
5,563,989 + -2.0
5,563,989 + +0.1
5,563,989 + +1.6
5,563,990 - -0.4
5,563,990 - +0.5
5,563,991 + -2.4
5,563,991 + -1.4
5,564,058 + -1.4
5,564,228 + Pf1N1B4_5013 0.13 -2.0
5,564,794 + Pf1N1B4_5013 0.69 -0.9
5,565,463 - -0.1
5,565,546 - Pf1N1B4_5014 0.32 -1.4
5,565,578 - -2.0
5,565,578 - Pf1N1B4_5014 0.35 -2.0
5,565,719 - Pf1N1B4_5014 0.45 -1.8
5,565,719 - Pf1N1B4_5014 0.45 +1.9
5,565,940 - Pf1N1B4_5014 0.61 +0.0

Or see this region's nucleotide sequence