Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4992 and Pf1N1B4_4993 are separated by 140 nucleotides Pf1N1B4_4993 and Pf1N1B4_4994 are separated by 168 nucleotides
Pf1N1B4_4992 - Dienelactone hydrolase and related enzymes, at 5,538,604 to 5,539,563
_4992
Pf1N1B4_4993 - Arabinose efflux permease, at 5,539,704 to 5,540,849
_4993
Pf1N1B4_4994 - Putrescine/proton symporter, putrescine/ornithine antiporter PotE, at 5,541,018 to 5,542,349
_4994
Position (kb) 5539
5540
5541 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5538.797 kb on - strand, within Pf1N1B4_4992 at 5538.901 kb on + strand, within Pf1N1B4_4992 at 5539.033 kb on - strand at 5539.061 kb on - strand, within Pf1N1B4_4992 at 5539.061 kb on - strand, within Pf1N1B4_4992 at 5539.061 kb on - strand at 5539.061 kb on - strand at 5539.238 kb on - strand at 5539.343 kb on - strand, within Pf1N1B4_4992 at 5539.343 kb on - strand, within Pf1N1B4_4992 at 5539.436 kb on - strand, within Pf1N1B4_4992 at 5539.436 kb on - strand, within Pf1N1B4_4992 at 5539.633 kb on - strand at 5539.636 kb on - strand at 5539.679 kb on + strand at 5539.679 kb on + strand at 5539.781 kb on - strand at 5539.914 kb on - strand, within Pf1N1B4_4993 at 5539.953 kb on + strand, within Pf1N1B4_4993 at 5539.954 kb on - strand, within Pf1N1B4_4993 at 5539.996 kb on + strand, within Pf1N1B4_4993 at 5540.198 kb on - strand, within Pf1N1B4_4993 at 5540.343 kb on + strand, within Pf1N1B4_4993 at 5540.950 kb on + strand at 5541.208 kb on - strand, within Pf1N1B4_4994 at 5541.208 kb on - strand, within Pf1N1B4_4994 at 5541.208 kb on - strand, within Pf1N1B4_4994 at 5541.283 kb on - strand, within Pf1N1B4_4994 at 5541.288 kb on + strand, within Pf1N1B4_4994 at 5541.289 kb on - strand, within Pf1N1B4_4994 at 5541.593 kb on + strand, within Pf1N1B4_4994 at 5541.596 kb on + strand, within Pf1N1B4_4994 at 5541.596 kb on + strand, within Pf1N1B4_4994 at 5541.597 kb on - strand, within Pf1N1B4_4994 at 5541.612 kb on - strand, within Pf1N1B4_4994 at 5541.729 kb on + strand, within Pf1N1B4_4994 at 5541.811 kb on - strand at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.818 kb on + strand, within Pf1N1B4_4994 at 5541.819 kb on - strand, within Pf1N1B4_4994 at 5541.819 kb on - strand, within Pf1N1B4_4994 at 5541.819 kb on - strand, within Pf1N1B4_4994 at 5541.819 kb on - strand, within Pf1N1B4_4994 at 5541.819 kb on - strand at 5541.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 5,538,797 - Pf1N1B4_4992 0.20 -1.4 5,538,901 + Pf1N1B4_4992 0.31 -1.4 5,539,033 - -2.4 5,539,061 - Pf1N1B4_4992 0.48 +0.2 5,539,061 - Pf1N1B4_4992 0.48 +0.6 5,539,061 - +1.8 5,539,061 - -1.4 5,539,238 - +2.2 5,539,343 - Pf1N1B4_4992 0.77 -0.5 5,539,343 - Pf1N1B4_4992 0.77 -0.4 5,539,436 - Pf1N1B4_4992 0.87 -1.4 5,539,436 - Pf1N1B4_4992 0.87 -1.4 5,539,633 - +0.2 5,539,636 - +1.5 5,539,679 + +0.6 5,539,679 + +1.9 5,539,781 - +0.2 5,539,914 - Pf1N1B4_4993 0.18 -0.4 5,539,953 + Pf1N1B4_4993 0.22 -1.4 5,539,954 - Pf1N1B4_4993 0.22 -1.4 5,539,996 + Pf1N1B4_4993 0.25 -2.0 5,540,198 - Pf1N1B4_4993 0.43 -0.1 5,540,343 + Pf1N1B4_4993 0.56 -1.0 5,540,950 + -0.2 5,541,208 - Pf1N1B4_4994 0.14 +0.6 5,541,208 - Pf1N1B4_4994 0.14 +0.3 5,541,208 - Pf1N1B4_4994 0.14 -0.4 5,541,283 - Pf1N1B4_4994 0.20 -0.7 5,541,288 + Pf1N1B4_4994 0.20 +0.9 5,541,289 - Pf1N1B4_4994 0.20 -0.8 5,541,593 + Pf1N1B4_4994 0.43 +0.0 5,541,596 + Pf1N1B4_4994 0.43 -0.8 5,541,596 + Pf1N1B4_4994 0.43 +0.4 5,541,597 - Pf1N1B4_4994 0.43 -1.7 5,541,612 - Pf1N1B4_4994 0.45 -0.8 5,541,729 + Pf1N1B4_4994 0.53 +1.8 5,541,811 - -1.4 5,541,818 + Pf1N1B4_4994 0.60 +2.3 5,541,818 + Pf1N1B4_4994 0.60 -0.8 5,541,818 + Pf1N1B4_4994 0.60 +0.3 5,541,818 + Pf1N1B4_4994 0.60 -0.4 5,541,818 + Pf1N1B4_4994 0.60 +0.3 5,541,818 + Pf1N1B4_4994 0.60 +0.9 5,541,818 + Pf1N1B4_4994 0.60 -1.0 5,541,818 + Pf1N1B4_4994 0.60 +0.6 5,541,818 + Pf1N1B4_4994 0.60 -2.7 5,541,819 - Pf1N1B4_4994 0.60 -1.4 5,541,819 - Pf1N1B4_4994 0.60 +1.2 5,541,819 - Pf1N1B4_4994 0.60 +0.0 5,541,819 - Pf1N1B4_4994 0.60 -0.4 5,541,819 - +1.2 5,541,819 - -0.4
Or see this region's nucleotide sequence