Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4932 and Pf1N1B4_4933 are separated by 171 nucleotides Pf1N1B4_4933 and Pf1N1B4_4934 are separated by 224 nucleotides Pf1N1B4_4934 and Pf1N1B4_4935 overlap by 4 nucleotides
Pf1N1B4_4932 - Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83), at 5,466,700 to 5,467,779
_4932
Pf1N1B4_4933 - FIG00955097: hypothetical protein, at 5,467,951 to 5,468,175
_4933
Pf1N1B4_4934 - DNA damage response nuclease (from data) , at 5,468,400 to 5,470,052
_4934
Pf1N1B4_4935 - DinG family ATP-dependent helicase CPE1197, at 5,470,049 to 5,472,328
_4935
Position (kb)
5468
5469
5470
5471 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.567 kb on + strand, within Pf1N1B4_4932 at 5467.568 kb on - strand, within Pf1N1B4_4932 at 5467.926 kb on - strand at 5468.072 kb on + strand at 5468.175 kb on + strand at 5468.176 kb on - strand at 5468.347 kb on - strand at 5468.347 kb on - strand at 5468.347 kb on - strand at 5468.353 kb on + strand at 5468.408 kb on - strand at 5468.408 kb on - strand at 5468.488 kb on + strand at 5468.610 kb on + strand, within Pf1N1B4_4934 at 5468.611 kb on - strand, within Pf1N1B4_4934 at 5468.963 kb on + strand, within Pf1N1B4_4934 at 5468.964 kb on - strand, within Pf1N1B4_4934 at 5469.349 kb on + strand, within Pf1N1B4_4934 at 5469.519 kb on + strand, within Pf1N1B4_4934 at 5469.519 kb on + strand at 5469.520 kb on - strand, within Pf1N1B4_4934 at 5469.705 kb on + strand, within Pf1N1B4_4934 at 5469.706 kb on - strand, within Pf1N1B4_4934 at 5469.706 kb on - strand, within Pf1N1B4_4934 at 5469.730 kb on - strand at 5469.764 kb on - strand at 5470.055 kb on + strand at 5470.295 kb on + strand, within Pf1N1B4_4935 at 5470.296 kb on - strand at 5470.361 kb on + strand, within Pf1N1B4_4935 at 5470.479 kb on + strand at 5470.479 kb on + strand, within Pf1N1B4_4935 at 5470.929 kb on - strand at 5471.031 kb on - strand at 5471.031 kb on - strand, within Pf1N1B4_4935
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 5,467,567 + Pf1N1B4_4932 0.80 +0.3 5,467,567 + Pf1N1B4_4932 0.80 -1.4 5,467,568 - Pf1N1B4_4932 0.80 -0.4 5,467,926 - +0.6 5,468,072 + +1.2 5,468,175 + +0.3 5,468,176 - +1.9 5,468,347 - -1.4 5,468,347 - -0.4 5,468,347 - -3.0 5,468,353 + +0.9 5,468,408 - -2.4 5,468,408 - -0.2 5,468,488 + -2.0 5,468,610 + Pf1N1B4_4934 0.13 -1.4 5,468,611 - Pf1N1B4_4934 0.13 -1.4 5,468,963 + Pf1N1B4_4934 0.34 -2.2 5,468,964 - Pf1N1B4_4934 0.34 +0.6 5,469,349 + Pf1N1B4_4934 0.57 -1.4 5,469,519 + Pf1N1B4_4934 0.68 -2.1 5,469,519 + -0.4 5,469,520 - Pf1N1B4_4934 0.68 -2.1 5,469,705 + Pf1N1B4_4934 0.79 -2.0 5,469,706 - Pf1N1B4_4934 0.79 -0.4 5,469,706 - Pf1N1B4_4934 0.79 -1.0 5,469,730 - -0.4 5,469,764 - -1.4 5,470,055 + -0.4 5,470,295 + Pf1N1B4_4935 0.11 -0.4 5,470,296 - -1.4 5,470,361 + Pf1N1B4_4935 0.14 -3.0 5,470,479 + +0.6 5,470,479 + Pf1N1B4_4935 0.19 -2.2 5,470,929 - +0.2 5,471,031 - -0.4 5,471,031 - Pf1N1B4_4935 0.43 -0.4
Or see this region's nucleotide sequence