Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_45 and Pf1N1B4_46 are separated by 55 nucleotides Pf1N1B4_46 and Pf1N1B4_47 are separated by 3 nucleotides Pf1N1B4_47 and Pf1N1B4_48 are separated by 145 nucleotides Pf1N1B4_48 and Pf1N1B4_49 are separated by 30 nucleotides Pf1N1B4_49 and Pf1N1B4_50 are separated by 163 nucleotides
Pf1N1B4_45 - Copper resistance protein B, at 53,588 to 54,478
_45
Pf1N1B4_46 - Copper resistance protein C precursor, at 54,534 to 54,917
_46
Pf1N1B4_47 - Copper resistance protein D, at 54,921 to 55,850
_47
Pf1N1B4_48 - hypothetical protein, at 55,996 to 56,406
_48
Pf1N1B4_49 - hypothetical protein, at 56,437 to 56,643
_49
Pf1N1B4_50 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 56,807 to 56,989
_50
Position (kb) 54
55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 54.137 kb on + strand, within Pf1N1B4_45 at 54.138 kb on - strand, within Pf1N1B4_45 at 54.210 kb on - strand, within Pf1N1B4_45 at 54.365 kb on + strand at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.365 kb on + strand, within Pf1N1B4_45 at 54.366 kb on - strand, within Pf1N1B4_45 at 54.366 kb on - strand, within Pf1N1B4_45 at 54.366 kb on - strand, within Pf1N1B4_45 at 54.393 kb on - strand at 54.410 kb on + strand at 54.411 kb on - strand at 54.485 kb on + strand at 54.486 kb on - strand at 54.514 kb on - strand at 54.843 kb on + strand, within Pf1N1B4_46 at 54.843 kb on + strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.844 kb on - strand, within Pf1N1B4_46 at 54.899 kb on + strand at 54.899 kb on + strand at 54.899 kb on + strand at 54.899 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.960 kb on + strand at 54.961 kb on - strand at 54.961 kb on - strand at 54.961 kb on - strand at 54.961 kb on - strand at 54.961 kb on - strand at 55.006 kb on - strand at 55.006 kb on - strand at 55.014 kb on - strand, within Pf1N1B4_47 at 55.276 kb on + strand, within Pf1N1B4_47 at 55.277 kb on - strand, within Pf1N1B4_47 at 55.341 kb on - strand, within Pf1N1B4_47 at 55.499 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.611 kb on + strand, within Pf1N1B4_47 at 55.612 kb on - strand, within Pf1N1B4_47 at 55.848 kb on + strand at 55.848 kb on + strand at 55.863 kb on + strand at 55.863 kb on + strand at 55.864 kb on - strand at 55.870 kb on + strand at 55.980 kb on + strand at 55.980 kb on + strand at 55.981 kb on - strand at 56.005 kb on + strand at 56.006 kb on - strand at 56.516 kb on + strand, within Pf1N1B4_49 at 56.517 kb on - strand, within Pf1N1B4_49 at 56.520 kb on - strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.560 kb on - strand, within Pf1N1B4_49 at 56.560 kb on - strand at 56.560 kb on - strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.767 kb on + strand at 56.784 kb on + strand at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 54,137 + Pf1N1B4_45 0.62 -0.6 54,138 - Pf1N1B4_45 0.62 -1.1 54,210 - Pf1N1B4_45 0.70 -0.5 54,365 + +1.4 54,365 + Pf1N1B4_45 0.87 +0.5 54,365 + Pf1N1B4_45 0.87 -1.5 54,365 + Pf1N1B4_45 0.87 +0.4 54,365 + Pf1N1B4_45 0.87 +0.8 54,365 + Pf1N1B4_45 0.87 -0.6 54,365 + Pf1N1B4_45 0.87 +0.0 54,366 - Pf1N1B4_45 0.87 -0.2 54,366 - Pf1N1B4_45 0.87 -2.3 54,366 - Pf1N1B4_45 0.87 +0.4 54,393 - -2.0 54,410 + -1.8 54,411 - -1.2 54,485 + -1.2 54,486 - -0.2 54,514 - -0.2 54,843 + Pf1N1B4_46 0.80 +1.3 54,843 + Pf1N1B4_46 0.80 -0.2 54,844 - Pf1N1B4_46 0.81 +1.1 54,844 - Pf1N1B4_46 0.81 +1.4 54,844 - Pf1N1B4_46 0.81 -1.7 54,844 - Pf1N1B4_46 0.81 -0.8 54,899 + +1.1 54,899 + +2.5 54,899 + -0.2 54,899 + +1.8 54,960 + +1.1 54,960 + +0.2 54,960 + +0.8 54,960 + -0.2 54,960 + +0.5 54,960 + -1.0 54,960 + -2.8 54,961 - -0.2 54,961 - -1.8 54,961 - +0.8 54,961 - -1.8 54,961 - -0.2 55,006 - -3.4 55,006 - -0.6 55,014 - Pf1N1B4_47 0.10 -0.8 55,276 + Pf1N1B4_47 0.38 -2.8 55,277 - Pf1N1B4_47 0.38 -1.2 55,341 - Pf1N1B4_47 0.45 +1.1 55,499 + Pf1N1B4_47 0.62 -0.2 55,611 + Pf1N1B4_47 0.74 -0.2 55,611 + Pf1N1B4_47 0.74 -2.2 55,611 + Pf1N1B4_47 0.74 -0.2 55,611 + Pf1N1B4_47 0.74 +0.3 55,612 - Pf1N1B4_47 0.74 -2.5 55,848 + +1.9 55,848 + -0.2 55,863 + +0.4 55,863 + +0.0 55,864 - -0.2 55,870 + -0.2 55,980 + -0.6 55,980 + -0.2 55,981 - -2.4 56,005 + +0.2 56,006 - -0.6 56,516 + Pf1N1B4_49 0.38 -2.8 56,517 - Pf1N1B4_49 0.39 -0.2 56,520 - Pf1N1B4_49 0.40 +1.8 56,559 + Pf1N1B4_49 0.59 +1.4 56,559 + Pf1N1B4_49 0.59 +1.0 56,560 - Pf1N1B4_49 0.59 -0.2 56,560 - -0.2 56,560 - Pf1N1B4_49 0.59 -3.4 56,588 + Pf1N1B4_49 0.73 -0.8 56,588 + Pf1N1B4_49 0.73 -1.1 56,767 + +0.0 56,784 + -0.8 56,837 + Pf1N1B4_50 0.16 +1.6 56,837 + Pf1N1B4_50 0.16 +0.8 56,837 + Pf1N1B4_50 0.16 +0.5 56,837 + Pf1N1B4_50 0.16 -0.2 56,838 - Pf1N1B4_50 0.17 -0.7 56,838 - Pf1N1B4_50 0.17 -3.0 56,838 - Pf1N1B4_50 0.17 +0.8 56,838 - +0.8
Or see this region's nucleotide sequence