Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_464 and Pf1N1B4_465 overlap by 4 nucleotides Pf1N1B4_465 and Pf1N1B4_466 are separated by 232 nucleotides Pf1N1B4_466 and Pf1N1B4_467 overlap by 4 nucleotides Pf1N1B4_467 and Pf1N1B4_468 overlap by 1 nucleotides
Pf1N1B4_464 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 555,443 to 557,764
_464
Pf1N1B4_465 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 557,761 to 558,234
_465
Pf1N1B4_466 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 558,467 to 559,486
_466
Pf1N1B4_467 - Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48), at 559,483 to 559,947
_467
Pf1N1B4_468 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 559,947 to 560,711
_468
Position (kb) 558
559
560 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 557.574 kb on + strand at 557.574 kb on + strand at 557.575 kb on - strand at 557.575 kb on - strand at 557.575 kb on - strand at 557.582 kb on - strand at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand, within Pf1N1B4_465 at 557.823 kb on - strand, within Pf1N1B4_465 at 557.823 kb on - strand, within Pf1N1B4_465 at 558.013 kb on + strand at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.019 kb on - strand, within Pf1N1B4_465 at 558.093 kb on + strand, within Pf1N1B4_465 at 558.093 kb on + strand, within Pf1N1B4_465 at 558.190 kb on + strand at 558.191 kb on - strand at 558.203 kb on + strand at 558.203 kb on + strand at 558.204 kb on - strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.229 kb on - strand at 558.229 kb on - strand at 558.229 kb on - strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.249 kb on - strand at 558.249 kb on - strand at 558.249 kb on - strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.255 kb on - strand at 558.255 kb on - strand at 558.255 kb on - strand at 558.326 kb on - strand at 558.388 kb on + strand at 558.393 kb on + strand at 558.393 kb on + strand at 559.544 kb on - strand, within Pf1N1B4_467 at 559.707 kb on - strand, within Pf1N1B4_467 at 559.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 557,574 + -2.8 557,574 + -0.6 557,575 - +3.1 557,575 - -0.5 557,575 - +0.8 557,582 - +0.5 557,822 + Pf1N1B4_465 0.13 +0.2 557,822 + Pf1N1B4_465 0.13 +2.3 557,822 + +2.5 557,822 + Pf1N1B4_465 0.13 +0.8 557,822 + Pf1N1B4_465 0.13 +1.0 557,823 - Pf1N1B4_465 0.13 +0.5 557,823 - Pf1N1B4_465 0.13 +0.5 558,013 + +1.5 558,013 + Pf1N1B4_465 0.53 +0.3 558,013 + Pf1N1B4_465 0.53 -0.0 558,013 + Pf1N1B4_465 0.53 -0.5 558,013 + Pf1N1B4_465 0.53 -1.6 558,013 + Pf1N1B4_465 0.53 +0.3 558,013 + Pf1N1B4_465 0.53 +1.5 558,014 - Pf1N1B4_465 0.53 +0.2 558,014 - Pf1N1B4_465 0.53 +1.3 558,014 - Pf1N1B4_465 0.53 -2.6 558,014 - -1.0 558,014 - Pf1N1B4_465 0.53 -3.0 558,014 - Pf1N1B4_465 0.53 -0.1 558,014 - Pf1N1B4_465 0.53 -1.6 558,014 - Pf1N1B4_465 0.53 -1.6 558,014 - Pf1N1B4_465 0.53 -1.0 558,019 - Pf1N1B4_465 0.54 -1.8 558,093 + Pf1N1B4_465 0.70 -0.6 558,093 + Pf1N1B4_465 0.70 -1.0 558,190 + +0.8 558,191 - -0.6 558,203 + -0.0 558,203 + +2.1 558,204 - +0.2 558,228 + -0.0 558,228 + -0.0 558,228 + +0.6 558,228 + -0.3 558,228 + +1.0 558,229 - +1.2 558,229 - +0.3 558,229 - -0.1 558,248 + -0.8 558,248 + -1.8 558,248 + +1.0 558,248 + +0.7 558,248 + -0.0 558,248 + -0.0 558,249 - +2.0 558,249 - -0.7 558,249 - -0.5 558,252 + -0.8 558,252 + -0.2 558,252 + -0.0 558,252 + -2.4 558,252 + -0.0 558,252 + +1.5 558,252 + -1.2 558,252 + -0.0 558,253 - -0.5 558,253 - -2.4 558,253 - -0.5 558,253 - -1.0 558,253 - +0.7 558,254 + -1.6 558,254 + +2.8 558,254 + -1.6 558,254 + -1.0 558,254 + -0.0 558,254 + -0.0 558,254 + -0.6 558,255 - -2.0 558,255 - -1.0 558,255 - -0.0 558,326 - +0.5 558,388 + -1.0 558,393 + -2.0 558,393 + +0.3 559,544 - Pf1N1B4_467 0.13 +0.6 559,707 - Pf1N1B4_467 0.48 -2.6 559,707 - -1.0
Or see this region's nucleotide sequence