Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4474

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4472 and Pf1N1B4_4473 overlap by 4 nucleotidesPf1N1B4_4473 and Pf1N1B4_4474 are separated by 105 nucleotidesPf1N1B4_4474 and Pf1N1B4_4475 overlap by 11 nucleotidesPf1N1B4_4475 and Pf1N1B4_4476 are separated by 135 nucleotides Pf1N1B4_4472 - Transcriptional regulator, ArsR family, at 4,933,656 to 4,934,420 _4472 Pf1N1B4_4473 - Homoserine/homoserine lactone efflux protein, at 4,934,417 to 4,935,025 _4473 Pf1N1B4_4474 - transcription regulator protein, at 4,935,131 to 4,936,057 _4474 Pf1N1B4_4475 - hypothetical protein, at 4,936,047 to 4,936,388 _4475 Pf1N1B4_4476 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 4,936,524 to 4,937,543 _4476 Position (kb) 4935 4936 4937Strain fitness (log2 ratio) -2 -1 0 1 2at 4934.541 kb on + strand, within Pf1N1B4_4473at 4934.541 kb on + strand, within Pf1N1B4_4473at 4934.542 kb on - strandat 4934.542 kb on - strandat 4934.604 kb on + strandat 4934.605 kb on - strand, within Pf1N1B4_4473at 4934.770 kb on - strand, within Pf1N1B4_4473at 4934.802 kb on + strand, within Pf1N1B4_4473at 4934.803 kb on - strand, within Pf1N1B4_4473at 4935.002 kb on + strandat 4935.002 kb on + strandat 4935.002 kb on + strandat 4935.098 kb on + strandat 4935.102 kb on + strandat 4935.111 kb on + strandat 4935.155 kb on - strandat 4935.339 kb on - strand, within Pf1N1B4_4474at 4935.339 kb on - strand, within Pf1N1B4_4474at 4935.627 kb on + strand, within Pf1N1B4_4474at 4935.628 kb on - strandat 4935.628 kb on - strand, within Pf1N1B4_4474at 4935.935 kb on + strand, within Pf1N1B4_4474at 4936.099 kb on + strand, within Pf1N1B4_4475at 4936.175 kb on + strand, within Pf1N1B4_4475at 4936.176 kb on - strand, within Pf1N1B4_4475at 4936.367 kb on - strandat 4936.432 kb on + strandat 4936.573 kb on - strandat 4936.591 kb on + strandat 4936.654 kb on + strand, within Pf1N1B4_4476at 4936.655 kb on - strand, within Pf1N1B4_4476at 4936.655 kb on - strand, within Pf1N1B4_4476at 4936.692 kb on - strand, within Pf1N1B4_4476at 4936.918 kb on + strand, within Pf1N1B4_4476at 4936.919 kb on - strand, within Pf1N1B4_4476at 4936.978 kb on + strand, within Pf1N1B4_4476at 4936.978 kb on + strand, within Pf1N1B4_4476at 4936.979 kb on - strand, within Pf1N1B4_4476at 4936.979 kb on - strand, within Pf1N1B4_4476at 4936.979 kb on - strand, within Pf1N1B4_4476

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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4,934,541 + Pf1N1B4_4473 0.20 -1.3
4,934,541 + Pf1N1B4_4473 0.20 -0.9
4,934,542 - -1.3
4,934,542 - -0.3
4,934,604 + +1.5
4,934,605 - Pf1N1B4_4473 0.31 -1.9
4,934,770 - Pf1N1B4_4473 0.58 -0.7
4,934,802 + Pf1N1B4_4473 0.63 +1.1
4,934,803 - Pf1N1B4_4473 0.63 +1.0
4,935,002 + +1.8
4,935,002 + -0.3
4,935,002 + -2.3
4,935,098 + -1.3
4,935,102 + -1.3
4,935,111 + -1.9
4,935,155 - +0.7
4,935,339 - Pf1N1B4_4474 0.22 +0.7
4,935,339 - Pf1N1B4_4474 0.22 -2.3
4,935,627 + Pf1N1B4_4474 0.54 -1.1
4,935,628 - -0.3
4,935,628 - Pf1N1B4_4474 0.54 -1.5
4,935,935 + Pf1N1B4_4474 0.87 -0.1
4,936,099 + Pf1N1B4_4475 0.15 +0.7
4,936,175 + Pf1N1B4_4475 0.37 -0.7
4,936,176 - Pf1N1B4_4475 0.38 +0.7
4,936,367 - -1.3
4,936,432 + +0.7
4,936,573 - -1.1
4,936,591 + -0.3
4,936,654 + Pf1N1B4_4476 0.13 -2.3
4,936,655 - Pf1N1B4_4476 0.13 -1.9
4,936,655 - Pf1N1B4_4476 0.13 -1.1
4,936,692 - Pf1N1B4_4476 0.16 -1.3
4,936,918 + Pf1N1B4_4476 0.39 +1.1
4,936,919 - Pf1N1B4_4476 0.39 +2.0
4,936,978 + Pf1N1B4_4476 0.45 -2.5
4,936,978 + Pf1N1B4_4476 0.45 +0.3
4,936,979 - Pf1N1B4_4476 0.45 +0.8
4,936,979 - Pf1N1B4_4476 0.45 -0.1
4,936,979 - Pf1N1B4_4476 0.45 +1.4

Or see this region's nucleotide sequence