Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4211 and Pf1N1B4_4212 are separated by 987 nucleotides Pf1N1B4_4212 and Pf1N1B4_4213 are separated by 38 nucleotides
Pf1N1B4_4211 - Transcriptional regulator, LysR family, at 4,664,560 to 4,665,474
_4211
Pf1N1B4_4212 - Gfa-like protein, at 4,666,462 to 4,666,902
_4212
Pf1N1B4_4213 - Potassium channel protein, at 4,666,941 to 4,667,816
_4213
Position (kb)
4666
4667 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4665.472 kb on + strand at 4665.555 kb on + strand at 4665.636 kb on + strand at 4665.636 kb on + strand at 4665.636 kb on + strand at 4665.636 kb on + strand at 4665.637 kb on - strand at 4665.637 kb on - strand at 4665.637 kb on - strand at 4665.893 kb on + strand at 4666.349 kb on + strand at 4666.437 kb on + strand at 4666.462 kb on - strand at 4666.492 kb on + strand at 4666.492 kb on + strand at 4666.492 kb on + strand at 4666.493 kb on - strand at 4666.503 kb on + strand at 4666.525 kb on + strand at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.730 kb on + strand, within Pf1N1B4_4212 at 4666.733 kb on + strand, within Pf1N1B4_4212 at 4666.893 kb on + strand at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.194 kb on - strand, within Pf1N1B4_4213 at 4667.197 kb on + strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.251 kb on + strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.261 kb on + strand, within Pf1N1B4_4213 at 4667.433 kb on + strand, within Pf1N1B4_4213 at 4667.434 kb on - strand, within Pf1N1B4_4213 at 4667.436 kb on - strand, within Pf1N1B4_4213 at 4667.475 kb on + strand, within Pf1N1B4_4213 at 4667.499 kb on - strand at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.757 kb on + strand at 4667.757 kb on + strand at 4667.758 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,665,472 + +0.2 4,665,555 + -0.2 4,665,636 + -0.3 4,665,636 + -1.8 4,665,636 + +1.6 4,665,636 + -0.5 4,665,637 - +0.0 4,665,637 - +0.7 4,665,637 - +0.1 4,665,893 + +0.1 4,666,349 + -1.3 4,666,437 + -0.3 4,666,462 - -1.3 4,666,492 + -0.1 4,666,492 + +1.3 4,666,492 + -0.9 4,666,493 - -0.5 4,666,503 + -2.3 4,666,525 + +0.3 4,666,730 + Pf1N1B4_4212 0.61 -1.3 4,666,730 + Pf1N1B4_4212 0.61 -1.1 4,666,730 + Pf1N1B4_4212 0.61 -1.5 4,666,730 + Pf1N1B4_4212 0.61 +0.7 4,666,733 + Pf1N1B4_4212 0.61 -0.3 4,666,893 + +1.1 4,667,049 - Pf1N1B4_4213 0.12 -2.3 4,667,049 - Pf1N1B4_4213 0.12 +0.5 4,667,094 + Pf1N1B4_4213 0.17 +1.3 4,667,094 + Pf1N1B4_4213 0.17 -0.5 4,667,188 - Pf1N1B4_4213 0.28 -1.1 4,667,188 - Pf1N1B4_4213 0.28 -3.1 4,667,194 - Pf1N1B4_4213 0.29 -0.6 4,667,197 + Pf1N1B4_4213 0.29 +1.3 4,667,198 - Pf1N1B4_4213 0.29 +0.0 4,667,198 - Pf1N1B4_4213 0.29 -0.4 4,667,249 + Pf1N1B4_4213 0.35 -3.1 4,667,249 + Pf1N1B4_4213 0.35 -0.3 4,667,251 + Pf1N1B4_4213 0.35 -1.8 4,667,252 - Pf1N1B4_4213 0.36 +0.5 4,667,252 - Pf1N1B4_4213 0.36 -1.6 4,667,252 - Pf1N1B4_4213 0.36 -0.5 4,667,252 - Pf1N1B4_4213 0.36 -1.8 4,667,252 - Pf1N1B4_4213 0.36 +0.5 4,667,261 + Pf1N1B4_4213 0.37 +1.1 4,667,433 + Pf1N1B4_4213 0.56 -1.1 4,667,434 - Pf1N1B4_4213 0.56 -1.8 4,667,436 - Pf1N1B4_4213 0.57 -0.3 4,667,475 + Pf1N1B4_4213 0.61 -1.5 4,667,499 - -0.3 4,667,653 - Pf1N1B4_4213 0.81 +0.3 4,667,653 - Pf1N1B4_4213 0.81 -1.2 4,667,696 + Pf1N1B4_4213 0.86 -0.4 4,667,696 + Pf1N1B4_4213 0.86 -0.1 4,667,757 + +0.9 4,667,757 + -0.3 4,667,758 - +0.3
Or see this region's nucleotide sequence