Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_40 and Pf1N1B4_41 are separated by 367 nucleotides Pf1N1B4_41 and Pf1N1B4_42 overlap by 4 nucleotides Pf1N1B4_42 and Pf1N1B4_43 are separated by 327 nucleotides
Pf1N1B4_40 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 46,683 to 48,254
_40
Pf1N1B4_41 - hypothetical protein, at 48,622 to 49,104
_41
Pf1N1B4_42 - hypothetical protein, at 49,101 to 50,606
_42
Pf1N1B4_43 - hypothetical protein, at 50,934 to 51,638
_43
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 48.134 kb on - strand at 48.249 kb on - strand at 48.273 kb on + strand at 48.302 kb on - strand at 48.316 kb on + strand at 48.316 kb on + strand at 48.338 kb on + strand at 48.338 kb on + strand at 48.353 kb on + strand at 48.382 kb on + strand at 48.382 kb on + strand at 48.424 kb on + strand at 48.424 kb on + strand at 48.425 kb on - strand at 48.425 kb on - strand at 48.429 kb on - strand at 48.458 kb on - strand at 48.507 kb on + strand at 48.507 kb on + strand at 48.589 kb on + strand at 48.589 kb on + strand at 48.590 kb on - strand at 48.627 kb on + strand at 48.627 kb on + strand at 48.627 kb on + strand at 48.628 kb on - strand at 48.727 kb on + strand, within Pf1N1B4_41 at 48.727 kb on + strand, within Pf1N1B4_41 at 48.727 kb on + strand, within Pf1N1B4_41 at 48.728 kb on - strand, within Pf1N1B4_41 at 48.736 kb on + strand, within Pf1N1B4_41 at 48.737 kb on - strand, within Pf1N1B4_41 at 48.737 kb on - strand, within Pf1N1B4_41 at 49.213 kb on + strand at 49.214 kb on - strand at 49.214 kb on - strand at 49.412 kb on + strand, within Pf1N1B4_42 at 49.477 kb on + strand at 49.478 kb on - strand, within Pf1N1B4_42 at 49.553 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.814 kb on - strand, within Pf1N1B4_42 at 49.829 kb on - strand, within Pf1N1B4_42 at 49.870 kb on + strand, within Pf1N1B4_42 at 49.870 kb on + strand, within Pf1N1B4_42 at 49.984 kb on + strand, within Pf1N1B4_42 at 49.984 kb on + strand, within Pf1N1B4_42 at 49.985 kb on - strand, within Pf1N1B4_42 at 49.999 kb on + strand, within Pf1N1B4_42 at 49.999 kb on + strand, within Pf1N1B4_42 at 50.000 kb on - strand, within Pf1N1B4_42 at 50.000 kb on - strand at 50.212 kb on + strand at 50.212 kb on + strand, within Pf1N1B4_42 at 50.213 kb on - strand, within Pf1N1B4_42 at 50.412 kb on - strand, within Pf1N1B4_42 at 50.595 kb on - strand at 50.710 kb on + strand at 50.841 kb on + strand at 50.904 kb on - strand at 50.959 kb on + strand at 50.987 kb on + strand at 50.987 kb on + strand at 50.987 kb on + strand at 51.128 kb on - strand at 51.142 kb on - strand, within Pf1N1B4_43 at 51.318 kb on + strand, within Pf1N1B4_43 at 51.318 kb on + strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.417 kb on + strand, within Pf1N1B4_43 at 51.418 kb on - strand at 51.418 kb on - strand, within Pf1N1B4_43 at 51.543 kb on + strand, within Pf1N1B4_43 at 51.543 kb on + strand, within Pf1N1B4_43 at 51.544 kb on - strand, within Pf1N1B4_43 at 51.579 kb on + strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.581 kb on + strand at 51.582 kb on - strand at 51.582 kb on - strand at 51.582 kb on - strand at 51.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 48,134 - -0.2 48,249 - -2.2 48,273 + +0.4 48,302 - +0.5 48,316 + +1.6 48,316 + -0.8 48,338 + +0.4 48,338 + +1.4 48,353 + +1.4 48,382 + +1.1 48,382 + -0.6 48,424 + +2.8 48,424 + -2.5 48,425 - +0.8 48,425 - -1.8 48,429 - +0.2 48,458 - -0.8 48,507 + +1.8 48,507 + -0.9 48,589 + +0.8 48,589 + -1.8 48,590 - -1.2 48,627 + -0.2 48,627 + +0.8 48,627 + -0.2 48,628 - -2.2 48,727 + Pf1N1B4_41 0.22 -1.8 48,727 + Pf1N1B4_41 0.22 -0.7 48,727 + Pf1N1B4_41 0.22 +0.5 48,728 - Pf1N1B4_41 0.22 -2.5 48,736 + Pf1N1B4_41 0.24 -0.6 48,737 - Pf1N1B4_41 0.24 -0.2 48,737 - Pf1N1B4_41 0.24 +0.0 49,213 + +0.8 49,214 - -1.2 49,214 - -0.2 49,412 + Pf1N1B4_42 0.21 -1.2 49,477 + -2.2 49,478 - Pf1N1B4_42 0.25 +0.4 49,553 + Pf1N1B4_42 0.30 +0.2 49,726 + Pf1N1B4_42 0.42 -1.2 49,726 + Pf1N1B4_42 0.42 -0.4 49,726 + Pf1N1B4_42 0.42 +1.4 49,726 + Pf1N1B4_42 0.42 +0.6 49,727 - Pf1N1B4_42 0.42 -0.8 49,727 - Pf1N1B4_42 0.42 -0.2 49,727 - Pf1N1B4_42 0.42 -1.2 49,727 - Pf1N1B4_42 0.42 +0.0 49,727 - Pf1N1B4_42 0.42 +0.0 49,727 - Pf1N1B4_42 0.42 +1.7 49,727 - Pf1N1B4_42 0.42 -1.8 49,727 - Pf1N1B4_42 0.42 +0.8 49,727 - Pf1N1B4_42 0.42 -3.5 49,727 - Pf1N1B4_42 0.42 -0.6 49,727 - Pf1N1B4_42 0.42 +0.5 49,814 - Pf1N1B4_42 0.47 +0.7 49,829 - Pf1N1B4_42 0.48 -0.5 49,870 + Pf1N1B4_42 0.51 +0.8 49,870 + Pf1N1B4_42 0.51 -2.2 49,984 + Pf1N1B4_42 0.59 -1.2 49,984 + Pf1N1B4_42 0.59 -0.2 49,985 - Pf1N1B4_42 0.59 -0.5 49,999 + Pf1N1B4_42 0.60 +0.2 49,999 + Pf1N1B4_42 0.60 -0.2 50,000 - Pf1N1B4_42 0.60 -0.2 50,000 - -1.8 50,212 + +3.1 50,212 + Pf1N1B4_42 0.74 -0.6 50,213 - Pf1N1B4_42 0.74 +0.8 50,412 - Pf1N1B4_42 0.87 +0.7 50,595 - -0.4 50,710 + +2.6 50,841 + -2.2 50,904 - +1.1 50,959 + +0.8 50,987 + -0.2 50,987 + -3.2 50,987 + -1.5 51,128 - +0.8 51,142 - Pf1N1B4_43 0.30 +0.9 51,318 + Pf1N1B4_43 0.54 +0.8 51,318 + Pf1N1B4_43 0.54 -0.4 51,319 - Pf1N1B4_43 0.55 +1.8 51,319 - Pf1N1B4_43 0.55 +0.8 51,319 - Pf1N1B4_43 0.55 +0.1 51,319 - Pf1N1B4_43 0.55 +0.6 51,319 - Pf1N1B4_43 0.55 +1.2 51,319 - Pf1N1B4_43 0.55 -0.6 51,319 - Pf1N1B4_43 0.55 +1.0 51,319 - Pf1N1B4_43 0.55 +1.4 51,319 - Pf1N1B4_43 0.55 +0.8 51,319 - Pf1N1B4_43 0.55 +0.9 51,417 + Pf1N1B4_43 0.69 -0.2 51,418 - -0.2 51,418 - Pf1N1B4_43 0.69 +1.6 51,543 + Pf1N1B4_43 0.86 -2.8 51,543 + Pf1N1B4_43 0.86 -0.0 51,544 - Pf1N1B4_43 0.87 +0.1 51,579 + -1.2 51,580 - -0.1 51,580 - +2.3 51,580 - +0.1 51,580 - +1.1 51,580 - -0.2 51,580 - +2.1 51,581 + -0.5 51,582 - +0.6 51,582 - +0.8 51,582 - +1.2 51,582 - +2.4
Or see this region's nucleotide sequence