Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotides Pf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotides Pf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides Pf1N1B4_4051 and Pf1N1B4_4052 are separated by 493 nucleotides
Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589
_4048
Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095
_4049
Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422
_4050
Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533
_4051
Pf1N1B4_4052 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,505,027 to 4,506,283
_4052
Position (kb)
4502
4503
4504
4505 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4501.709 kb on - strand at 4501.709 kb on - strand, within Pf1N1B4_4049 at 4501.806 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.855 kb on - strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4502.239 kb on + strand at 4502.239 kb on + strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.248 kb on - strand at 4502.248 kb on - strand at 4502.302 kb on + strand at 4502.310 kb on + strand at 4502.551 kb on - strand at 4502.663 kb on - strand at 4502.673 kb on + strand, within Pf1N1B4_4051 at 4502.674 kb on - strand, within Pf1N1B4_4051 at 4502.674 kb on - strand, within Pf1N1B4_4051 at 4502.678 kb on - strand, within Pf1N1B4_4051 at 4503.004 kb on - strand, within Pf1N1B4_4051 at 4503.020 kb on + strand, within Pf1N1B4_4051 at 4503.020 kb on + strand, within Pf1N1B4_4051 at 4503.389 kb on + strand, within Pf1N1B4_4051 at 4503.566 kb on + strand, within Pf1N1B4_4051 at 4503.625 kb on - strand, within Pf1N1B4_4051 at 4503.710 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.073 kb on + strand, within Pf1N1B4_4051 at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.472 kb on + strand at 4504.506 kb on + strand at 4504.506 kb on + strand at 4504.508 kb on + strand at 4504.508 kb on + strand at 4504.560 kb on + strand at 4504.692 kb on - strand at 4504.727 kb on + strand at 4504.769 kb on + strand at 4504.770 kb on - strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.771 kb on + strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.772 kb on - strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.775 kb on + strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.776 kb on - strand at 4504.780 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.805 kb on - strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.830 kb on + strand at 4504.856 kb on + strand at 4504.856 kb on + strand at 4504.910 kb on + strand at 4504.910 kb on + strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.911 kb on - strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.952 kb on + strand at 4504.953 kb on - strand at 4504.971 kb on + strand at 4504.972 kb on - strand at 4504.972 kb on - strand at 4504.981 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.022 kb on - strand at 4505.140 kb on + strand at 4505.141 kb on - strand at 4505.153 kb on - strand, within Pf1N1B4_4052 at 4505.237 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.315 kb on + strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.316 kb on - strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand, within Pf1N1B4_4052 at 4505.319 kb on + strand at 4505.320 kb on - strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand, within Pf1N1B4_4052 at 4505.405 kb on + strand at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.406 kb on - strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.510 kb on + strand, within Pf1N1B4_4052 at 4505.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 4,501,709 - -1.2 4,501,709 - Pf1N1B4_4049 0.13 -0.3 4,501,806 + Pf1N1B4_4049 0.35 +0.4 4,501,854 + Pf1N1B4_4049 0.46 +0.2 4,501,854 + Pf1N1B4_4049 0.46 +0.5 4,501,855 - Pf1N1B4_4049 0.46 -1.0 4,501,881 + Pf1N1B4_4049 0.52 +2.6 4,501,881 + Pf1N1B4_4049 0.52 -2.2 4,501,881 + Pf1N1B4_4049 0.52 +1.1 4,501,887 + -0.2 4,501,887 + Pf1N1B4_4049 0.53 -1.2 4,501,887 + Pf1N1B4_4049 0.53 -0.2 4,501,887 + Pf1N1B4_4049 0.53 -1.2 4,501,887 + Pf1N1B4_4049 0.53 +0.2 4,502,239 + -0.6 4,502,239 + +0.8 4,502,240 - -0.5 4,502,240 - -0.2 4,502,240 - +1.3 4,502,240 - -0.6 4,502,240 - +0.3 4,502,240 - +0.5 4,502,240 - -0.6 4,502,240 - +1.8 4,502,240 - +1.5 4,502,240 - -0.8 4,502,240 - -1.2 4,502,243 + +0.5 4,502,243 + +0.5 4,502,243 + -1.0 4,502,243 + -1.8 4,502,243 + -2.2 4,502,243 + -2.2 4,502,243 + +0.4 4,502,243 + -1.8 4,502,243 + +1.6 4,502,243 + +1.8 4,502,243 + +0.8 4,502,243 + +0.2 4,502,243 + -1.2 4,502,243 + +0.1 4,502,244 - -2.5 4,502,244 - +0.1 4,502,244 - -2.2 4,502,244 - +1.5 4,502,244 - -0.8 4,502,247 + -1.2 4,502,247 + +0.8 4,502,247 + +0.8 4,502,247 + +0.4 4,502,248 - -1.2 4,502,248 - +0.8 4,502,302 + -0.8 4,502,310 + +1.2 4,502,551 - -2.2 4,502,663 - -0.2 4,502,673 + Pf1N1B4_4051 0.10 -0.2 4,502,674 - Pf1N1B4_4051 0.10 -1.8 4,502,674 - Pf1N1B4_4051 0.10 -2.2 4,502,678 - Pf1N1B4_4051 0.10 -0.5 4,503,004 - Pf1N1B4_4051 0.26 +1.1 4,503,020 + Pf1N1B4_4051 0.27 -0.4 4,503,020 + Pf1N1B4_4051 0.27 -1.4 4,503,389 + Pf1N1B4_4051 0.45 -1.8 4,503,566 + Pf1N1B4_4051 0.53 -1.6 4,503,625 - Pf1N1B4_4051 0.56 -3.5 4,503,710 + Pf1N1B4_4051 0.60 -1.2 4,504,073 + Pf1N1B4_4051 0.78 -2.4 4,504,073 + Pf1N1B4_4051 0.78 -2.2 4,504,472 + -1.4 4,504,472 + -2.0 4,504,472 + +0.8 4,504,506 + -1.2 4,504,506 + -1.2 4,504,508 + -2.0 4,504,508 + -0.4 4,504,560 + -0.2 4,504,692 - -0.8 4,504,727 + -0.2 4,504,769 + -1.2 4,504,770 - -0.9 4,504,771 + -1.5 4,504,771 + -2.2 4,504,771 + -1.4 4,504,771 + -2.5 4,504,771 + -1.4 4,504,771 + -1.2 4,504,771 + -2.5 4,504,771 + -0.2 4,504,771 + -2.5 4,504,771 + +0.4 4,504,771 + -1.0 4,504,771 + -1.2 4,504,771 + -0.2 4,504,771 + -0.3 4,504,771 + -0.7 4,504,771 + +1.8 4,504,771 + -1.7 4,504,772 - +0.1 4,504,772 - +0.4 4,504,772 - -0.2 4,504,772 - +0.6 4,504,772 - +1.2 4,504,772 - -0.2 4,504,775 + -2.5 4,504,775 + -1.8 4,504,775 + -1.4 4,504,775 + -0.1 4,504,775 + -1.4 4,504,775 + -1.2 4,504,775 + +1.4 4,504,775 + +2.6 4,504,776 - +2.1 4,504,776 - -1.8 4,504,776 - -1.8 4,504,776 - -1.0 4,504,776 - -0.4 4,504,776 - +0.7 4,504,776 - +1.8 4,504,780 - -0.5 4,504,805 - -0.2 4,504,805 - -0.2 4,504,805 - +0.1 4,504,830 + -1.4 4,504,830 + -1.6 4,504,830 + -1.4 4,504,856 + +0.4 4,504,856 + +0.8 4,504,910 + +1.4 4,504,910 + +0.4 4,504,911 - -0.2 4,504,911 - +2.0 4,504,911 - +1.8 4,504,911 - -0.5 4,504,911 - -0.7 4,504,911 - +1.4 4,504,952 + +0.6 4,504,952 + +0.0 4,504,952 + +0.1 4,504,952 + -0.8 4,504,952 + -0.6 4,504,953 - +1.4 4,504,971 + +1.7 4,504,972 - -0.2 4,504,972 - +0.1 4,504,981 - +0.1 4,505,022 - -0.2 4,505,022 - -0.8 4,505,022 - +3.0 4,505,140 + +0.2 4,505,141 - -1.3 4,505,153 - Pf1N1B4_4052 0.10 -1.2 4,505,237 + Pf1N1B4_4052 0.17 -1.4 4,505,315 + Pf1N1B4_4052 0.23 -0.5 4,505,315 + Pf1N1B4_4052 0.23 -0.1 4,505,315 + Pf1N1B4_4052 0.23 +0.6 4,505,315 + Pf1N1B4_4052 0.23 +0.8 4,505,316 - Pf1N1B4_4052 0.23 -0.8 4,505,316 - Pf1N1B4_4052 0.23 +0.3 4,505,319 + Pf1N1B4_4052 0.23 +0.1 4,505,319 + Pf1N1B4_4052 0.23 +0.5 4,505,319 + -0.2 4,505,320 - Pf1N1B4_4052 0.23 +3.8 4,505,405 + Pf1N1B4_4052 0.30 +0.8 4,505,405 + Pf1N1B4_4052 0.30 -1.8 4,505,405 + Pf1N1B4_4052 0.30 +1.0 4,505,405 + Pf1N1B4_4052 0.30 +2.0 4,505,405 + Pf1N1B4_4052 0.30 -1.7 4,505,405 + -0.2 4,505,406 - Pf1N1B4_4052 0.30 +0.5 4,505,406 - Pf1N1B4_4052 0.30 -0.8 4,505,510 + Pf1N1B4_4052 0.38 +0.2 4,505,510 + Pf1N1B4_4052 0.38 +0.3 4,505,510 + Pf1N1B4_4052 0.38 -0.2 4,505,511 - +1.4
Or see this region's nucleotide sequence