Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4030

Experiment: Cisplatin 0.006 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4029 and Pf1N1B4_4030 are separated by 39 nucleotidesPf1N1B4_4030 and Pf1N1B4_4031 overlap by 4 nucleotidesPf1N1B4_4031 and Pf1N1B4_4032 are separated by 2 nucleotidesPf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotides Pf1N1B4_4029 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 4,479,723 to 4,481,255 _4029 Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148 _4030 Pf1N1B4_4031 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 4,483,145 to 4,483,453 _4031 Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959 _4032 Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514 _4033 Position (kb) 4481 4482 4483 4484Strain fitness (log2 ratio) -3 -2 -1 0 1at 4480.352 kb on - strand, within Pf1N1B4_4029at 4480.444 kb on + strand, within Pf1N1B4_4029at 4480.444 kb on + strand, within Pf1N1B4_4029at 4480.588 kb on + strand, within Pf1N1B4_4029at 4480.801 kb on + strand, within Pf1N1B4_4029at 4480.802 kb on - strand, within Pf1N1B4_4029at 4481.325 kb on + strandat 4481.861 kb on - strand, within Pf1N1B4_4030at 4481.861 kb on - strand, within Pf1N1B4_4030at 4481.954 kb on - strand, within Pf1N1B4_4030at 4482.168 kb on + strand, within Pf1N1B4_4030at 4482.215 kb on - strand, within Pf1N1B4_4030at 4482.826 kb on + strand, within Pf1N1B4_4030at 4482.923 kb on + strand, within Pf1N1B4_4030at 4483.005 kb on + strandat 4483.006 kb on - strandat 4483.198 kb on - strand, within Pf1N1B4_4031at 4483.634 kb on + strand, within Pf1N1B4_4032at 4483.635 kb on - strand, within Pf1N1B4_4032at 4484.060 kb on + strand, within Pf1N1B4_4033at 4484.061 kb on - strandat 4484.069 kb on + strand, within Pf1N1B4_4033at 4484.070 kb on - strand, within Pf1N1B4_4033at 4484.070 kb on - strand, within Pf1N1B4_4033

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml
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4,480,352 - Pf1N1B4_4029 0.41 +1.4
4,480,444 + Pf1N1B4_4029 0.47 +1.5
4,480,444 + Pf1N1B4_4029 0.47 -3.4
4,480,588 + Pf1N1B4_4029 0.56 -0.2
4,480,801 + Pf1N1B4_4029 0.70 -0.2
4,480,802 - Pf1N1B4_4029 0.70 -2.2
4,481,325 + +0.4
4,481,861 - Pf1N1B4_4030 0.31 +0.4
4,481,861 - Pf1N1B4_4030 0.31 -1.4
4,481,954 - Pf1N1B4_4030 0.36 +0.2
4,482,168 + Pf1N1B4_4030 0.47 -2.2
4,482,215 - Pf1N1B4_4030 0.50 -1.0
4,482,826 + Pf1N1B4_4030 0.83 -0.2
4,482,923 + Pf1N1B4_4030 0.88 -2.7
4,483,005 + +0.2
4,483,006 - -1.2
4,483,198 - Pf1N1B4_4031 0.17 -1.8
4,483,634 + Pf1N1B4_4032 0.35 -2.9
4,483,635 - Pf1N1B4_4032 0.36 -0.6
4,484,060 + Pf1N1B4_4033 0.17 +0.2
4,484,061 - -1.8
4,484,069 + Pf1N1B4_4033 0.18 -0.2
4,484,070 - Pf1N1B4_4033 0.18 +0.4
4,484,070 - Pf1N1B4_4033 0.18 -1.6

Or see this region's nucleotide sequence