Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3545 and Pf1N1B4_3546 are separated by 175 nucleotides Pf1N1B4_3546 and Pf1N1B4_3547 are separated by 363 nucleotides Pf1N1B4_3547 and Pf1N1B4_3548 are separated by 7 nucleotides Pf1N1B4_3548 and Pf1N1B4_3549 are separated by 110 nucleotides Pf1N1B4_3549 and Pf1N1B4_3550 are separated by 1 nucleotides
Pf1N1B4_3545 - Putative two-component system sensor kinase, at 3,951,256 to 3,952,728
_3545
Pf1N1B4_3546 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 3,952,904 to 3,953,353
_3546
Pf1N1B4_3547 - DNA-binding response regulator, at 3,953,717 to 3,954,394
_3547
Pf1N1B4_3548 - Translation initiation factor 2 (IF-2; GTPase), at 3,954,402 to 3,954,815
_3548
Pf1N1B4_3549 - YciL protein, at 3,954,926 to 3,955,225
_3549
Pf1N1B4_3550 - Intracellular septation protein IspA, at 3,955,227 to 3,955,823
_3550
Position (kb)
3953
3954
3955 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3952.808 kb on - strand at 3952.848 kb on + strand at 3952.848 kb on + strand at 3952.875 kb on + strand at 3952.875 kb on + strand at 3952.876 kb on - strand at 3952.876 kb on - strand at 3952.906 kb on - strand at 3953.112 kb on + strand, within Pf1N1B4_3546 at 3953.113 kb on - strand, within Pf1N1B4_3546 at 3953.113 kb on - strand at 3953.358 kb on + strand at 3953.358 kb on + strand at 3953.359 kb on - strand at 3953.359 kb on - strand at 3953.359 kb on - strand at 3953.359 kb on - strand at 3953.437 kb on + strand at 3953.437 kb on + strand at 3953.438 kb on - strand at 3953.438 kb on - strand at 3953.438 kb on - strand at 3953.520 kb on + strand at 3953.521 kb on - strand at 3953.521 kb on - strand at 3953.614 kb on - strand at 3953.660 kb on + strand at 3953.701 kb on - strand at 3953.727 kb on + strand at 3953.727 kb on + strand at 3953.728 kb on - strand at 3953.730 kb on + strand at 3953.730 kb on + strand at 3953.730 kb on + strand at 3953.730 kb on + strand at 3953.731 kb on - strand at 3953.731 kb on - strand at 3953.731 kb on - strand at 3953.731 kb on - strand at 3953.731 kb on - strand at 3953.733 kb on + strand at 3953.832 kb on + strand, within Pf1N1B4_3547 at 3953.833 kb on - strand, within Pf1N1B4_3547 at 3954.100 kb on - strand, within Pf1N1B4_3547 at 3954.340 kb on + strand at 3954.340 kb on + strand at 3954.341 kb on - strand at 3954.377 kb on + strand at 3954.377 kb on + strand at 3954.377 kb on + strand at 3954.378 kb on - strand at 3954.378 kb on - strand at 3954.378 kb on - strand at 3954.380 kb on + strand at 3954.381 kb on - strand at 3954.474 kb on + strand, within Pf1N1B4_3548 at 3954.622 kb on + strand, within Pf1N1B4_3548 at 3954.624 kb on - strand, within Pf1N1B4_3548 at 3954.834 kb on + strand at 3954.873 kb on - strand at 3954.987 kb on + strand, within Pf1N1B4_3549 at 3954.987 kb on + strand, within Pf1N1B4_3549 at 3954.988 kb on - strand, within Pf1N1B4_3549 at 3954.988 kb on - strand at 3954.988 kb on - strand, within Pf1N1B4_3549 at 3955.112 kb on + strand, within Pf1N1B4_3549 at 3955.113 kb on - strand, within Pf1N1B4_3549 at 3955.133 kb on - strand at 3955.133 kb on - strand, within Pf1N1B4_3549 at 3955.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,952,808 - +0.6 3,952,848 + +0.9 3,952,848 + -0.1 3,952,875 + +1.1 3,952,875 + -1.1 3,952,876 - +0.1 3,952,876 - -0.1 3,952,906 - -0.1 3,953,112 + Pf1N1B4_3546 0.46 -0.7 3,953,113 - Pf1N1B4_3546 0.46 +0.1 3,953,113 - +0.9 3,953,358 + -1.3 3,953,358 + -0.3 3,953,359 - -0.1 3,953,359 - -1.9 3,953,359 - -0.0 3,953,359 - -0.1 3,953,437 + -1.1 3,953,437 + +1.4 3,953,438 - +0.9 3,953,438 - +0.1 3,953,438 - -2.7 3,953,520 + -0.4 3,953,521 - -1.7 3,953,521 - +2.9 3,953,614 - +0.3 3,953,660 + -0.7 3,953,701 - -0.1 3,953,727 + -3.3 3,953,727 + -1.4 3,953,728 - +2.5 3,953,730 + +0.2 3,953,730 + +1.3 3,953,730 + +0.8 3,953,730 + +0.5 3,953,731 - +0.2 3,953,731 - +0.7 3,953,731 - +1.7 3,953,731 - -0.7 3,953,731 - -0.7 3,953,733 + -1.7 3,953,832 + Pf1N1B4_3547 0.17 -0.3 3,953,833 - Pf1N1B4_3547 0.17 -2.0 3,954,100 - Pf1N1B4_3547 0.56 -1.5 3,954,340 + -0.8 3,954,340 + +1.7 3,954,341 - +0.2 3,954,377 + -2.4 3,954,377 + -2.1 3,954,377 + -0.1 3,954,378 - +1.1 3,954,378 - +0.5 3,954,378 - -0.7 3,954,380 + +0.7 3,954,381 - +0.6 3,954,474 + Pf1N1B4_3548 0.17 -0.1 3,954,622 + Pf1N1B4_3548 0.53 +2.2 3,954,624 - Pf1N1B4_3548 0.54 +1.4 3,954,834 + -1.7 3,954,873 - +0.7 3,954,987 + Pf1N1B4_3549 0.20 +0.9 3,954,987 + Pf1N1B4_3549 0.20 +0.6 3,954,988 - Pf1N1B4_3549 0.21 +1.2 3,954,988 - +1.9 3,954,988 - Pf1N1B4_3549 0.21 -0.1 3,955,112 + Pf1N1B4_3549 0.62 +2.2 3,955,113 - Pf1N1B4_3549 0.62 +2.5 3,955,133 - -0.1 3,955,133 - Pf1N1B4_3549 0.69 +0.9 3,955,219 - +0.2
Or see this region's nucleotide sequence