Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3523 and Pf1N1B4_3524 are separated by 110 nucleotides Pf1N1B4_3524 and Pf1N1B4_3525 overlap by 4 nucleotides Pf1N1B4_3525 and Pf1N1B4_3526 are separated by 50 nucleotides Pf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides
Pf1N1B4_3523 - hypothetical protein, at 3,929,988 to 3,930,125
_3523
Pf1N1B4_3524 - FIG00955363: hypothetical protein, at 3,930,236 to 3,931,042
_3524
Pf1N1B4_3525 - hydrolase, alpha/beta fold family, at 3,931,039 to 3,931,893
_3525
Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603
_3526
Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418
_3527
Position (kb)
3931
3932 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3930.133 kb on + strand at 3930.181 kb on + strand at 3930.235 kb on + strand at 3930.235 kb on + strand at 3930.235 kb on + strand at 3930.235 kb on + strand at 3930.236 kb on - strand at 3930.236 kb on - strand at 3930.236 kb on - strand at 3930.236 kb on - strand at 3930.249 kb on + strand at 3930.250 kb on - strand at 3930.250 kb on - strand at 3930.250 kb on - strand at 3930.288 kb on - strand at 3930.288 kb on - strand at 3930.321 kb on - strand, within Pf1N1B4_3524 at 3930.674 kb on - strand at 3930.674 kb on - strand, within Pf1N1B4_3524 at 3930.716 kb on + strand, within Pf1N1B4_3524 at 3930.716 kb on + strand, within Pf1N1B4_3524 at 3930.791 kb on + strand, within Pf1N1B4_3524 at 3930.792 kb on - strand, within Pf1N1B4_3524 at 3930.792 kb on - strand, within Pf1N1B4_3524 at 3930.792 kb on - strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3930.949 kb on + strand, within Pf1N1B4_3524 at 3931.299 kb on - strand, within Pf1N1B4_3525 at 3931.431 kb on + strand, within Pf1N1B4_3525 at 3931.516 kb on + strand at 3931.516 kb on + strand, within Pf1N1B4_3525 at 3931.516 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.629 kb on + strand, within Pf1N1B4_3525 at 3931.630 kb on - strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.948 kb on + strand at 3931.949 kb on - strand at 3931.949 kb on - strand at 3931.956 kb on + strand at 3931.956 kb on + strand at 3932.164 kb on - strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.352 kb on + strand, within Pf1N1B4_3526 at 3932.403 kb on + strand, within Pf1N1B4_3526 at 3932.427 kb on + strand, within Pf1N1B4_3526 at 3932.608 kb on - strand at 3932.672 kb on - strand at 3932.849 kb on + strand at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 3,930,133 + -0.1 3,930,181 + -2.1 3,930,235 + -0.1 3,930,235 + -1.7 3,930,235 + -0.1 3,930,235 + +0.4 3,930,236 - +0.2 3,930,236 - +0.8 3,930,236 - +1.7 3,930,236 - -0.3 3,930,249 + +0.9 3,930,250 - -0.1 3,930,250 - -2.1 3,930,250 - -2.4 3,930,288 - -0.1 3,930,288 - -0.8 3,930,321 - Pf1N1B4_3524 0.11 -1.3 3,930,674 - -0.1 3,930,674 - Pf1N1B4_3524 0.54 -0.1 3,930,716 + Pf1N1B4_3524 0.59 +0.7 3,930,716 + Pf1N1B4_3524 0.59 -2.4 3,930,791 + Pf1N1B4_3524 0.69 +0.9 3,930,792 - Pf1N1B4_3524 0.69 -0.2 3,930,792 - Pf1N1B4_3524 0.69 -1.7 3,930,792 - Pf1N1B4_3524 0.69 -0.5 3,930,949 + Pf1N1B4_3524 0.88 +0.3 3,930,949 + Pf1N1B4_3524 0.88 -0.4 3,930,949 + Pf1N1B4_3524 0.88 -1.4 3,931,299 - Pf1N1B4_3525 0.30 +0.9 3,931,431 + Pf1N1B4_3525 0.46 -0.1 3,931,516 + +0.9 3,931,516 + Pf1N1B4_3525 0.56 -0.8 3,931,516 + Pf1N1B4_3525 0.56 +2.7 3,931,629 + Pf1N1B4_3525 0.69 +1.9 3,931,629 + Pf1N1B4_3525 0.69 +0.7 3,931,630 - -0.1 3,931,948 + -0.6 3,931,948 + +0.5 3,931,948 + -0.1 3,931,949 - -0.1 3,931,949 - -0.1 3,931,956 + +0.1 3,931,956 + -0.1 3,932,164 - Pf1N1B4_3526 0.33 +1.1 3,932,352 + Pf1N1B4_3526 0.62 -1.1 3,932,352 + Pf1N1B4_3526 0.62 +0.3 3,932,352 + Pf1N1B4_3526 0.62 +0.3 3,932,352 + Pf1N1B4_3526 0.62 +0.8 3,932,352 + Pf1N1B4_3526 0.62 -1.7 3,932,352 + Pf1N1B4_3526 0.62 +0.4 3,932,352 + Pf1N1B4_3526 0.62 -1.4 3,932,403 + Pf1N1B4_3526 0.70 -1.7 3,932,427 + Pf1N1B4_3526 0.73 +1.1 3,932,608 - -0.8 3,932,672 - -2.1 3,932,849 + +1.9 3,932,850 - Pf1N1B4_3527 0.29 -1.7 3,932,850 - Pf1N1B4_3527 0.29 -0.1 3,932,850 - Pf1N1B4_3527 0.29 -1.7
Or see this region's nucleotide sequence